General Introduction of Modified Oligos
Synbio Technologies provides high-quality oligo synthesis and modification. Our specialized services include oligonucleotides (which can be modified with special bases)
, dU/dI, phosphorothioates, phosphorylation, spacers, amino linkers, disulfide linkers, biotin, etc. In addition, Synbio Technologies also provides multiple fluorescent groups and quenchers at 3’, 5’, or designated sites specified by the customer. Some of these sites include FAM, HEX, ROX, BHQ, Texas Red, and others. The resulting oligos can be used in a variety of applications including
PCR cloning, sequence verification, and gene detection.
Highlights
-
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- Comprehensive Modifications and Labeling
The average mutation rate is lower than 1/1000
-
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- Various Modification/Labeling
Modification/labeling type 200+
-
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- Strict Quality Control
Stringent ISO9001 and ISO13485 Certified Processing for oligo synthesis
-
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- Multiple Purification Options
Includes desalt, PAGE Plus, PAGE, RP-HPLC, IE-HPLC and Dual HPLC
Service Details
Synbio Technologies offers a comprehensive range of primer modification types, including single fluorescent labeling at the 5′ end, 3′ end, or any specified position, as well as dual-labeled probes; fluorophores and quenchers can be flexibly combined according to customer requirements, as detailed below:
|
Modification
|
5'Modification
|
Internal
|
3'Modification
|
|
dU
|
√
|
√
|
√
|
|
dI
|
√
|
√
|
√
|
|
Methyl dC
|
√
|
√
|
√
|
|
DBCO
|
√
|
√
|
√
|
|
DBCO PEG4
|
√
|
√
|
√
|
|
N3
|
√
|
√
|
√
|
|
TZ
|
√
|
√
|
√
|
|
TCO
|
√
|
√
|
√
|
|
COOH
|
√
|
|
√
|
|
CHO
|
√
|
|
√
|
|
Hexynly
|
√
|
|
|
|
`Acrydite
|
√
|
|
|
|
AMCA
|
|
√
|
√
|
|
S
|
|
√
|
|
|
Amino C3
|
√
|
|
|
|
Amino C4
|
√
|
|
|
|
Amino C5
|
√
|
|
|
|
Amino C6
|
√
|
|
|
|
Amino C7
|
|
|
√
|
|
UniAmM
|
|
√
|
|
|
Amino C12
|
√
|
|
|
|
Biotin
|
√
|
√
|
√
|
|
Biotin TEG
|
√
|
|
√
|
|
Digoxigenin
|
√
|
√
|
√
|
|
GalNac
|
√
|
|
√
|
|
Cholesterol
|
√
|
|
√
|
|
Cholesterol TEG
|
√
|
|
√
|
|
Ferrocene
|
√
|
|
|
|
AQ (Anthraquinone)
|
√
|
|
√
|
|
TPP
|
√
|
|
√
|
|
PHO
|
√
|
|
√
|
|
NOTA
|
√
|
|
√
|
|
DOTA
|
√
|
|
√
|
|
puromycin
|
|
|
√
|
|
i3Cyan (CNVK)
|
|
√
|
|
|
Dinitrophenol (DNP)
|
√
|
|
|
|
Thiol C6 S-S
|
√
|
|
√
|
|
Thiol C3 S-S
|
|
|
√
|
|
dSpacer
|
|
√
|
|
|
Spacer C3
|
√
|
√
|
√
|
|
Spacer 6
|
√
|
√
|
√
|
|
Spacer 9
|
√
|
√
|
√
|
|
Spacer C12
|
√
|
√
|
√
|
|
Spacer 18
|
√
|
√
|
√
|
|
Stearyl (C18)
|
√
|
|
|
|
Hexadecane (C16)
|
√
|
|
√
|
|
Acrydite
|
√
|
|
|
|
PC Amino
|
√
|
|
|
|
PC Biotin
|
√
|
|
|
|
PC Spacer C3
|
|
√
|
|
|
FAM
|
√
|
√
|
√
|
|
TET
|
√
|
|
√
|
|
JOE
|
√
|
|
√
|
|
VIC
|
√
|
√
|
√
|
|
TAMRA
|
√
|
√
|
√
|
|
NED
|
√
|
√
|
√
|
|
PET
|
√
|
√
|
√
|
|
ROX
|
√
|
√
|
√
|
|
Cy5
|
√
|
√
|
√
|
|
Cy5.5
|
√
|
|
√
|
|
Cy7
|
√
|
|
√
|
|
Cy3
|
√
|
√
|
√
|
|
Cy3.5
|
√
|
√
|
√
|
|
ATTO 488
|
√
|
|
√
|
|
ATTO 550
|
√
|
|
√
|
|
ATTO 565
|
√
|
|
√
|
|
ATTO 633
|
√
|
|
√
|
|
ATTO 647
|
√
|
|
√
|
|
Quasar670
|
√
|
√
|
√
|
|
Quasar570
|
√
|
√
|
√
|
|
Texas Red
|
√
|
√
|
√
|
|
BHQ1
|
√
|
√
|
√
|
|
BHQ plus
|
|
|
√
|
|
BHQ2
|
√
|
√
|
√
|
|
BHQ3
|
|
|
√
|
|
MGB
|
|
|
√
|
|
ZEN
|
|
√
|
√
|
|
TAO
|
|
√
|
√
|
|
Eclipse
|
|
|
√
|
|
DABCYL
|
|
|
√
|
Deliverables
-
Lyophilized dry DNA oligo powder, 1 tube (Transparent or dark) or 96-well and 384-well plates.
