Home > Oligo Synthesis > Modified Oligos

The General Introduction of Modified Oligos

Synbio Technologies provides high-quality oligo synthesis and modification. Our specialized services include oligonucleotides (which can be modified with special bases), dU/dI, phosphorothioates, phosphorylation, spacers, amino linkers, disulfide linkers, biotin, etc. In addition, Synbio Technologies also provides multiple fluorescent groups and quenchers at 3’, 5’, or designated sites specified by the customer. Some of these sites include FAM, HEX, ROX, BHQ, Texas Red, and others. The resulting oligos can be used in a variety of applications including PCR cloning, sequence verification, and gene detection.

Highlights

Comprehensive Modifications and Labeling

The average mutation rate is lower than 1/1000
Various Modification/Labeling

Modification/labeling type 200+
Strict Quality Control

Stringent ISO9001 and ISO13485 Certified Processing for oligo synthesis
Multiple Purification Options

Includes desalt, PAGE Plus, PAGE, RP-HPLC, IE-HPLC and Dual HPLC

Service Details

Synbio Technologies provides a series of general modifications with high purity and competitive prices. We accomplish this by focusing on requests of modification with special chemical classifications. Browse general modifications listed below:

Classification	Modifications	Purification
Modified Bases	dU	HPLC/PAGE
	dI
	5-Methyl dC
DBCO	5’ DBCO
	3’ DBCO
	dT-DBCO
AMCA	5’ AMCA
	3’ AMCA
	dT-AMCA
Phosphorothioates	SPO3
Amino Linkers	5’Amino C6
	dT-Amino C6
	3’Amino C7
	5’Amino C12
Biotin	5’Biotin
	3’Biotin
	dT-Biotin
Digoxigenin	5’Digoxigenin
	3’Digoxigenin
	dT-Digoxigenin
Phosphorylation	5’ PO4
Phosphorylation	3’ PO4
Hydrosulphonyl	5’ HS-C6
Hydrosulphonyl	3’ HS-C3
Locked Nucleic Acid	LNA (A/G/C/U)
Fluoro Bases	2’-F-2’-dA
	2’-F-2’-dC
	2’-F-2’-dG
	2’-F-2’-dU
Spacer	dSpacer
	Spacer C3
	Spacer C9
	Spacer C18

* The above is our suggested purification method. If you need other purification methods, please contact us for a quote.

Synbio Technologies provides multiple fluorescent groups at 3’, 5’ or designated sites to comply with our customers’ requests. This includes FAM, HEX, ROX, BHQ, Texas Red, etc.

Dye/Quenchers	3′-	5′-	Designated Sites	Purification
FAM	√	√	√	HPLC/PAGE
TET	√	√	√
JOE	√	√	√
HEX	√	√	√
SIMA	√	√	√
TAMRA	√	√	√
ROX	√	√	√
Cy5	√	√	√
Cy3	√	√	√
Quasar670	√	√
Quasar570	√	√
DyLight547		√
DyLight647		√
Texas Red		√
BHQ-1	√
BHQ-2	√
BHQ-3	√
Eclipse	√
DABCYL	√

* The above is our suggested purification method. If you need other purification methods, please contact us for a quote.

Dyes	Quenchers	Purification
5’6-FAM	3’TAMRA	HPLC/PAGE
3’BHQ-1
3’Eclipse
3’DABCYL
5’TET	3’TAMRA
3’BHQ-2
3’DABCYL
5’HEX	3’TAMRA
3’BHQ-1
3’BHQ-2
3’DABCYL
5’TAMRA	3’Eclipse
3’BHQ-1
3’BHQ-2
3’DABCYL
5’ROX	3’Eclipse
3’BHQ-1
3’BHQ-2
3’DABCYL
5’CY5	3’BHQ-1
3’BHQ-2
3’BHQ-3
3’DABCYL
5’CY3	3’BHQ-2
5’JOE	3’Eclipse
3’BHQ-1
3’BHQ-2
3’DABCYL
3’TAMRA
5’Texas Red	3’BHQ-2

QUOTE

Deliverables

Lyophilized dry DNA oligo powder, 1 tube (Transparent or dark) or 96-well and 384-well plates.

Certificate of Analysis (COA): including sequence information, OD, Tm, etc.

FAQs

How many types of modifier Oligos are there?

There are more than 300 types of modifier oligos.

Why are modified oligos less productive and more expensive than standard oligos?

The main reason is that the modified monomer is less stable, the coupling time is long, the efficiency is low, and the yield obtained at the end is naturally lower than the general oligos.
Modified oligos usually need to be purified by PAGE or HPLC and the purification process is difficult. Alongside this, you will need many more raw materials (on the order of 100x more.) Both of these factors cause modified oligos to be naturally more expensive.

Do modifier labels have an effect on OD measurements?

Some fluorescent groups absorb light at 260 nm, e.g. FAM, HEX, TAMRA, TET. Others may have absorption values at 260 nm, but the data is not published/verified and has therefore not been included in the calculation.

More FAQs>>

Resources