The General Introduction of Polyclonal Antibody Preparation
Polyclonal antibodies are a mixture of antibodies that recognize different epitopes of a specific antigen; these antibodies can be produced by collecting antiserums after artificially immunizing the host. Because they can recognize multiple epitopes, polyclonal antibodies have a higher affinity and sensitivity than monoclonal antibodies and are more tolerant to small antigen changes.
With rich experience in antigen design and mature
antigen/antibody preparation technology, Synbio Technologies provides customized services ranging from antigen design and preparation to gene synthesis to animal immunity to high throughput antibody production. In addition, various antigens, such as peptide antigens, protein antigens, and small molecule antigens, for several types of animals are available. Our technical team will evaluate your requirements and tailor your project to meet your needs—ultimately, we can deliver high quality polyclonal antibodies for your scientific research.
Highlights
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- High Titer
The titer of ELISA will be greater than 1:100000. For protein antigens, the delivered polyclonal antibodies will be positive for Western Blot
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- Highly Customized Services
Exclusive, personalized experimental schemes are provided to meet each customer's needs
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- One-Stop Solution
We offer a variety of services, from gene synthesis to antigen preparation to high quality antibody production
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- Excellent Communication
We will maintain frequent communication with customers, offering progress updates and issue reports throughout the entire project
Service Details
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Process
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Details
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Turnaround Time
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Deliverables
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Price
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Antigen Preparation
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Antigen provided by the customer.
1. 10 mg of peptide
2. 5-8 mg of recombinant protein of more than 85% purity
3. Small molecular antigens
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2-4 weeks
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QC report of antigen preparation
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Quote
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Synthesized or prepared by Synbio Technologies.
1. Design and synthesize polypeptide, coupled with KLH/BSA/OVA
2. Codon optimization, gene synthesis, recombinant protein expression and purification
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Animal Immunization
(mice,rabbits,goat)
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Immunize 3 ~ 4 times
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6~7 weeks
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Antibody Purification
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Antigen affinity purification or Protein A/G affinity purification
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1-2 weeks
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0.5-10 mg purified antibody,
ELISA, titer > 1:100000
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Quality Control
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Antibody purity test (SDS-PAGE);
specific detection of antigen and antibody (Western Blot analysis);
antibody titer detection (ELISA, titer > 1:100000)
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1-2 weeks
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QC report
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Additional Tests
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Antibody affinity test; antibody labeling;
identification of antibody subtypes
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Quote
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Quote
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Quote
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Workflow
FAQs
Immunodiagnostic, immunoprecipitation and other tests, especially when multiple antibody responses are required.
The antigen for antibody preparation is a prokaryotic recombinant protein, which is different from the natural protein samples to be used for WB, and it is not possible to guarantee that the conformation and activity are the same as that of the natural protein. There is no guarantee that the prepared antibody can be applied to the WB of natural protein, but it can be guaranteed that the prepared antibody will be positive to the WB of the antigen protein.
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