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Site-Directed Mutagenesis

Site-directed mutagenesis is a commonly utilized genetic research tool that effectively alters the properties and characteristics of target proteins. This service has widespread applications in studying protein functional sites, optimization of enzymatic activity, exploration of DNA component functions, and more.

Synbio Technologies offers efficient services for site-directed mutagenesis of genes. By leveraging PCR, we precisely modify the sequence of the target gene, including base insertions, deletions, and point mutations, to swiftly investigate the properties and functions of the target protein. Simply provide us with the sequence information of your target gene and the desired mutation sites, and we can achieve mutations at any site, starting from $75/mutant.

Highlights

Rapid TAT

Delivery within a minimum of 5 working days
Extensive Operating Region

Mutations within a 40 bp region are consolidated into a single mutation
Capability

Synthesis of sequences up to 200 kb in length
One Stop Solution

From gene synthesis, vector selection to protein expression and purification
Most Competitive Prices

starting at $75/mutant

Service Details

Cloned Fragment Length	Amount of Point Mutation	TAT (Business Days)	Price
< 2 kb	1	10	Starting from $ 75/mutant
	2
	3
2 kb-3 kb	1	13
	2
	3
3 kb-5 kb	1	16
	2
	3

QUOTE

Deliverables

1 tube of 2~5 µg dry lyophilized DNA

1 tube of glycerol bacteria or bacteria stab containing the corresponding plasmids (Additional fees apply)

Original peak diagram of sequencing results in .ab1 format

COA file, including QC enzyme digestion verification figure

FAQs

What are the downstream applications of point mutation services?

Site-directed mutagenesis technology is widely used in the study of protein functional site structures, optimization of enzyme activity, DNA element function or element interactions, gene therapy, etc.

Can mutations be introduced at any site?

Yes, our site-directed mutagenesis service can introduce mutations at any site in the plasmid.

What materials and information do I need to provide for site-directed mutagenesis service?

• High-purity Plasmid: Our lab requires a minimum of 4 μg of plasmid with a clear electrophoresis pattern, meeting the requirements for enzyme digestion and sequencing.

• Original Gene Sequence: Including the customer's full-length original template sequence. Additional required information includes the size, resistance, and other characteristics of the template plasmid.

• Target Mutation Sequence: Clearly indicate the base position(s) to be mutated and the sequence after mutation.

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