-
Certificate of Analysis (COA): including sequence information, OD, Tm, etc.
FAQs
|
Fluorescent group
|
Maximum excitation wavelength
|
Maximum emission wavelength
|
Emission color*
|
|
AMCA
|
353nm
|
422nm
|
Blue
|
|
FAM
|
494nm
|
518nm
|
Green
|
|
TET
|
521nm
|
538nm
|
Green-Yellow
|
|
JOE
|
520nm
|
548nm
|
Green-Yellow
|
|
HEX
|
535nm
|
553nm
|
Yellow
|
|
VIC
|
538nm
|
554nm
|
Yellow
|
|
CY3
|
552nm
|
570nm
|
Orange
|
|
NED
|
546nm
|
575nm
|
Orange
|
|
Rhodamine Green
|
560 nm
|
580 nm
|
Orange
|
|
Rhodamine Red
|
560 nm
|
580 nm
|
Orange
|
|
TAMRA
|
560nm
|
582nm
|
Orange-Red
|
|
TAMRA (amidite, for STR)
|
560nm
|
582nm
|
Orange-Red
|
|
PET
|
556nm
|
595nm
|
Orange/Red
|
|
Texas Red-X
|
583nm
|
603nm
|
Orange/Red
|
|
ROX
|
587nm
|
607nm
|
Orange/Red
|
|
CY5
|
649nm
|
662nm
|
Red
|
Additionally, SIMA has the same color as HEX, and FITC has the same color as FAM.
|
Fluorescent group
|
Maximum excitation wavelength
|
Maximum emission wavelength
|
Emission color
|
Quencher groups available
|
|
BHQ1
|
BHQ2
|
BHQ3
|
QSY7
|
DABCYL
|
TAMRA
|
|
480 - 580 (535)
|
550 - 650 (579)
|
620 - 730 (672)
|
500 - 600 (580)
|
380 - 550 (453)
|
470 - 560 (544)
|
|
AMCA
|
353nm
|
422nm
|
Blue
|
√
|
√
|
√
|
√
|
√
|
√
|
|
FAM
|
494nm
|
518nm
|
Green
|
√
|
√
|
√
|
√
|
√
|
√
|
|
TET
|
521nm
|
538nm
|
Green-Yellow
|
√
|
√
|
√
|
√
|
√
|
√
|
|
JOE
|
520nm
|
548nm
|
Green-Yellow
|
√
|
√
|
√
|
√
|
√
|
√
|
|
HEX
|
535nm
|
553nm
|
Yellow
|
√
|
√
|
√
|
√
|
√
|
√
|
|
VIC
|
538nm
|
554nm
|
Yellow
|
√
|
√
|
√
|
√
|
√
|
√
|
|
CY3
|
552nm
|
570nm
|
Orange
|
√
|
√
|
√
|
√
|
√
|
√
|
|
NED
|
546nm
|
575nm
|
Orange
|
|
√
|
|
√
|
|
|
|
Rhodamine Green
|
560 nm
|
580 nm
|
Orange
|
|
√
|
|
√
|
|
|
|
Rhodamine Red
|
560 nm
|
580 nm
|
Orange
|
|
√
|
|
√
|
|
|
|
TAMRA
|
560nm
|
582nm
|
Orange-Red
|
|
√
|
|
√
|
|
|
|
TAMRA (amidite, for STR)
|
560nm
|
582nm
|
Orange-Red
|
|
|
|
√
|
|
|
|
PET
|
556nm
|
595nm
|
Orange/Red
|
|
√
|
|
√
|
|
|
|
Texas Red-X
|
583nm
|
603nm
|
Orange/Red
|
|
√
|
|
√
|
|
|
|
ROX
|
587nm
|
607nm
|
Orange/Red
|
|
√
|
|
√
|
|
|
|
CY5
|
649nm
|
662nm
|
Red
|
|
√
|
√
|
|
|
|
A: FAM, VIC, biotin, CY5, TXR, etc..
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