CRISPR Libraries
CRISPR libraries are widely used in high-throughput gene and genome function screening. Focusing on CRISPR
gene editing technology, Synbio Technologies not only provides sgRNA library synthesis services, but also customized services from high-throughput
sgRNA library construction, viral packaging, cell transfection, drug screening, NGS sequencing and data analysis. Meanwhile, we are highly experienced in sgRNA library designing, building, screening and various off-the-shelf libraries to choose from, such as CRISPR knockout,CRISPRa,and CRISPRi.
With over a decade of experience in sgRNA library synthesis, Synbio Technologies offers precise, efficient, and customized solutions to customers worldwide. Our comprehensive services include
sgRNA design,
oligo pool synthesis, CRISPR library/sgRNA library construction, NGS validation, and high-content screening, ensuring the highest standards of quality and innovation.
Highlights of CRISPR Libraries Service
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- Support Precision Editing with Minimal Off-target
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- One Stop Solution from Genome Wide sgRNA Design to Libraries Construction
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- Ensure Construction of Any Type of Libraries for Any Species
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- Delivery of Transfection Grade Plasmids
Services Details
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Service Specifications
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Service Steps
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Turnaround Time
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sgRNA Design
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sgRNA sequences designed for specific genes
Each gene designed with 3-6 sgRNAs
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1-2 weeks
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Oligo Pool Synthesis
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All designed sgRNAs and negative controls synthesized simultaneously
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3-4 weeks
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sgRNA Library Construction
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Cloning onto target vector
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2-3 weeks
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Plasmid Purification
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Transfection-grade plasmid preparation
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1 week
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Library Quality
NGS Verification(Optional)
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1.100x greater library coverage
2.>70% Cloning accuracy rate
3.>99% NGS verified Library accuracy rate
4.<10 Library uniformity index
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2-3 weeks
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Total
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9-13 weeks
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Workflow
Service Details
DNA Synthesis
Vector Selection
Molecular Biology
Oligo Synthesis
RNA Synthesis
Variant Libraries
CRISPR Libraries
Oligo Pools
Virus Packaging
Gene Editing
Protein Expression
Antibody Services
Peptide Services
DNA Data Storage
Standard Oligo
Standard CRISPR Libraries
Standard CRISPR Plasmid
ProXpress
Protein Products
