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Plasmid DNA Preparation

Plasmid DNA preparation is a process of separating and purifying circular double-stranded DNA molecules (i.e., plasmids) from bacteria (usually Escherichia coli) that contain plasmids. As small DNA fragments independent of chromosomal DNA and capable of autonomous replication in host cells, plasmids often carry genes such as antibiotic resistance genes and fluorescent protein genes, which endow host cells with specific biological characteristics. It is extremely useful in molecular biology research and biotechnology fields. Its main purpose is to provide materials for subsequent experiments such as cloning, sequencing, or serving as a vector for transforming other cells.

Synbio Technologies is dedicated to providing our customers with high-quality and customized plasmid DNA preparation services. We conduct comprehensive quality control of the plasmid products to provide you with aseptic plasmids containing no RNA or genome pollution. All plasmids we manufacture are free of animal-derived materials and can contain low levels of endotoxin (< 100 EU/mg, <30 EU/mg, <5 EU/mg, on request). We provide both research grade and transfection grade plasmids according to our customers needs. Order your plasmid DNA now and experience something brand new right away!

Highlights

Fast Turnaround Time

As fast as 2 BDs
Guaranteed Plasmid Preparation Services

USA-based manufacturing ensures high quality products
Highly Customized

Microgram-to-gram-scale quantity can meet various customer needs
Low Endotoxin Level

Animal-derived materials free, <100 EU/mg, on request
Strict Quality Control

ISO9001 & ISO13485 quality management, complete and detailed manufacturing documentation
One-stop Service

We provide a one-stop solution from gene synthesis to plasmid production

Service Details

Service Specifications

Size		TAT	Endotoxin Level	Price
Research Grade	100-200 μg	5-8 BD	< 0.1 EU/μg <0.05 EU/μg	Quote
	200-500 μg	5-8 BD
	500-1000 μg	5-8 BD
HT Grade	15 μg	5-8 BD
GMP-Like Grade	1-10 mg	6-9 BD
	10-50 mg	9-13 BD
	50 mg-1 g	Quote
GMP Grade	100 mg-10 g	Quote	< 0.01 EU/μg

Quality Control

C Items*	Method	Specifications	Default QC Research Grade	Default QC Transfection Grade	QC Price
Appearance	Visual inspection	Clear, colorless, free from visible particulates.	√	√	Free
A 260/280 Ratio	UV Absorbance	1.80 ~ 2.00.	√	√	Free
Quantity	UV Absorbance	Quantity is ± 5%.	√	√	Free
Residual RNA	Agarose gel electrophoresis	Not visible	√	√	Free
Genomic DNA	Agarose gel electrophoresis	Not visible	√	√	Free
Fragment Size	Restriction Digestion	The size of plasmids fragment is right and free of any contaminated bands.	√	√	Free
Endotoxin control	TAL assay	< 100 EU/mg	x	√	Free
Supercoil content	Agarose gel electrophoresis	Supercoil content >90%	x	√	Free
Bioburden	Streak inoculation	No growth after 48 hours.	x	√	Free
Mycoplasma	Streak inoculation	No mycoplasma is detected in the plasmid products.	x	x	Quote
Sequencing	Whole plasmids sequencing	Sequencing results are consistent with the confirmed plasmid sequence.	x	x	Quote

*Contact us for more QC items.

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Workflow

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Deliverables

Plasmid DNA (Dry powder/liquid)

COA file

Endotoxin test report (Optional)

Restriction enzyme digestion analysis (Optional)

Sequencing verification (Optional)

FAQs

What should I do if the plasmid copy number is low?

You can increase the volume of bacterial solution extracted and reduce the volume during elution, or replace it with a high-copy-number vector with the same function.

What are the downstream applications of plasmid DNA?

Our services cater to a wide range of applications, including gene function research, protein production, gene therapy, vaccine development, drug screening, and more.

Can you provide transfection level plasmid DNA?

We offer standard plasmid DNA preparation services for both research grade and transfection grade according to our customers' needs.

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