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The General Introduction of Pathway Synthesis

Since the birth of synthetic biology in 2000, metabolic engineering and synthetic biology research have made increasingly huge progress on High-value-added natural products. The synthesis and construction of metabolic pathways as well as diverse DNA assembly technologies play fundamental roles in microbiology, biochemistry, and many other relevant fields.

Based on gene synthesis tools and strong, long DNA assembly capability, Synbio Technologies is confident in providing technical support for metabolic pathway synthesis and library assembly. Specific services include metabolic pathway synthesis and customized metabolic pathway library assembly. Our delivery of metabolic pathways and library plasmids can fully meet any customer’s needs.

Highlights

Enabling Swift Modular Gene Synthesis
Support for Diverse Sequences (Promoters, Ribozyme Sites, Terminators, etc.)
Facilitates Synthesis of up to 20kb Metabolic Pathways
Offers Flexible Library Assembly Methods

Service Details

Services	Details	Turnaround Time	Deliverables	Price
Metabolic Pathway Synthesis	Customized Metabolic Pathway Synthesis Services	<10Kb, 4-6 weeks; >10Kb, Inquire	Plasmids with synthesized metabolic pathway and glycerol bacteria	Inquire
Metabolic Pathway Library Assembly	Customized Metabolic Pathway Library Assembly Services	Varies depending on the pathway requested	Plasmid library after assembly and glycerol bacterial library, or monoclonal plasmids and glycerol bacteria.	Inquire

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Case Study

In this project, the variety of promoters used was key to achieving a diverse set of outcomes in the final constructed library. We aimed for a library with the capacity to produce 100 different variations, and by ensuring each variation was represented 100 times, we successfully created 10,000 individual clones. When we tested these clones using PCR, a staggering 78% of them showed the desired results, and every single one was confirmed to be accurate.

FAQs

What is the main purpose of metabolic pathway gene synthesis?

The main purpose of metabolic pathway gene synthesis is to construct and optimize gene networks by artificially synthesizing or editing gene sequences, thereby regulating the metabolic processes of organisms.

How long can the sequence length of metabolic pathway gene synthesis reach?

The synthetic length of a single metabolic pathway can reach up to 20kb. This length is sufficient to cover key genes and regulatory sequences in most metabolic pathways.

What are the application scenarios of metabolic pathway gene synthesis ?

Metabolic engineering : Improve the metabolic efficiency of organisms such as microorganisms and plants by synthesizing or optimizing metabolic pathway genes to produce more target products.

Synthetic biology : By synthesizing specific metabolic pathway genes or biological components, systems with specific functions can be constructed.

Biomedicine : By synthesizing disease-related metabolic pathway genes, we can gain insight into the pathological process of disease and explore new therapeutic targets.

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