Synbio Technologies’ high throughput synthesis platform utilizes inkjet technology to synthesize millions of oligonucleotide sequences on a chip in a single parallel run (up to 300 nt.) During the synthesis process, there is zero cross-contamination and the synthesized sequences are highly accurate and homogeneous. The customized synthesis service will perfectly match customers' downstream experiments and applications.

Applications

With synthesized oligo pools, our customers can then streamline various downstream applications within their research. These downstream applications include:

● Generating native DNA sequences

● Creating de novo DNA sequences

● Building genes, chassis, operons, pathways, and genomes

● Building DNA variant libraries

● Improving the features of a protein

● Testing all orthologs of a gene

● Optimizing any antibody through affinity maturation

Synbio Technologies can insert up to 20 Trimer sites in a single Trimer oligo to ensure the diversity and convenience of downstream screening. We provide a variety of amino acid customization services according to the actual needs of customers. Trimer oligo synthesis can be used for downstream library construction, used widely in protein directed evolution, antibody screening, drug target screening/drug discovery, enzyme optimization. and other research.

Case Study

Synbio Technologies delivers highly customized trimer libraries with amino acid types and proportions that meet customer requirements.

Take the sequence below as an example, the X represents the codon position where the mutation is required.

ACCG*************TGCCX1X2X3X4X5X6TGTX7X8X9X10X11X12X13X14X15X16X17TACX18TACT*************ACTT

X1 Trimer=A(80%):G(3%):R(3%):S(3%):T(8%):V(3%)

Table 1 The ratio of the synthesized amino acids with the actual requirements (Only partial amino acids are shown)

Table 2. The actual standard deviation of trimer oligos provided by Synbio Technologies

*The deviation between the actual proportion of trimer oligos delivered by Synbio Technologies and the proportion required by customers is small.  This proves that the quality of trimer oligos delivered is high.

Synbio Technologies provides one-stop gene function screening services based on the CRISPR-Cas9 sgRNA library system. With our proprietary Syno 3.0 DNA synthesis platform, we can design and synthesize high quality sgRNA library oligos in a fast and efficient manner to meet the needs of customers for sgRNA library construction.