The General Introduction of Error-prone scFv Variant Antibody Libraries
Synthetic antibody libraries are instrumental in designing, constructing, and successfully generating highly functional antibodies for various applications. Their optimized framework sequences provide a strong scaffold, while controlled diversity in the CDRs offers distinct advantages over natural antibodies.
Random mutagenesis and error-prone PCR are commonly used to create diverse scFv
antibody libraries. The mutagenesis can target the entire V(D)J region or only the complementarity-determining regions (CDRs) of the antibody. As a result, mutations can occur throughout the entire scFv sequence or be concentrated in the CDRs., These libraries can be extremely large, offering a wide range of options for in vitro screening and expanding possibilities in antibody functionality research.
Highlights
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- High Diversity
Quality design leads to high diversity of the recombinant antibody libraries
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- High Quality Libraries
The updated phage display system provides a guarantee for larger storage capacity and highly functional antibodies
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- Cost-Effective
Competitive prices and affordable services
Service Details
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Process
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Details
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Deliverables
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Turnaround Time
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Template sequence construction
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Clone the template sequence in pUC57 vector.
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• Template sequence construct
• Variable fragment phagemid library and COA
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1-2 weeks
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Vector construction
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Perform error-prone PCR and clone scFv in the antibody expression vectors.
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2-3 weeks
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Transform E.coli and Library constructio
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Transform the constructs into TG1 competent cells, then prepare the plasmid and identify the capacity and accuracy of the library.
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2-3 weeks
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Workflow
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