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Oligonucleotide Diagnostics & Therapeutics

Since the outbreak of COVID-19, Synbio Technologies has established a dedicated emergency project team, working tirelessly to produce virus-related probes, genes, and protein products. We currently provide comprehensive pathogen detection solutions such as diagnostic probes design, diagnostic reagent, and vaccine development.

Highlights

ISO 13485 Standards of Manufacturing, WHO/US/CN CDC Standards
Low False Positive Rate, Thus More Cost-effective
Expert Production Capacity
Guaranteed Swift Delivery

Detection Strategy

Synbio Technologies has successfully designed and synthesized detection probes using our proprietary Syno qPCR primer/probe design and synthesis platform. In under two weeks, we completed the synthesis of essential detection reagent materials, including amplification primers, probes, and quality controls.

Following the guidelines of the WHO, as well as the CDC in both China and the US, Synbio Technologies has developed a comprehensive series of primers and probe materials for COVID-19 detection, available in bulk. We offer a free trial package and ensure the supply of one million copies of nucleic acid diagnostic probes daily, speeding up disease detection and research.

Service Details

• SARS-CoV-2 Real Time RT-PCR Detection Assay

Box #1: Primers and Probes

Reagent Label	Part #	Description	Quantity /Tube	Reactions /Tube	Price
SARS-CoV-2_N1	ST202001	SARS-CoV-2_N1 Combined Primer/Probe Mix	37.5 µL/0.56 nmol	25	$229/Kit *
SARS-CoV-2_N2	ST202002	SARS-CoV-2_N2 Combined Primer/Probe Mix	37.5 µL/0.56 nmol	25
SARS-CoV-2_N3	ST202003	SARS-CoV-2_N3 Combined Primer/Probe Mix	37.5 µL/0.56 nmol	25
RP	ST202004	Human RNase P Forward Primer/Probe Mix	37.5 µL/0.56 nmol	25
2X Reaction Buffer	ST202006	Buffer, dNTP	1.2ml	100
Enzymes Mix	ST202007	UDG, Taq DNA Polymerase, Reverse Transcriptase, RNase inhibitor	200µL	100
Nuclease-free Water	ST202008	Nuclease-free Water	1.5ml	100

* Each part above can be sold separately, please contact us at service@synbio-tech.com.

Box #2: Positive Control

Reagent Label	Part #	Descriptions	Quantity	Note	Price
SARS-CoV-2 Positive Control (Plasmid DNA)	ST202005	The assays target regions within the SARS-CoV-2 nucleocapsid gene which is present in the SARS-CoV-2_N Positive Control plasmid. The Hs_RPP30 Control contains a portion of the RPP30 gene, a gene present in the human genome.	125 µL/1 tube	Providing (25) 5 µL test reactions	Price included *

* Positive Control Plasmid DNA included in Detection Assay Kit, $50 if sold separately.

Statements

This assay is for Research Use Only (RUO) and has not been tested on clinical samples.

This assay is designed based on CDC protocol and public SARS-CoV-2 genome sequences.

Different sample extraction methods, sample quality, operation parameters, data processing and other un-tested factors might impact this assay’s performance.

Stability tests and data are not available now due to the pandemic emergency. More information will be updated when those are ready.

Certificate of Analysis and Protocol

SARS-CoV-2 Realtime RT-PCR Detection Assay COA

SARS-CoV-2 Realtime RT-PCR Detection Assay Protocol

• Primer/Probe （WHO）

Catalog# SYN20200100, this set includes primers/probes of RdRp, E and N genes.

Name	Part #	Description	Oligonucleotide Sequence (5’>3’)	Length	Fluorophores/Quencher	Price
RdRP gene	ST20200101	Primer RdRP_SARSr-F2	GTGARATGGTCATGTGTGGCGG	22	100rxn/part, $30/set; 500rxn/part, $60/set
	ST20200102	Primer RdRP_SARSr-R1	CARATGTTAAASACACTATTAGCATA	26
	ST20200103	Probe RdRP_SARSr-P2	CAGGTGGAACCTCATCAGGAGATGC	25		FAM/BHQ1
	ST20200104	Probe RdRP_SARSr-P1	CCAGGTGGWACRTCATCMGGTGATGC	26		FAM/BHQ1
E gene	ST20200105	Primer E_Sarbeco_F1	ACAGGTACGTTAATAGTTAATAGCGT	26
	ST20200106	Primer E_Sarbeco_R2	ATATTGCAGCAGTACGCACACA	22
	ST20200107	Probe E_Sarbeco_P1	ACACTAGCCATCCTTACTGCGCTTCG	26		FAM/BHQ1
N gene	ST20200108	N_Sarbeco_F1	CACATTGGCACCCGCAATC	19
	ST20200109	N_Sarbeco_R1	GAGGAACGAGAAGAGGCTTG	20
	ST20200110	N_Sarbeco_P1	ACTTCCTCAAGGAACAACATTGCCA	25		FAM/BHQ1

* Each part above can be sold separately, please contact us at service@synbio-tech.com.

