Plasmid DNA preparation is a process of separating and purifying circular double-stranded DNA molecules (i.e., plasmids) from bacteria (usually Escherichia coli) that contain plasmids. As small DNA fragments independent of chromosomal DNA and capable of autonomous replication in host cells, plasmids often carry genes such as antibiotic resistance genes and fluorescent protein genes, which endow host cells with specific biological characteristics. It is extremely useful in molecular biology research and biotechnology fields. Its main purpose is to provide materials for subsequent experiments such as cloning, sequencing, or serving as a vector for transforming other cells.
Synbio Technologies is dedicated to providing our customers with high-quality and customized plasmid DNA preparation services. We conduct comprehensive quality control of the plasmid products to provide you with aseptic plasmids containing no RNA or genome pollution. All plasmids we manufacture are free of animal-derived materials and can contain low levels of endotoxin (< 100 EU/mg, <30 EU/mg, <5 EU/mg, on request). We provide both research grade and transfection grade plasmids according to our customers needs. Order your plasmid DNA now and experience something brand new right away!
Size | TAT | Endotoxin Level | Price | |
---|---|---|---|---|
Research Grade | 100-200 μg | 5-8 BD |
< 0.1 EU/μg <0.05 EU/μg |
Quote |
200-500 μg | 5-8 BD | |||
500-1000 μg | 5-8 BD | |||
HT Grade | 15 μg | 5-8 BD | ||
GMP-Like Grade | 1-10 mg | 6-9 BD | ||
10-50 mg | 9-13 BD | |||
50 mg-1 g | Quote | |||
GMP Grade | 100 mg-10 g | Quote | < 0.01 EU/μg |
C Items* | Method | Specifications |
Default QC Research Grade |
Default QC Transfection Grade |
QC Price |
---|---|---|---|---|---|
Appearance | Visual inspection | Clear, colorless, free from visible particulates. | √ | √ | Free |
A 260/280 Ratio | UV Absorbance | 1.80 ~ 2.00. | √ | √ | Free |
Quantity | UV Absorbance | Quantity is ± 5%. | √ | √ | Free |
Residual RNA | Agarose gel electrophoresis | Not visible | √ | √ | Free |
Genomic DNA | Agarose gel electrophoresis | Not visible | √ | √ | Free |
Fragment Size | Restriction Digestion | The size of plasmids fragment is right and free of any contaminated bands. | √ | √ | Free |
Endotoxin control | TAL assay | < 100 EU/mg | x | √ | Free |
Supercoil content | Agarose gel electrophoresis | Supercoil content >90% | x | √ | Free |
Bioburden | Streak inoculation | No growth after 48 hours. | x | √ | Free |
Mycoplasma | Streak inoculation | No mycoplasma is detected in the plasmid products. | x | x | Quote |
Sequencing | Whole plasmids sequencing | Sequencing results are consistent with the confirmed plasmid sequence. | x | x | Quote |
Plasmid DNA plays an important role in various scientific and industrial fields and is an essential tool for advances in gene therapy, diagnostics and drug development.
The major users of plasmid DNA services include researchers, R&D managers and professionals in the fields of gene therapy, in vitro diagnostics, biopharmaceuticals, industrial biotechnology and academic research.
Whether for gene therapy diagnostics, vaccine research or drug screening, plasmid DNA remains an indispensable tool for advancing medicine and science.
When it comes to plasmid DNA, proper handling is key to maintaining its integrity. Here’s how to best manage shipping and storage:
Plasmid DNA is typically dispatched in a 1x TE buffer solution. This method allows for safe transportation at room temperature conditions, ensuring that the plasmid remains stable throughout its journey.
Temperature: For brief storage periods, up to two weeks, keep the plasmid at 4°C. This ensures the DNA remains stable without significantly impacting its functionality.
Temperature Options: For extended preservation, choose to store plasmids at either -20°C or -80°C. Both temperatures offer excellent long-term protection against degradation.
Important Tip
Avoid Freeze-Thaw Cycles: Repeated freeze-thaw actions can degrade plasmid DNA. Try to minimize these cycles to retain the plasmid's quality. By adhering to these recommendations, you ensure that your plasmid DNA remains viable and ready for your next experiment or application.
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