Vector Libraries _Synbio Technologies

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Vector Libraries

Synbio Technologies' vector libraries offer you a one-stop service withextensive vector selection. The libraries contain vectors for various species such as E. coli, yeast, and mammals, catering to your downstream research needs. Existing vectors are readily available for immediate use! Need to modify the vectors? Just edit and synthesize!

We provide an online editing function to make vector modification more efficient, allowing you to edit and customize your desired vectors in real-time. Our efficient synthesis service ensures rapid and accurate delivery of vectors. Synbio Technologies' vector construction platform is a powerful and reliable ally for your scientific research projects!

Service Details

Cloning Vectors

• Cloning VectorsView More

Cloning Vectors
Vector Details	Customization	Standard
Species	Online Editing	Name	Recommend Index	Order
The target gene is inserted into the MCS (Multiple Cloning Site) region. The desired antibiotic gene sequence is inserted into the resistance marker region of E.coli. These operations are primarily used for the enrichment of the target gene sequence.	E.coli		MCS-Amp, Normal Gene Synthesis	*****	Inquiry
MCS-Kan, Normal Gene Synthesis
	A181-Amp, Normal Gene Synthesis
A181-Kan, Normal Gene Synthesis
	pCA, Complex Gene Synthesis
pCK, Complex Gene Synthesis

Expression Vectors

• Normal Gene Expression VectorsView More

Normal Gene Expression Vectors
Vector Details	Customization		Standard
Vector Details	Species	Online Editing	Name	Virus Packaging	Recommend Index	Order
Promoter1 initiates the expression of the target gene fused to the C-terminus of the Marker gene (inserted in the MCS region) Promoter2 initiates the expression of the resistance gene. It can be transiently or stably expressed in the cells 。	Mammal		pCMV:EGFP/MCS-pSV40:NeoR	/	*****	Inquiry

Normal Gene Expression Vectors
Vector Details	Customization		Standard
Vector Details	Species	Online Editing	Name	Virus Packaging	Recommend Index	Order
Promoter1 initiates the expression of the target gene fused to the N-terminus of the Marker gene (inserted in the MCS region). Promoter2 initiates the expression of resistance genes. It can be transiently or stably expressed in the cells.	Mammal		pCMV:MCS-EGFP-pSV40:NeoR	/	*****	Inquiry

Normal Gene Expression Vectors
Vector Details	Customization		Standard
Vector Details	Species	Online Editing	Name	Virus Packaging	Recommend Index	Order
Promoter1 is a mammalian cell expression promoter, which initiates the expression of target genes in cells. t7 promoter can initiate the expression of the target gene in specific E.coli, and can also achieve the transcription of the target gene in vitro. Promoter2 initiates the expression of the resistance genes. It can be transiently or stably expressed in the cells.	Mammal		pCMV-pT7:EGFP-pSV40:NeoR	/	*****	Inquiry

Normal Gene Expression Vectors
Vector Details	Customization		Standard
Vector Details	Species	Online Editing	Name	Virus Packaging	Recommend Index	Order
Promoter simultaneously initiates the expression of the target gene (MCS insertion) and the resistance gene; the target gene and the resistance gene are connected by the ORF linker. It can be transiently or stably expressed by lentivirus infection.	Mammal		pCMV:MCS-IRES-Puro	entivirus	****	Inquiry
	Mammal		pEF1a:MCS-IRES-Puro	entivirus	****	Inquiry

Normal Gene Expression Vectors
Vector Details	Customization		Standard
Vector Details	Species	Online Editing	Name	Virus Packaging	Recommend Index	Order
A promoter initiates target gene expression (MCS insertion). There is no eukaryotic cell screening marker. The vector is small, and the expression level is high. Only transient expressions can be performed.	Mammal		pCAGGS:MCS	/	***	Inquiry

• Recombinant Protein Expression VectorsView More

Recombinant Protein Expression Vectors
Vector Details	Customization		Standard
Vector Details	Expression System	Online Editing	Name	Recommend Index	Order
Constitutive expressions under the T7 promoter, the N-terminus and C-terminus of the target protein can be added with 0-1 purified tags, respectively. E.coli resistance screening is optional.	E.coli		pT7:T7 Tag/MCS/6×His；Amp	*****	Inquiry
	E.coli		pT7:T7 Tag/MCS；Amp	*****	Inquiry

Recombinant Protein Expression Vectors
Vector Details	Customization		Standard
Vector Details	Expression System	Online Editing	Name	Recommend Index	Order
IPTG induced the expression under the T7 promoter, and a purified Tag1 could be added to the N-terminus of the target protein (inserted in the MCS region). A protein cleavage site can be inserted between Tag1 and the target protein to remove the tag of the target protein. E.coli resistance screening is optional.	E.coli		pT7lac:His-Thrombin/MCS；Amp	*****	Inquiry
			pT7lac:His-Factor Xa/MCS；Amp	*****
			pT7lac:His-Enterokinas/MCS；Amp	*****