* Data Sheet and Instruction for Use

• Primer/Probe（China CDC）

Catalog# SYN20200200, this set includes primers/probes of Target 1(ORF1ab) and Target2(N).

Name	Part #	Description	Oligonucleotide Sequence (5’>3’)	Length	Fluorophores/Quencher	Price
Target 1 (ORF1ab)	ST20200201	Forward Primer 1ab	CCCTGTGGGTTTTACACTTAA	21		100rxn/part, $20/set; 500rxn/part, $40/set
	ST20200202	Reverse Primer 1ab	ACGATTGTGCATCAGCTGA	19
	ST20200203	Probe 1ab	CCGTCTGCGGTATGTGGAAAGGTTATGG	28	FAM/BHQ1
Target 2(N)	ST20200204	Forward Primer N	GGGGAACTTCTCCTGCTAGAAT	22
	ST20200205	Reverse Primer N	CAGACATTTTGCTCTCAAGCTG	22
	ST20200206	Probe N	TTGCTGCTGCTTGACAGATT	20	FAM/BHQ1

* Each part above can be sold separately, please contact us at service@synbio-tech.com.

• Primer/Probe（US CDC）

Catalog# SYN20200300, this set includes primers/probes of RP gene and 2019-nCOV N1/2/3 gene.

Name	Part #	Description	Oligonucleotide Sequence (5’>3’)	Length	Fluorophores/Quencher	Price
RP-F	ST20200301	RNase P Forward Primer	AGATTTGGACCTGCGAGCG	19		100rxn/part, $40/set; 500rxn/part, $80/set
RP-R	ST20200302	RNase P Reverse Primer	GAGCGGCTGTCTCCACAAGT	20
RP-P	ST20200303	RNase P Probe	TTCTGACCTGAAGGCTCTGCGCG	23	FAM/BHQ1
2019-nCoV_N1-F	ST20200304	2019-nCoV_N1 Forward Primer	GACCCCAAAATCAGCGAAAT	20
2019-nCoV_N1-R	ST20200305	2019-nCoV_N1 Reverse Primer	TCTGGTTACTGCCAGTTGAATCTG	24
2019-nCoV_N1-P	ST20200306	2019-nCoV_N1 Probe	ACCCCGCATTACGTTTGGTGGACC	24	FAM/BHQ1
2019-nCoV_N2-F	ST20200307	2019-nCoV_N2 Forward Primer	TTACAAACATTGGCCGCAAA	20
2019-nCoV_N2-R	ST20200308	2019-nCoV_N2 Reverse Primer	GCGCGACATTCCGAAGAA	18
2019-nCoV_N2-P	ST20200309	2019-nCoV_N2 Probe	ACAATTTGCCCCCAGCGCTTCAG	23	FAM/BHQ1
2019-nCoV_N3-F	ST20200310	2019-nCoV_N3 Forward Primer	GGGAGCCTTGAATACACCAAAA	22
2019-nCoV_N3-R	ST20200311	2019-nCoV_N3 Reverse Primer	TGTAGCACGATTGCAGCATTG	21
2019-nCoV_N3-P	ST20200312	2019-nCoV_N3 Probe	AYCACATTGGCACCCGCAATCCTG	24	FAM/BHQ1

* Each part above can be sold separately, please contact us at service@synbio-tech.com.

* Data Sheet and Instruction for Use

• Standards or References

Synbio Technologies has obtained the following viral RNA through in vitro transcription. We have prepared positive controls, helpful in controlling the reverse transcription efficiency, achieving the unification of standards, reducing the false negative rate, making the detection results more reliable, and greatly improving the accuracy of the kit detection degree.

