CpG ODNs|Synbio Technologies_Synbio Technologies

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General Introduction of CpG ODNs

CpG ODNs are short, synthetic, single-stranded DNA molecules containing unmethylated oligo deoxynucleotides (ODNs). Pre-clinical studies and trials have demonstrated that CpG ODNs can significantly improve vaccine-specific antibody responses.

CpG ODNs possess a partial or complete phosphorothioate (PS) backbone, as opposed to the natural phosphodiester (PO) backbone found in genomic bacterial DNA. Three major classes of stimulatory CpG ODNs have been identified. These classes are based on structural characteristics and activity on human peripheral blood mononuclear cells (PBMCs), in particular B cells and plasmacytoid dendritic cells (pDCs). These three classes are Class A (Type D), Class B (Type K) and Class C.

Synbio Technologies offer these three types of CpG ODNs. Each class boasts unique structural characteristics and immune effects suited for different applications.

Highlights

High Purity

＞95% purity
Low Endotoxin

<5 EU/mg endotoxin level
Various Unit Size

Sizing from mg to g
Cost-Effective

Competitive prices and Customized Synthesis

Service Details

Class A CpG ODNs are characterized by a PO central, CpG-containing palindromic motif and a PS-modified 3’ poly-G string. They induce high IFN-α production from pDCs but are weak stimulators of TLR9-dependent NF-κB signaling and pro-inflammatory cytokine (e.g. IL-6) production.

Class B CpG ODNs are linear and fully modified with phosphorothioates. They possess strong immunostimulatory activity on B cells but fail to activate plasmacytoid dendritic cells.

Class C CpG ODNs combine features of both class A and B, making them highly versatile as they activate both plasmacytoid dendritic cells and B cells.

Name	Cat（#）	Species	Size/Tube
CpG ODN 1585	SOC-A1585	Mouse	1mg
CpG ODN 2216	SOC-A2216	Human Preferred	1mg
CpG ODN 2336	SOC-A2336	Human Preferred	1mg
CpG ODN 1668	SOC-B1668	Mouse	1mg
CpG ODN 1826	SOC-B1826	Mouse	1mg
CpG ODN 2006/7909	SOC-B2006	Human Preferred	1mg
CpG ODN 2007	SOC-B2007	Bovine/Porcine	1mg
CpG ODN BW006/684	SOC-BW006	Human&Mouse	1mg
CpG ODN D-SL01	SOC-BDSL01	Multispecies	1mg
CpG ODN 2395	SOC-C2395	Human/Mouse	1mg
CpG ODN M362	SOC-CM362	Human/Mouse	1mg
CpG ODN D-SL03	SOC-CDSL03	Multispecies	1mg
CpG ODN 10101	SOC-10101	Human&Mouse	1mg
CpG ODN IMT504	SOC-IMT504	Human&Mouse	1mg
CpG ODN 1982	SOC-1982	Human&Mouse	1mg
CpG ODN 2088	SOC-2088	Human&Mouse	1mg
CpG ODN 21158	SOC-21158	Human&Mouse	1mg
CpG ODN 4084-F	SOC-4084-F	Human&Mouse	1mg
CpG ODN 5328	SOC-5328	Human&Mouse	1mg
CpG ODN BW001	SOC-BW001	Human&Mouse	1mg
CpG ODN INH-1	SOC-INH-1	Human&Mouse	1mg
CpG ODN M326	SOC-M326	Human&Mouse	1mg
CpG ODN TTAGGG	SOC-TTAGGG	Human&Mouse	1mg

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Deliverables

Lyophilized DNA Oligo Powder

Certificate of Analysis (COA)

Yield: 1mg

FAQs

Can 1mg of CpG be divided into 100ug per tube?

No, it can’t. By default, the shipping form is 1 mg per tube and cannot be divided. After receiving the samples, customers can dissolve and divide them into frozen aliquots, using one tube at a time.

Can CpG be modified, and what are the common modifications?

(1) Fluorescent modification, which can carry fluorescent groups (such as FAM, Cy3, FITC, etc.)

(2) Functional modifications, such as NH2 modification, THS modification, N3 modification, DBCO modification, etc., mainly for connection purposes, and GalNac mainly for liver targeting functions.

What are the storage and transportation conditions for CpG?

CpG is shipped at room temperature, and the dry powder can be stored at room temperature for several months. For long-term storage, keep at -20℃. After dissolution, it is recommended to prepare small aliquots and store the unused portions at -20℃ to avoid repeated freeze-thaw cycles.

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