Plasmid DNA Preparation
High Quality, High Customization
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The General Introduction of Plasmid DNA Preparation

Plasmid DNA preparation is a process of separating and purifying circular double-stranded DNA molecules (i.e., plasmids) from bacteria (usually Escherichia coli) that contain plasmids. As small DNA fragments independent of chromosomal DNA and capable of autonomous replication in host cells, plasmids often carry genes such as antibiotic resistance genes and fluorescent protein genes, which endow host cells with specific biological characteristics. It is extremely useful in molecular biology research and biotechnology fields. Its main purpose is to provide materials for subsequent experiments such as cloning, sequencing, or serving as a vector for transforming other cells.


Synbio Technologies is dedicated to providing our customers with high-quality and customized plasmid DNA preparation services. We conduct comprehensive quality control of the plasmid products to provide you with aseptic plasmids containing no RNA or genome pollution. All plasmids we manufacture are free of animal-derived materials and can contain low levels of endotoxin (< 100 EU/mg, <30 EU/mg, <5 EU/mg, on request). We provide both research grade and transfection grade plasmids according to our customers needs. Order your plasmid DNA now and experience something brand new right away!

Highlights
  • Fast Turnaround Time
    As fast as 2 BDs
  • Guaranteed Plasmid Preparation Services
    USA-based manufacturing ensures high quality products
  • Highly Customized
    Microgram-to-gram-scale quantity can meet various customer needs
  • Low Endotoxin Level
    Animal-derived materials free, <100 EU/mg, on request
  • Strict Quality Control
    ISO9001 & ISO13485 quality management, complete and detailed manufacturing documentation
  • One-stop Service
    We provide a one-stop solution from gene synthesis to plasmid production
Service Details

Service Specifications

Size TAT Endotoxin Level Price
Research Grade 100-200 μg 5-8 BD < 0.1 EU/μg
<0.05 EU/μg
Quote
200-500 μg 5-8 BD
500-1000 μg 5-8 BD
HT Grade 15 μg 5-8 BD
GMP-Like Grade 1-10 mg 6-9 BD
10-50 mg 9-13 BD
50 mg-1 g Quote
GMP Grade 100 mg-10 g Quote < 0.01 EU/μg



Quality Control

C Items* Method Specifications Default QC
Research Grade
Default QC
Transfection Grade
QC Price
Appearance Visual inspection Clear, colorless, free from visible particulates. Free
A 260/280 Ratio UV Absorbance 1.80 ~ 2.00. Free
Quantity UV Absorbance Quantity is ± 5%. Free
Residual RNA Agarose gel electrophoresis Not visible Free
Genomic DNA Agarose gel electrophoresis Not visible Free
Fragment Size Restriction Digestion The size of plasmids fragment is right and free of any contaminated bands. Free
Endotoxin control TAL assay < 100 EU/mg x Free
Supercoil content Agarose gel electrophoresis Supercoil content >90% x Free
Bioburden Streak inoculation No growth after 48 hours. x Free
Mycoplasma Streak inoculation No mycoplasma is detected in the plasmid products. x x Quote
Sequencing Whole plasmids sequencing Sequencing results are consistent with the confirmed plasmid sequence. x x Quote
*Contact us for more QC items.
Workflow

Deliverables
Plasmid DNA (Dry powder/liquid)
COA file
Endotoxin test report (Optional)
Restriction enzyme digestion analysis (Optional)
Sequencing verification (Optional)
FAQs
Resources
Get in Touch with Us
Related Services
  • Promo Code: PLASMIDSAVINGS
    Promotion Duration: December 1 - December 31, 2024
    Contact Us
  • Address:
    9 Deer Park Dr., Suite J-25
    Monmouth Junction, NJ 08852

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