Standard Human CRISPR Libraries

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Synbio Technologies has launcheds its Syno Human CRISPR Knockout/Activation Library utilizing customized CRISPR library construction for targeted research applications. Our top-notch design and standardized library construction ensure high-quality results, lower research costs, and faster product delivery. High-quality human CRISPR libraries support research in gene function, drug development, molecular diagnostics, molecular breeding, and more.

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• Syno Human CRISPR Knockout Library

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Syno human genome-wide CRISPR knockout library consists of three independent sub-libraries: A, B, and C.

Sub-library A targets 18,913 protein-coding genes. It is ideal for high-quality screening and minimal sgRNA coverage.

Sub-library B expands this by 18,334 genes, providing additional sgRNAs for more expansive coverage.

Sub-library C, our compact and powerful library yet, targets 37,522 gene-specific and 200 non-targeted sgRNAs across 18,761 genes. This makes it particularly effective for intricate CRISPR screenings in cancer research and complex genetic studies.

Catalog	Product Name	Sequence Details	Vector Details	Specifications
STCRI211101	Syno Human Genome CRISPR Knockout Libraries	Sub-library A 18,913 genes are targeted, 74,552 sgRNA sequences and 435 control (non-targeted) sgRNA sequences are designed, with a total of 74,987 sgRNA sequences.	pLentiCRISPR v2 (with Puro mark)	200 μg
			pLentiGuide (with Puro mark)
			pLentiGuide (with Puro-GFP mark)
STCRI211102		Sub-Library B 18,334 genes are targeted, 71,048 sgRNA sequences and 435 control (non-targeted) sgRNA sequences are designed, with a total of 71,483 sgRNA sequences.	pLentiCRISPR v2 (with Puro mark)	200 μg
			pLentiGuide (with Puro mark)
			pLentiGuide (with Puro-GFP mark)
STCRI211103		Sub Library C 8,761 genes are targeted, 37,522 sgRNA sequences and 200 control (non-targeted) sgRNA sequences are designed, with a total of 37,722 sgRNA sequences.	pLentiCRISPR v2 (with Puro mark)	200 μg

• Syno Human Genome Knockout Negative Control Library

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The genome knockout negative control library serves as a negative control for the human genome CRISPR sgRNA library. It is suitable for experiments in human cells.

Catalog	Product Name	Sequence Details	Vector Details	Specifications
STCRI24301	Human Genome Knockout Negative Control Library	20 control (non-targeted) sgRNA sequences are designed, sgRNA size 20 bp.	pLentiCRISPR v2-Puro	150 μg
STCRI24302		50 control (non-targeted) sgRNA sequences are designed, sgRNA size 20 bp.	pLentiCRISPR v2-Puro	150 μg
STCRI24303		100 control (non-targeted) sgRNA sequences are designed, sgRNA size 20 bp.	pLentiCRISPR v2-Puro	150 μg
STCRI24304		200 control (non-targeted) sgRNA sequences are designed, sgRNA size 20 bp.	pLentiCRISPR v2-Puro	150 μg
STCRI24305		500 control (non-targeted) sgRNA sequences are designed, sgRNA size 20 bp.	pLentiCRISPR v2-Puro	150 μg
STCRI24306		1000 control (non-targeted) sgRNA sequences are designed, sgRNA size 20 bp.	pLentiCRISPR v2-Puro	150 μg
STCRI24307		2000 control (non-targeted) sgRNA sequences are designed, sgRNA size 20 bp.	pLentiCRISPR v2-Puro	150 μg

• Syno Human Genome CRISPR Activation Libraries

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Catalog	Product Name	Sequence Details	Vector Details	Specifications
STCRI233301	Human Genome CRISPR Activation Library	Sub-library A 18,885 genes are targeted, 56,266 sgRNA sequences and 496 control (non-targeted) sgRNA sequences are designed, sgRNA size 20 bp.	Syn-pXPR_502	200 μg
STCRI233302	Sub-library B 18,843 genes are targeted, 55,980 sgRNA sequences and 496 control (non-targeted) sgRNA sequences are designed, sgRNA size 20 bp.	Syn-pXPR_502	200 μg

• Syno Human Membrane Protein CRISPR Activation Library

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Catalog	Product Name	Sequence Details	Vector Details	Specifications
STCRI113345	Human Membrane Protein CRISPR Activation Library	6,213 genes are targeted, 58,071 sgRNA sequences and 500 control (non-targeted) sgRNA sequences were designed, totaling 58,571 sgRNA sequences.	pKVL2-U6gRNA_ SAM(BbsI)-PGKpuroBFP-W	200 μg

