Adeno-associated Virus Packaging

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Adeno-associated viruses (AAVs) are small, non-enveloped viruses with an icosahedral capsid and a single-stranded DNA genome of approximately 4.7 kb. Renowned for their broad host range, low immunogenicity, high safety profile, and stable gene expression, AAVs are ideal for gene therapy applications.

AAVs are available in various serotypes, each with unique cellular receptor affinities that influence tissue-specific targeting and transfection efficiency. Synbio Technologies offers a range of AAV serotypes for packaging services, with vectors specifically designed to ensure long-term gene expression in actively dividing cells with minimal toxicity, making them suitable for a wide range of animal research studies.

Serotypes	Tissues
AAV1	CNS, heart, skeletal muscle, smooth muscle, retina, endothelial cell
AAV2	CNS, retina, liver, brain, kidney, smooth muscle
AAV5	CNS, smooth muscle, retina, lung, brain
AAV6	Lung, heart
AAV-6m	Liver, muscle
AAV7	CNS, retina, liver, muscle
AAV-7m8	Retina
AAV8	CNS, liver, adipose, retina, muscle, pancreas
AAV-Pan	Pancreas（intraperitoneal injection）
AAV-Anc80L65	Inner ear, retina
AAV9	CNS, heart, lung, liver, retina, skin, skeletal muscle
AAV-Retro	CNS：retrograde tracers
AAV11	CNS：improved distribution characteristics and retrograde transportation efficiency compared to AAV/Retro
AAV13	Precise in situ labeling in brain parenchyma
AAV-Cmg	Microglia with high efficiency, in-vitro
AAV-MG1.2	Microglia with high efficiency, in-vivo
AAV-CPP.16	Blood-brain barrier in rodents and non-human primates
AAV-DJ	Retina, liver, kidney, in-vitro
AAV-DJ-8	Liver, retina, CNS, muscle
AAV-DJ-9	Retrograde tracer nonsynaptic labeling in songbird neural circuits
AAV-Mac	CNS in non-human primates, across the blood-brain barrier (intravenous injection)
AAV-MacPNS1	PNS in rodent, PNS and CNS in macaque and marmoset (systemic administration)
AAV-MacPNS2	PNS in rodent, PNS and CNS in macaque and marmoset (systemic administration)
AAV-olig001	Oligodendrocytes
AAV-PHP. eB	CNS: crossed the blood-brain barrier, placental barrier
AAV-PHP. S	PNS: crossed the blood-brain barrier, placental barrier
AAV-B10	CNS: crossed the blood-brain barrier, not enriched in the liver
AAV-B22	CNS: crossed the blood-brain barrier, not enriched in the liver
AAV-Bl30	Brain microvasculature endothelial cell
AAV-BR1	Brain microvasculature endothelial cell
AAV-rh10	CNS, liver, heart, lung, muscle, in-vitro
AAV-Ark313	Mammalian T cell

Tissues	Promoter	Characteristics	Tissues	Promoter	Characteristics
Neurons	hSyn	Neuron-specific promoter	Blood Vessel	EnSM22a	Vascular smooth muscle-specific promoter
	mecp2	Shorter neuron-specific promoter		ICAM2	Vascular endothelial-specific promoter
	c-fos	Excitatory neuron promoter		CD68	Activated microglia-specific promoter
	CaMKⅡa	Forebrain excitatory neurons		F4/80	Macrophage-specific promoter
	hVGAT	GABAergic neuron/interneuron-specific promoter	Liver	TBG	Liver-specific promoter
	gfaABC1D	Astrocyte-specific promoter		ALB	Liver-specific promoter
	Iba1	Microglia-specific promoter		ApoEHCR-hAAT	Liver-specific promoter
	NSE	Neuron-specific promoters	Pancreas	Ins2	Pancreatic beta-cell-specific promoter
	GFAP104	Astrocyte-specific promoter	Pancreas	PDX1	Pancreatic beta-cell-specific promoter
	ALDH1L1	L1 Hypothalamic astrocyte-specific promoter	Bone	COL2A1	Chondrocyte-specific promoter
	Somatostat (SST)	SST subtype-specific promoter of gamma aminobutyric acidergic inhibitory neurons	Bone	mRUNX2	Osteoblast early-specific promoter
	MBP	Oligodendrocyte-specific promoter	Retina	rpe65	Retina-specific promoter
	CNP	Oligodendrocyte or Schwann cell-specific promoter	Retina	VMD2	Retinal pigment epithelium-specific promoter
Muscle	cTNT	Cardiomyocyte-specific promoter	Kidney	NPHS1	Kidney podocyte-specific promoter
	Nkx2.5	Cardiomyocyte early-specific promoters	Lung	SPB	Lung epithelial cell-specific promoter
	MCK	Muscle-specific promoter	Lung	SPC	Lung epithelial cell-specific promoter
	MHCK7	Muscle-specific promoter	Skin	K14	Keratin promoter
	MYOG	Cardiac fibroblast-specific promoter	Adipose Tissue	FABP4	Adipocyte-specific promoter
			Endothelium	TIE1	Endothelial cell-specific promoters

Highlights

Low Immunogenicity and Non-Pathogenic
Long-term Stable Expression
Diverse Serotypes and Broad Host Range
Tailored Experimental Protocols

Service Details

Adeno-associated Virus Packaging
Service Types	Titer	Volume	Turnaround Time
RNAi Adeno-associated Virus	110¹²vg/ml or 110¹³vg/ml	1ml	20-25 Business Days
sgRNA Adeno-associated Virus	110¹²vg/ml or 110¹³vg/ml
Overexpression Adeno-associated Virus	110¹²vg/ml or 110¹³vg/ml

QUOTE

Deliverables

Free control virus

Virus liquid, ≥10¹² vg/ml or ≥10¹³ vg/ml or, 200 μL/tube

QC report

FAQs

What are ITRs, and why are they important?

Inverted terminal repeats (ITRs) are sequences located at both ends of the AAV genome. They are crucial for the replication and packaging of the AAV vector, aiding in the formation of double-stranded DNA and ensuring the stability and integration of the vector into the host cell.

How is AAV vector purification performed?

AAV vector purification typically involves several steps:

· Cell lysis: Breaking open cells to release AAV particles.

· Clarification: Removing cell debris.

· Chromatography: Using methods such as affinity chromatography or ion-exchange chromatography to purify AAV particles.

· Ultracentrifugation: Concentrating and further purifying the AAV vector using gradient centrifugation.

What are some applications of AAV vectors?

· Gene therapy: Used for treating genetic disorders by delivering therapeutic genes.

· Gene editing: Serve as delivery vehicles for CRISPR/Cas9 and other gene-editing tools.

· Basic research: Used for studying gene function and cellular processes.

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