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Precision Gene Editing
Customized Libraries and Complete Solutions for Gene Function Screening
Quote
Home > Precision Gene Editing
Precision Gene Editing
Synbio Technologies offers comprehensive precision gene editing services, including sgRNA design, oligo pool synthesis, CRISPR and sgRNA library construction, NGS validation, viral packaging, library cell pool creation, high-throughput screening, and bioinformatics analysis. We have successfully delivered hundreds of high-quality CRISPR sgRNA libraries for over 30 species of plants, animals, and microorganisms. Our tailored solutions meet diverse customization needs and provide a complete approach to gene function screening.
Highlights
  • 30+ Species, Customized sgRNA Design
  • Abundant sgRNA Expression Vectors, Provides Transfection-grade Plasmids
  • >99% Coverage, <1.6-10 Uniformity
  • One-stop Service from sgRNA Design, to Library Construction, to Lentiviral Packaging
Service Details
Service Specifications Service Steps Turnaround Time
sgRNA Design sgRNA sequences designed for specific genes.
Each genedesigned with 3-6 sgRNAs. 		1-2 weeks
Oligo Pool Synthesis All designed sgRNAs and negative controls synthesized on one chip 3-4 weeks
sgRNA Library Construction Cloning these sgRNAs on the target vector 2-3 weeks
Plasmid Purification Transfection-grade plasmid preparation to meet shipping needs 1 week
Library Quality Evaluation&NGS Verification(Optional) 1.100x greater library coverage
2.>70% cloning accuracy rate
3.>80% NGS verified library accuracy rate
4.<10 library uniformity index 		2-3 weeks
Viral Packaging（optional） Lentiviral packaging, viral titer: 108TU/ml 2-3 weeks
Library cell pool construction 1.Resistance optimal screening concentration Test
2.Lentiviral load and cell count evaluation
3.Resistance screening
4.NGS detection 		6-9 weeks
High-throughput screening PositiveScreeningExperiment,NegativeScreeningExperiment,
Drugresistanceassay,reportergeneassay 		2-3 weeks
QUOTE
How to Order?

 
Case Study

Screening resistance target genesfor a drug with identified function

Methods

1.The function and lethal dose of the drug have already been identified.

2.The CRISPR library was synthesized and transfected into cells using lentivirus vector.

3.Screen the drug of high concentration acting on the mixed cell clone continuously over a short period. Then rescreen the surviving cells. These cells are considered to be resistant to this drug. The reason why these cells gain a resistance to this drug is due to mutagenesis.

4.Perform NGS and bioinformatics analysis on the surviving cells, analyze information about the gRNA, and then identify the genes involved in conferring drug resistance.

 


Results

1.Identification of 6 genes involved in PLX resistant when mutant

2.Identification of NF2 as the resistant target of PLX in melanoma

FAQs
What are the areas of application of gene editing technology？

    Diagnostic reagent development, construction of disease models,and drug discovery,etc.

What are the classifications of methods in gene editing?

    The classification of methods in gene editing is: 

    1. Homologous recombination 

    2. Nucleases

What are the species involved in the editing systems we have constructed so far? According to the purpose of the experiment, how can this be catigorized?

    Some species that are currently well established in modern gene editing systems are E.coli, Saccharomyces cerevisiae, and Pichia pastoris.


    According to the purpose of the experiment, these can be categorized as such: gene knock-in, gene knock-out, and point mutation.

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