In Vitro Transcription | Synbio Technologies

Home > RNA Synthesis > In Vitro Transcription

General Introduction of In Vitro Transcription

In vitro transcription utilizes linear DNA templates with RNA polymerases (e.g., T7, T3, SP6) to synthesize RNA. Following this synthesis, we employ a comprehensive Lipid Nanoparticle (LNP) platform, where molecular self-assembly creates stable nanoparticle systems that significantly enhance the stability and targeting efficacy of the RNA for delivery.

Synbio Technologies offers efficient and cost-effective in vitro transcription RNA synthesis through our Syno synthesis platform. Our end-to-end service covers gene synthesis, plasmid preparation, RNA synthesis, and LNP encapsulation, offering scalable production support for innovative drug development.

Highlights

One-Stop Service from Sequence to LNP products
Fully Customized Synthesis
Guaranteed Service: No RNA, No Pay
Free Codon Optimization
> 95% Purity & Strict QC
Ready-to-Use mRNA-LNPs：150+ pre-validated mRNA sequence

Service Details

Steps	Service Details	Turnaround Time
AI Design & Optimization	• AI Sequence Design • Sequence Analysis • Codon Optimization	3-5 Days
Gene Synthesis	• Gene Synthesis • Vector Construction	Start from 1 Week，According to the sequence
mRNASynthesis	linear mRNA / circular RNA / self-amplified RNA	Start from 1 Week
LNP Encapsulation	Specification : 100μg-100mg Encapsulation length : 100nt ~ 20kb mRNA	Start from 1 Week
Detection Services	RNA quality inspection LNP antibody coupling detection	Start from 1 Week

Size/Scale	PolyA Tails	Cap	Modifications	Purifications	Turnaround Time	Price
40-9000 nt 2 µg-g	• Customized tail • 100-150A tail (enzymatic method) • 120A tail • No tail	• One stop capping (Clean cap AG, ARCA cap, m7GpppG Cap) • Enzymatic capping (Cap 0, 1) • No cap	• 5-mUTP • 5-moUTP • ψ-UTP • N1-Me-ψ-UTP • Cy3, Cy5, AF488 • Unmodified	• Silica column purification • LiCl purification • Phenol/chloroform extraction • HPLC	IVT: 5-10 BD Gene Synthesis: 10-15 BD	2 µg start from $200 1 mg start from $1,000

* The price only refers to the transcription, gene synthesis price and modification will be charged separately.

Quality Control

QC Items*	Method	Specifications	Default QC	QC Price
Appearance	Visual inspection	Clear, colorless, free from visible particulates	√	Free
A 260/280 Ratio	UV Absorbance	1.70 - 2.30	√	Free
Quantity	UV Absorbance	Quantity is ± 5%	√	Free
RNA size & purity*	Denaturing agarose gel electrophoresis	Detect expected size band	√	Free
Endotoxin	TAL assay	< 10 EU/mg	X	$50 per reaction
DNA template residue test	PCR & Gel electrophoresis	PCR product from the RNA products is <5% compared to positive control	X	$125 per reaction
Bioburden	Streak inoculation	No growth after 48 hours	X	$50 per reaction
Mycoplasma	Mycoplasma PCR Detection	No mycoplasma is detected in the RNA products	X	$125 per reaction
RNA Sequence confirm	RT-PCR & Sanger sequencing	Sequencing results are consistent with the confirmed RNA sequence	X	Starting at $250
Functional test	Transfection & Western Blot	Detect expected size protein	X	Quote

* For more QC options, please contact us at quote@synbio-tech.com.

Order Now Quote

Workflow

Order Now

Templates For In Vitro Transcription

View More

dsDNA templates often increase transcription yield of the RNA. Synbio Technologies provides multiple types of DNA templates for in vitro transcription, including the linearized DNA plasmids, PCR products, synthetic oligos, and cDNA.

Deliverables

Liquid RNA

COA

Lyophilized template plasmid

Case Study

RNA (1010 nt) on denaturing agarose gel. The RNA was synthesized by in vitro transcription and purified via LiCl purification method.

FAQs

How do I ensure that the synthesized mRNA has high purity?

High purity mRNA can be obtained by optimizing in vitro transcription conditions and using efficient purification methods (e.g. column chromatography, gel electrophoresis, etc.).

Why is 5‘ capping and 3’ tailing required for mRNA synthesis?

5‘ capping and 3’ tailing help to improve the stability and translation efficiency of mRNA and prevent it from being degraded inside the cell.

How should I store my RNA products?

mRNA can remain stable for at least one year if stored at -80°C and not subjected to repeated freeze-thaw cycles, though stability varies with mRNA length. For long-term storage, keep mRNA at -80°C; for short-term, -20°C is recommended.

More FAQs>>

Resources

Brochure & Flyers