A: Our project management team will send progress emails every week. If you do not receive the email or are in a hurry, you can email service@synbio-tech.com or contact the corresponding sales representative for inquiry.

A: Throughout the entire gene synthesis project, we adopt strict and effective quality control measures to ensure the accuracy of the synthesized gene. Firstly, before the project starts, the project manager will analyze your sequence, write the order according to your requirements, and then ask you to verify whether the sequence information and requirements in the order are correct. For the shipped plasmid, we will ensure 100% correctness through Sanger sequencing, and at the same time perform restriction enzyme digestion verification on the plasmid to ensure that the bands fully meet the expectations. The QC department will carefully review the shipped samples, and the samples will be shipped only after passing the review. Finally, the shipping documents of the order and the courier tracking number of the shipping list will be sent to your email address in the form of an email.

A: Plasmids can be stored for one year, while bacterial cultures will not be stored. If the customer requires storage for more than one year, it can be specified in the contract, and an additional fee will be charged to extend the storage time. The fee is $300. After one year, each time the customer uses them, a fee of $100 will be charged, with a maximum of one charge per year, and no fee will be charged if they are not used.

A: Yes, the pSmart-I plasmid.

A: This service belongs to the Trimer primer synthesis service. Specifically, the following need to be confirmed: 1. Is it to synthesize a library or just to do primer synthesis? 2. Specific sequence information and mutation site information, including the number of sites to be mutated, and whether the mutation is saturation mutation or has specified requirements on the proportion of mutated amino acids? 3. The full-length sequence and vector information need to be confirmed.

A: For gene synthesis, customers need to provide plasmids.

A: Yes, it is gene synthesis. We only need customers to provide the heavy chain and light chain sequences of the antibody.

A: Our current antibody expression vectors are murine and human-derived. We can synthesize the full-length antibody sequences of goat, chicken or sheep and clone them into pTT5 for expression.

A: About 5μg is needed.

A: There are lentiviral vectors: pCDH-CMV-MCS-EF1-copGFP and pCDH-CMV-MCS-EF1-Puro. You can ask about the customer's research purpose. If the customer is working on proteins, these two vectors are not recommended.

A: For transfected cells, flag is generally used to detect the expression. If there is a tag, WB can be performed to identify whether it is expressed. Fluorescent vectors or QPCR can be used to detect whether the cells are transfected. If the plasmid is not labeled with fluorescence, QPCR can be used for detection.

1. Primers: Try replacing the primers, and set up positive controls (other plasmids with the same vector) and negative controls (empty vector) to check if the problem is with the primers.

2. PCR amplification system: Usually, there is little problem, but you can check information such as annealing temperature.

3. Whether the antibiotic resistance was added incorrectly, and whether the antibiotic concentration is normal.