General Introduction of Stable Cell Lines Construction
Stable cell line construction involves cloning exogenous DNA into vectors carrying specific antibiotic resistance or selection genes. After transfection into host cells, selection markers on the vector are used to screen for cell lines capable of stably expressing the target protein.
Synbio Technologies provides a series of stable cell line construction services for the stable and large-scale production of recombinant proteins / antibodies such as full-length antibodies and antibody fragments, Fc fusion proteins, various cell surface receptors, cytoplasmic and secretory proteins.
Highlights
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- Full-process service from gene synthesis to screening
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- CHO1.0 stable transfection vector, combined with puromycin + MTX screening system
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- Efficient stable transfection and Pool screening
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- Customized expression of full-length or fragment recombinant proteins/antibodies
Service Details
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Service Steps
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Service Details
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Turnaround Time
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Gene Synthesis
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Full gene synthesis, provision of expression vector
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2-3 weeks
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Small-scale Expression, Analysis & Identification
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Plasmid small-scale extraction, identification, transfection of CHO cells, expression analysis, purification, activity and purity testing
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2 weeks
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Minipool Screening (>0.2g/L)
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1. Post-electroporation (pressured screening);
2. Well plate screening and expansion experimental data;
3. Minipool yield testing experimental data;
4. Fed-Batch (not less than 10 days) antibody expression 30ml for Top 12 candidate clones;
5. Top 3 candidate banking;
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7-12 weeks
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Monoclonal Screening
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1. Limited dilution monoclonal screening;
2. Yield testing;
3. Cell culture process experimental data;
4. Monoclonal screening process record data;
5. Fed-Batch (not less than 10 days) antibody expression 30ml for Top 25 monoclonal clones;
6. Top 3 candidate monoclonal banking;
7. Monoclonal expression level not less than 0.5g/L, three-generation stability;
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6-10 weeks
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Workflow
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