Name	Catalog	Description	Transcription Sequence	Price (50 rxn)
RdRP gene	SYN20200401	RdRP standards	GTGAAATGGTCATGTGTGGCGGTTCAC TATATGTTAAACCAGGTGGAACCTCATCAGGA GATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTG	$50
E gene	SYN20200402	E gene standards	ACAGGTACGTTAATAGTTAATAGCG TACTTCTTTTTCTTGCTTTCGTGGTA TTCTTGCTAGTTACACTAGCCATCCTT ACTGCGCTTCGATTGTGTGCGTACTGCTGCAATAT	$50
Target 1 (ORF1ab)	SYN20200403	ORF1ab standards	CCCTGTGGGTTTTACACTTAAAAACACAGT CTGTACCGTCTGCGGTATGTGGAAAGGTTAT GGCTGTAGTTGTGATCAACTCCGCG AACCCATGCTTCAGTCAGCTGATGCACAATCGT	$50
Target 2 (N)	SYN20200404	N standards	GGGGAACTTCTCCTGCTAGAATGGCTGGC AATGGCGGTGATGCTGCTCTTGCTTTGCTGC TGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTG	$50
Endogenous control	SYN20200405	RNase P	AGATTTGGACCTGCGAGCGGGTTCT GACCTGAAGGCTCTGCGCGGACTTG TGGAGACAGCCGCTC	$50

* Each part above can be sold separately, please contact us at service@synbio-tech.com.

* Data Sheet and Instruction for Use

* The above figure shows the sample map of COVID-19 gene RNA extracted by Synbio Technologies (N-gene part, ORF1ab, E-gen part, RdRP gene part).

QUOTE

Therapeutic Strategy

Our exclusive CI system can facilitate accurate, fast, and high-quality gene synthesis of any sequence. Syno Ab is our de novo antibody design and production platform based on the combination of the DNA manufacturing with antibody molecule computer simulation design. Syno Ab helps researchers effectively reduce costs and time on designated target design, neutralizing antibody generation, and drug discovery.

Preventive Strategy

Syno Ab is our one-stop de novo antibody design and production platform based on the combination of antibody molecule computer simulation design technology with DNA manufacturing technology. It was developed for antibody drug discovery and can help researchers effectively reduce research costs and time. With a highly experienced bioinformatics team, Synbio Technologies provides guaranteed design for designated targets, neutralizing antibodies, and vaccine preparation. We can efficiently serve the construction of humanized antibody libraries, genetic engineering vaccine development, chromosome/genome synthesis, and more to meet various customer needs.

Catalog	Antibody	Antigen	Size	Price ($)	Availability
SYN-C1001-1	Nucleocapsid Antibody, Rabbit Pab,Antigen Affinity Purified	Nucleocapsid	50 μg	400	1 Week
SYN-C1001-2	Nucleocapsid Antibody, Rabbit Pab,Antigen Affinity Purified	Nucleocapsid	100 μg	650	1 Week
SYN-C1002-1	Nucleocapsid Antibody, Rabbit Mab	Nucleocapsid	50 μg	400	1 Week
SYN-C1002-2	Nucleocapsid Antibody, Rabbit Mab	Nucleocapsid	100 μg	650	1 Week
SYN-C1003-1	Nucleocapsid Antibody, Rabbit Mab	Nucleocapsid	50 μg	400	1 Week
SYN-C1003-2	Nucleocapsid Antibody, Rabbit Mab	Nucleocapsid	100 μg	650	1 Week
SYN-C1004-1	Spike S1 Antibody, Rabbit Mab	Spike S1	50 μg	400	1 Week
SYN-C1004-2	Spike S1 Antibody, Rabbit Mab	Spike S1	100 μg	650	1 Week
SYN-C1005-1	Spike Antibody,Chimeric Mab	Spike	50 μg	400	1 Week
SYN-C1005-2	Spike Antibody,Chimeric Mab	Spike	100 μg	650	1 Week
SYN-C1006-1	Spike RBD Antibody, Rabbit PAb, Antigen Affinity Purified	Spike RBD	50 μg	400	1 Week
SYN-C1006-2	Spike RBD Antibody, Rabbit PAb, Antigen Affinity Purified	Spike RBD	100 μg	650	1 Week
SYN-C1007-1	Spike S2 Antibody, Rabbit PAb, Antigen Affinity Purified	Spike S2	50 μg	400	1 Week
SYN-C1007-2	Spike S2 Antibody, Rabbit PAb, Antigen Affinity Purified	Spike S2	100 μg	650	1 Week