• Human Pathway sgRNA Library (Common Pathway)

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* Lentisyn005 Vector

Metabolic process	Catalog	Library name	Gene numbers
Common Pathway	SHPF231311	ABC transporter pathway	10021
	SHPF231312	Acute myeloid leukemia pathway	45
	SHPF231313	Adipogenesis pathway	126
	SHPF231314	Adult stem cell pathway	58
	SHPF231315	Angiogenesis pathway	92
	SHPF231316	Apoptosis pathway	68
	SHPF231317	cAMP&Ca pathway	91
	SHPF231318	Cell cycle pathway	84
	SHPF231319	Chemokine pathway	134
	SHPF231320	Drug Resistance pathway	348
	SHPF231321	ES differentiation pathway	334
	SHPF231322	GPCR pathway	280
	SHPF231323	Hormone activity pathway	108
	SHPF231324	Ion channel pathway	67
	SHPF231325	P450 pathway	57
	SHPF231326	Tumor metastasis pathway	60
	SHPF231327	Tumor suppressor pathway	720
	SHPF231328	Neelamegham human glycogene pathway	347
	SHPF231329	Human mTORC1 Focused pathway	713
	SHPF231330	Pan druggable library	75
	SHPF231331	Human interferon stimulated gene pathway	1914

• Human Pathway sgRNA Library (DNA replication and repair)

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Metabolic process	Catalog	Library name	Gene numbers
DNA replication and repair	SHPF231001	Base excision repair	33
SHPF231002	DNA replication	36
SHPF231003	Fanconi anemia pathway	54
SHPF231004	Homologous recombination	41
SHPF231005	Mismatch repair	23
SHPF231006	Non-homologous end-joining	13
SHPF231007	Nucleotide excision repair	47

• Human Pathway sgRNA Library (Transcription and Translation)

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Metabolic process	Catalog	Library name	Gene numbers
Transcription	SHPF232001	Basal transcription factors	45
SHPF232002	RNA polymerase	34
SHPF232003	Spliceosome	154
Translation	SHPF233001	Aminoacyl-tRNA biosynthesis	66
SHPF233002	mRNA surveillance pathway	97
SHPF233003	Nucleocytoplasmic pathway	108
SHPF233004	Ribosome biogenesis in eukaryotes	118
SHPF233005	Ribosome	167

• Human Pathway sgRNA Library (Transport and catabolism)

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Metabolic process	Catalog	Library name	Gene numbers
Transport and catalism	SHPF234001	Autophagy	141
SHPF234002	Endocytosis	251
SHPF234003	Lysosome	132
SHPF234004	Mitophagy	72
SHPF234005	Peroxisome	82
SHPF234006	Phagosome	152

• Human Pathway sgRNA Library (Cellular community, cell death, and cell motility)

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Metabolic process	Catalog	Library name	Gene numbers
Cellular community	SHPF235001	Adherens junction	71
SHPF235002	Focal adhesion	201
SHPF235003	Gap junction	88
SHPF235004	Signaling pathways regulating pluripotency of stem cells	143
SHPF235005	Tight junction	169
Cell death	SHPF236001	Apoptosis	136
SHPF236002	Cell cycle	127
SHPF236003	Cellular senescence	156
SHPF236004	Ferroptosis	41
SHPF236005	Necroptosis	159
SHPF236006	Oocyte meiosis	131
SHPF236007	p53 signaling pathway	73
Cell motility	SHPF236101	Regulation of actin cytoskeleton	229

• Human Pathway sgRNA Library (Folding/sorting and degradation)

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Metabolic process	Catalog	Library name	Gene numbers
Folding, sorting and degradation	SHPF237001	Proteasome	46
SHPF237002	Protein export	23
SHPF237003	Protein processing in endoplasmic reticulum	171
SHPF237004	RNA degradation	79
SHPF237005	SNARE interactions in vesicular transport	33
SHPF237006	Sulfur relay system	8
SHPF237007	Ubiquitin mediated proteolysis	142

• Human Pathway sgRNA Library (Development, regeneration, signal transduction)

