Monoclonal Antibody Preparation
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Monoclonal Antibody Preparation
Monoclonal antibody technology involves fusing a single B lymphocyte capable of producing antibodies with myeloma cells, resulting in hybrid cells that can generate both antibodies and proliferate indefinitely, thereby enhancing antibody production. This technology has been widely applied in fundamental research, medical diagnosis, and tumor-targeted therapy, among other fields.
Synbio Technologies has a robust antibody drug discovery platform utilized by our talented scientific team. Our platform provides a full range of services in custom monoclonal antibody production including immunization, hybridoma development, antibody discovery, antibody functional screening assays, antibody engineering, and antibody expression. Using this platform, we can deliver up to 10 high-quality functional hybridoma clones to jumpstart our customers’ antibody research.
Highlights
  • Proprietary Immunization Technology
    Universal Antigen can induce antibodies to recognize epitopes containing less than 5 aa
  • One-Stop Solution
    Streamlined strategy focuses on immunization, hybridoma generation, and characterization
  • High-Throughput Screening
    Immunization and high-throughput screening is optimized for affinity measurements, epitope binning, isotyping, etc.
  • Comprehensive Functional Assay
    Extensive experience held in antibody affinity ranking, affinity measurement, cross-reactivity, and other in-vitro biological analyses
Service Details
Milestones Details TAT Deliverable
Animal Immunization Blood collection before immunization 7-9 weeks Interim report (Including antiserum titer test results)
First immunization
(Antigen plus Freund's complete adjuvant)
Second immunization
(Antigen plus Freund's incomplete adjuvant)
Third immunization
(Antigen plus Freund's incomplete adjuvant)
Blood sampling and ELISA test
Fourth Immunization (Antigen is dissolved in PBS or saline)
Animal blood collection

Cell fusion and screening
Cell fusion and screening 5-6 weeks Interim report (Including screening data)
Subcloning and cell expansion
Cell line cryopreservation
Antibody production and purification Hybridoma cell culture 4-6 weeks 1-3mg monoclonal antibody with a titer of 100,000
Final experiment report
Antibody purification
ELISA, SDS-PAGE, concentration determination
FAQs
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Get in Touch with Us
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  • Address:
    9 Deer Park Dr., Suite J-25
    Monmouth Junction, NJ 08852
  • Tel: +1 732-230-3003
  • Fax: +1 609-228-5911
  • Inquiries: quote@synbio-tech.com

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