Recombinant Protein Expression Vectors
Vector Details	Customization		Standard
Vector Details	Expression System	Online Editing	Name	Recommend Index	Order
IPTG induced the expression under the T7 promoter, and a signal peptide could be added to the N-terminus of the target protein (inserted in the MCS region) for protein localization. A Tag1 can be added to the C-terminal of the target. E.coli resistance screening is optional.	E.coli		pT7lac:pelB/MCS/His Tag；Amp	*****	Inquiry
	E.coli		pT8lac:pelB/MCS/His Tag；KanR	*****	Inquiry

Recombinant Protein Expression Vectors
Vector Details	Customization		Standard
Vector Details	Expression System	Online Editing	Name	Recommend Index	Order
IPTG induced the expression under the T7 promoter, and the N-terminal and C-terminal of the target protein could be added with 0-1 purification tags, respectively. E.coli resistance screening is optional.	E.coli		pT7lac:MCS/His Tag；Amp	*****	Inquiry
			pT7lac:T7 Tag/MCS/His Tag；Amp	*****
			pT7lac:T7 Tag/MCS/His Tag；KanR	*****

Recombinant Protein Expression Vectors
Vector Details	Customization		Standard
Vector Details	Expression System	Online Editing	Name	Recommend Index	Order
IPTG induced the expression under the T7 promoter. Two purification tags can be added to the N-terminus of the target protein. A protein cleavage site can be added between the two tags for tag removal. A purification tag can be added at the C-terminus. E.coli resistance screening is optional.	E.coli		pT7lac:6×His-Thrombin site-T7 tag/MCS/6×His;KanR	*****	Inquiry
			pT7lac:S Tag-Thrombin site/MCS/6×His;KanR	*****
			pT7lac:6×His-Enterokinase site/MCS/S Tag；Amp	*****

Recombinant Protein Expression Vectors
Vector Details	Customization		Standard
Vector Details	Expression System	Online Editing	Name	Recommend Index	Order
IPTG induced the expression under the T7 promoter. Two purification tags can be added to the N-terminus of the target protein. A protein cleavage site can be added between the Tag1 and Tag2 tags for tag removal. A protein cleavage site can be added between Tag2 and the target protein. A purification tag can be added at the C-terminus. E.coli resistance screening Resistance is optional.	E.coli		pT7lac:6×His-Thrombin site-S tag-Enterokinase site/MCS/6×His；KanR	*****	Inquiry

Recombinant Protein Expression Vectors
Vector Details	Customization		Standard
Vector Details	Expression System	Online Editing	Name	Recommend Index	Order
IPTG induced the expression under the T7 promoter. Three purification tags can be added to the N-terminus of the target protein. A protein cleavage site can be added between the Tag2 and Tag3 tags. A protein cleavage site can be added between Tag3 and the target protein, and a purification tag can be added at the C-terminus. E.coli resistance screening is optional.	E.coli		pT7lac:TrxA-6×His-Thrombin site-S Tag-Enterokinase site-6×His；Amp	*****	Inquiry

Recombinant Protein Expression Vectors
Vector Details	Customization		Standard
Vector Details	Expression System	Online Editing	Name	Recommend Index	Order
Cold treatment induced expression under the cspA promoter. A purification tag can be added to the N-terminus of the target protein. E.coli resistance screening is optional.	E.coli		pcspAlac：cspA5’UTR-6×His/MCS/csp3’UTR；Amp	*****	Inquiry

Recombinant Protein Expression Vectors
Vector Details	Customization		Standard
Vector Details	Expression System	Online Editing	Name	Recommend Index	Order
IPTG inducedthe expression under the tac promoter, and one tag could be added to the N-terminus of the target protein (MCS). A protein cleavage site can be added between the Tag and the target protein.	E.coli		ptac:GST-HRV 3C site-MCS;Amp	*****	Inquiry
	E.coli		ptac:GST-Thrombin site-MCS;Amp	*****	Inquiry

Recombinant Protein Expression Vectors
Vector Details	Customization		Standard
Vector Details	Expression System	Online Editing	Name	Recommend Index	Order
IPTG induced the expression of the two T7 promoters. A Tag can be added to the first expression cassette at the N-terminus of the target protein (MCS1), and a protein cleavage site can be added between the target protein and MCS1. The second expression cassette can add a Tag at the C-terminus of the target protein. Ori is optional, screening resistance and promoter is optional.	E.coli		pT7lac:8×His-TEV site/MCS-pT7lac:MCS/S Tag; pcat:CmR；p15Aori	*****	Inquiry
			pT7lac:6×His/MCS-pT7lac:MCS/S Tag；pcat:CmR；p15Aori	*****
			pT7lac:MCS-pT7lac:MCS/S Tag; pAmp:KanR;RSF ori	*****
			pT7lac:MCS-pT7lac:MCS/S Tag; pAmp:SmR;CloDF13 ori	*****