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* Lentisyn005 Vector

Metabolic process	Catalog	Library name	Gene numbers
Development and regeneration	SHPF238001	Axon guidance	182
Development and regeneration	SHPF238002	Osteoclast differentiation	128
Signal transduction	SHPF239001	AMPK signaling pathway	121
	SHPF239002	Apelin signaling pathway	139
	SHPF239003	Calcium signaling pathway	240
	SHPF239004	cAMP signaling pathway	225
	SHPF239005	cGMP-PKG signaling pathway	167
	SHPF239006	ErbB signaling pathway	85
	SHPF239007	FoxO signaling pathway	131
	SHPF239008	He dgehog signaling pathway	56
	SHPF239009	HIF-1 signaling pathway	109
	SHPF239010	Hippo signaling pathway	157
	SHPF239011	JAK-STAT signaling pathway	166
	SHPF239012	MAPK signaling pathway	294
	SHPF239013	mTOR signaling pathway	156
	SHPF239014	NF-kappa B signaling pathway	104
	SHPF239015	Notch signaling pathway	59
	SHPF239016	Phosphatidylinositol signaling system	97
	SHPF239017	Phospholipase D signaling pathway	148
	SHPF239018	PI3K-Akt signaling pathway	354
	SHPF239019	Rap1 signaling pathway	210
	SHPF239020	Ras signaling pathway	236
	SHPF239021	Sphingolipid signaling pathway	119
	SHPF239022	TGF-beta signaling pathway	95
	SHPF239023	TNF signaling pathway	114
	SHPF239024	VEGF signaling pathway	59
	SHPF239025	Wnt signaling pathway	170

• Human Pathway sgRNA Library (Signaling molecules and interaction)

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Metabolic process	Catalog	Library name	Gene numbers
Signaling molecules and interaction	SHPF231011	Cell adhesion molecules	157
SHPF231012	Cytokine-cytokine receptor interaction	295
SHPF231013	ECM-receptor interaction	88
SHPF231014	Neuroactive ligand-receptor interaction	367
SHPF231015	Viral protein interaction with cytokine and cytokine receptor	100

• Human Pathway sgRNA Library (Circulatory system)

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Metabolic process	Catalog	Library name	Gene numbers
Circulatory system	SHPF231111	Adrenergic signaling in cardiomyocytes	154
SHPF231112	Cardiac muscle contraction	87
SHPF231113	Vascular smooth muscle contraction	134

• Human Pathway sgRNA Library (Immune system)

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* Lentisyn005 Vector

Metabolic process	Catalog	Library name	Gene numbers
Immune system	SHPF231211	Antigen processing and presentation	78
	SHPF231212	B cell receptor signaling pathway	82
	SHPF231213	Chenokine signaling pathway	192
	SHPF231214	Complement and coagulation cascades	86
	SHPF231215	C-type lectin receptor signaling pathway	104
	SHPF231216	Cytosolic DNA-sensing pathway	66
	SHPF231217	Fc epsilon RI signaling pathway	68
	SHPF231218	Fc ganna R-mediated phagocytosis	97
	SHPF231219	Hematopoietic cell lineage	99
	SHPF231220	IL-17 signaling pathway	94
	SHPF231221	Intestinal immune network for IgA production	49
	SHPF231222	Leukocyte transendothelial migration	114
	SHPF231223	Natural killer cell mediated cytotoxicity	132
	SHPF231224	Neutrophil extracellular trap formation	190
	SHPF231225	NOD-like receptor signaling pathway	186
	SHPF231226	Platelet activation	124
	SHPF231227	RIG-I-like receptor signaling pathway	71
	SHPF231228	T cell receptor signaling pathway	104
	SHPF231229	Th1 and Th2 cell differntiation	92
	SHPF231230	Th17 cell differentiation	108
	SHPF231231	Toll-like receptor signaling pathway	104

QUOTE

FAQs

Syno human whole genome library contains three sub-libraries, what is the difference between these three sub-libraries?

Sublibrary A contains the best-ranked sgRNAs according to the design criteria, enabling high-quality screening at lower library coverage.

Sublibrary B contains the second tier of sgRNAs and can be used to complement sublibrary A when higher sgRNA coverage is required.

Sublibrary C is smaller in size than any of the currently publicly available human genome-wide CRISPR libraries. It is mostly used for CRISPR screening of cancer cell models under complex conditions.

Which fields are Syno human genome CRISPR activation libraries are mainly used?

Activation libraries are whether genes are continuously expressed after being activated, and are used in gene function research, drug discovery, molecular diagnostics, drug target discovery and validation, and other fields.

What is the difference between human genome-wide library and human membrane protein library?

Genome-wide libraries include membrane protein libraries, so if you are doing research on membrane proteins, you can choose targeted membrane protein libraries, which are more efficient and convenient.

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