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Synthetic CDR Variant Antibody Libraries

Synthetic antibody libraries are instrumental in designing, constructing, and successfully generating highly functional antibodies for various applications. These libraries feature optimized framework sequences (a robust scaffold) and controlled CDR diversity, giving them unique advantages over natural antibodies.

Synbio Technologies uses novel Trimer oligonucleotide-directed mutagenesis for codon optimization and synthesis of antibody CDR3 or all CDRs to create variant regions scFv or nanobody libraries. We have successfully delivered hundreds of high-quality, customized variant libraries. The antibody libraries, constructed with precise Trimer primers, provide technical support for antibody affinity maturation, drug target screening, specific antibody discovery, and other fields.

Highlights

Precise Targeting

Control codon preferences, amino acid composition, and proportion of each mutation site in the CDRs
High Diversity

High quality design leads to high diversity of the synthetic antibody libraries
High Quality Libraries

The updated phage display system provides guarantee for larger storage capacity and highly functional antibodies
Cost-Effective

Competitive prices and affordable services

Service Details

Process	Details	Turnaround Time
Sequence analysis and trimer oligos design	Optimize framework sequence and design the amino acid composition and proportion of the CDRs.	1-2 weeks
Vector construction	Sequence assembly and cloning in the antibody expression vectors.	2-3 weeks
Transform E.coli and Library construction	Transform the constructs into TG1 competent cells, then prepare the plasmid and identify the capacity and accuracy of the library.	2-3 weeks

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Workflow

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Deliverables

Synthesized variable fragment

Variable fragment phagemid library and COA

FAQs

What is the antibody library synthesis service?

The antibody library synthesis service is a biotechnology service aimed at constructing large-scale antibody libraries through synthetic methods. These libraries contain billions of antibody sequences, covering a wide range of antigen binding sites, and providing abundant resources for the screening and discovery of antibody drugs.

What are the keys to ensuring high diversity and quality of the antibody library ?

Design strategy: Adopting a reasonable antibody sequence design strategy to cover as many antigen binding sites as possible.

High-quality gene synthesis: Using advanced gene synthesis technology to ensure the accuracy of the synthesized DNA fragments.

Strict cloning and transformation conditions: Optimizing cloning and transformation conditions to improve transformation efficiency and library capacity.

Library quality control: Conducting rigorous quality control testing on the constructed library, including assessments of library capacity and diversity.

What are some applications of the antibody library synthesis service?

Drug development: Used for screening and discovering antibody drugs with therapeutic potential.

Diagnostic reagent development: Used for preparing diagnostic antibodies with high sensitivity and specificity.

Basic research: Providing abundant antibody resources for basic research in the field of life sciences.

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