1. Membrane Protein Expression

• Detergent-Supplemented Cell-Free Platform（No Expression No Charge）

Leveraging its proprietary CFPS platform, Synbio Technologies achieves soluble expression of full-length transmembrane proteins by supplementing detergents directly into the cell-free system. These detergents create a hydrophobic environment that stabilizes transmembrane domains and promotes proper folding into native conformations.

STEAP1 membrane protein schematic diagram in the form of detergent (left) and SDS-PAGE (right)  

• Nanodisc-Supplemented Cell-Free Platform

By supplementing membrane scaffold proteins and phospholipids, Synbio Technologies’ cell-free system drives nanodisc self-assembly that mimics native membranes. Newly synthesized proteins integrate directly, with transmembrane domains stably embedded in the bilayer, yielding full-length proteins with improved stability, activity, and near-native conformations.

STEAP1 membrane protein schematic diagram in the form of nanodisc(left) and SDS-PAGE diagram (right)

• Cell-free expression + BLI detection

BLI (Bio-Layer Interferometry) is an optical analytical technique for real-time monitoring of biomolecular interactions. It enables label-free, real-time affinity detection between two biomolecules, offering advantages such as simple operation, real-time monitoring, high throughput, and detergent tolerance, making it particularly suitable for assessing the antigenic activity of membrane proteins.

The results show that STEAP1 membrane protein in the form of detergent binds stably to the probe and exhibits strong specific antibody binding, while the blank control confirms no nonspecific interactions and a clean background.

The results show that STEAP1 membrane protein in nanodiscs binds well to the probe with clear specific antibody signals, while the blank control with 0.05% Tween 20 confirms no nonspecific interactions.

Affinity results of detergent-solubilized STEAP1 membrane protein with antibodies at different concentration gradients

Affinity results of nanodisc-form STEAP1 membrane protein with antibodies at different concentration gradients

No nonspecific binding observed between membrane scaffold protein and STEAP1 antibody

The results show that detergent-solubilized STEAP1 membrane protein binds its antibody with ~0.619 nM affinity, whereas nanodisc-form STEAP1 achieves a remarkable 0.0782 nM, with no nonspecific binding to the scaffold protein.

2. Hard-to-express Proteins

Synbio Technologies enables rapid, high-efficiency synthesis of even the most challenging proteins, including membrane proteins—achieving success rates above 96%, fastest delivery in just 3 days, and a no-expression-no-charge guarantee.

Cell-free expression of difficulty protein

Cell-free expression of IL-2 vs. other brands of IL-2 activity

Cell-free expression of antibody electrophoresis and ELISA binding activity

3. Non-natural amino acid insertion

Synbio Technologies offers site-specific non-natural amino acid (nnAA) incorporation using its proprietary cell-free protein expression platform, enabling rapid, high-efficiency synthesis of proteins with precisely inserted nnAAs, demonstrated in multi-site incorporation of GFP and VHH. The service supports pAcF and pAzF for preciseADC and provides custom nnAA testing with client-supplied components. With broad nnAA selection, no membrane or toxicity limits, proven orthogonal systems, and short turnaround, we deliver proteins with novel chemical, structural, and functional properties.

Insertion efficiency of unnatural amino acids