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Oligo Synthesis

Oligonucleotide synthesis is the chemical process of creating short nucleic acid fragments with a defined sequence. This process involves synthesizing 2 ‘- deoxynucleosides (dA, dC, dG, dT), ribonucleosides (A, C, G, U), or chemically modified nucleosides (such as LNA) using the solid-phase phosphoramidite method. These fragments can be tailored for specific chemical structures and functions. Oligo synthesis is known for its stability, efficiency, and speed, and is commonly used in primers, probes, PCR amplification, DNA sequencing, molecular diagnostics, and other applications.

At Synbio Technologies, our advanced oligo synthesis platform is equipped with state-of-the-art synthesizers and supported by a team of experts in synthesis and modification technologies. This allows us to deliver high-quality oligonucleotide products to researchers and industrial customers worldwide. We offer a broad range of oligonucleotide synthesis options, including custom DNA oligos, diagnostic oligos & probes, NGS oligos, modified oligos, antisense oligos, and CpG ODNs. Additionally, we provide high-throughput oligo library and oligo pool synthesis for large-scale projects.

With over a decade of experience in synthetic biology, make Synbio Technologies your trusted partner for all your oligonucleotide synthesis needs.

Highlights of Oligo Synthesis

High-Quality Products

Certified with the highest manufacturing standards (ISO9001 and ISO13485), stringent QC & QA standards, large-scale sequencing verifying the average mutation rate to be lower than 1/1000
Highly-Customized

Various highly customizable options of purification, specifications and delivery forms, and flexible synthesis scale is available upon request all well
Multiple Application

Qualified and available for multiple applications such as molecular diagnosis, chip sequencing, genome engineering, tumor research, forensic identification, precision medicine, IVD, digital information storage, etc

Oligonucleotide Primers and Probes

The most common use of synthetic oligonucleotides is as relatively short probes and primers (typicallyup to 30-mer) in a variety of applications. This involves synthesizing a nucleotide sequence that is paired or 'reverse-complementary' to a larger, target DNA or RNA strand (target sequence).

As primers,oligos initiate enzymatic reactions by providing a starting point for DNA or RNA synthesis..
As probes, oligos bind specifically to complementary DNA or RNA sequences, enabling the detection or confirmation of target sequences in a sample..

Applications of Olihonucleotides

Therapeutic Development

Therapeutic Development Oligonucleotides (oligos) play a crucial role in therapeutic development, serving as the foundation for advanced treatments such as antisense therapies, RNA interference (RNAi), and mRNA-based vaccines. These molecules enable precise targeting of genetic sequences, allowing for modulation of gene expression to treat genetic disorders, cancers, and infectious diseases.

Genetic Engineering

In genetic engineering, oligos are indispensable tools for designing and manipulating DNA sequences. They are used in gene editing technologies like CRISPR-Cas9 as guide RNAs, in site-directed mutagenesis for creating specific genetic modifications, and in synthetic biology for assembling and optimizing novel genetic constructs.

Molecular Diagnostics

Oligos are essential in molecular diagnostics, enabling highly sensitive and specific detection of genetic material. They serve as primers and probes in PCR, qPCR, and next-generation sequencing (NGS) assays, facilitating the identification of pathogens, genetic mutations, and biomarkers for various diseases, including cancer and infectious diseases.

Case Study: Circular ssDNA Preparation and Emerging Applications

Unlike conventional linear DNA or double-stranded DNA (dsDNA), CssDNA is a covalently closed single-stranded DNA molecule with no free 5' or 3' ends. This unique topology provides distinct biochemical and biological advantages, making CssDNA an attractive platform for applications ranging from genome engineering to DNA vaccines.