Recombinant Protein Expression Vectors
Vector Details	Customization		Standard
Vector Details	Expression System	Online Editing	Name	Recommend Index	Order
Methanol induced the expression under the AOX1 promoter, and the screening marker is optional.	Pichia pastoris		pAOX1:a-factor secretion signal/MCS;Amp/HIS4（KM71、GS115）	***	Inquiry

Recombinant Protein Expression Vectors
Vector Details	Customization		Standard
Vector Details	Expression System	Online Editing	Name	Recommend Index	Order
Methanol induced expression under the AOX1 promoter. A signal peptide can be added before the target protein (MCS1) to mediate the expression and localization of the target protein. Two Tags can be added at the C-terminus, and there is a MCS region between Tag1 and Tag2, which can be selectively added. Screening markers can be selected to induce stable expression.	Pichia pastoris		pAOX1:a-factor secretion signal/MCS/Myc-MCS-6×His；Zeocin	***	Inquiry

Recombinant Protein Expression Vectors
Vector Details	Customization		Standard
Vector Details	Expression System	Online Editing	Name	Recommend Index	Order
Expression of the target gene under the promoter. A purification tag can be added to the N-terminus and C-terminus of the target protein, and a protein cleavage site can be added between the N-terminus purification tag and the plaque protein for tag removal. This is a stable expression.	Pichia pastoris		pAOX1:a-factor secretion signal/MCS/Myc-MCS-6×His；Zeocin	***	Inquiry

Recombinant Protein Expression Vectors
Vector Details	Customization		Standard
Vector Details	Expression System	Online Editing	Name	Recommend Index	Order
Methanol induced expression under the AOX1 promoter. A signal peptide can be added before the target protein (MCS1) to mediate the expression and localization of the target protein. Two Tags can be added at the C-terminus, and there is a MCS region between Tag1 and Tag2, which can be selectively added. Screening markers can be selected to induce stable expression.	Pichia pastoris		pAOX1:a-factor secretion signal/MCS/Myc-MCS-6×His；Zeocin	***	Inquiry

Recombinant Protein Expression Vectors
Vector Details	Customization		Standard
Vector Details	Expression System	Online Editing	Name	Recommend Index	Order
Expression of the target gene under the promoter. A purification tag can be added to the N-terminus and C-terminus of the target protein, and a protein cleavage site can be added between the N-terminus purification tag and the plaque protein for tag removal. This is a stable expression.	Insect Cell		pPH:6×His-TEV site-MCS/Flag,Amp/GmR	*****	Inquiry
			pPH:6×His-TEV site-MCS,Amp/GmR	*****
			pPH:MCS,Amp/GmR	*****

Recombinant Protein Expression Vectors
Vector Details	Customization		Standard
Vector Details	Expression System	Online Editing	Name	Recommend Index	Order
The expression of the two genes was initiated by two promoters. This is a stable expression。	Insect Cell		pPH:MCS-pP10:MCS，Amp/GmR	***	Inquiry

• Non-coding RNA Expression VectorsView More

Non-coding RNA Expression Vectors
Vector Details	Customization	Standard
Expression System	Online Editing	Name	Recommend Index	Order
The target promoter initiates the expression of the target shRNA. It can be expressed instantaneously or stably, which can replace lentivirus packaging	Mammal		pU6:MCS-CPPT/CTS，Amp/Puro	*****	Inquiry

Editing Vectors

• DNA Editing VectorsView More

DNA Editing Vectors
Vector Details	Customization		Standard
Vector Details	Species	Online Editing	Name	Recommend Index	Order
The sgRNA and spCas9 were expressed on the same plasmid vector. There are two promoters respectively. Screening Marker is optional, while spCas9 can be optimized according to Species, which belongs to CRISPR spCas9 knockout vector and can be packaged by lentivirus.	Mammal		pU6:sgRNA-pEF1a:Cas9-P2A-Puro；Amp	*****	Inquiry
			pU6:sgRNA-pEF1a:Cas9-P2A-Blast；Amp
			pU6:sgRNA-pEF1a:Cas9-P2A-GFP；Amp
			pU6:sgRNA-pEF1a:Cas9-P2A-mCherry-T2A-Puro；Amp

DNA Editing Vectors
Vector Details	Customization		Standard
Vector Details	Species	Online Editing	Name	Recommend Index	Order
The sgRNA and spCas9 were expressed on the same plasmid vector, with two promoters respectively. Selection Marker is optional. spCas9 can be optimized according to Species and belongs to the CRISPR spCas9 knockout vector. This is a non-viral packaging vector. It can be transiently or stably expressed.	Mammal		pU6:sgRNA-pCBh: Cas9-T2A-Puro；Amp	*****	Inquiry
	Mammal		pU6:sgRNA-pEF1a: Cas9-P2A-mCherry-T2A-Puro；Amp	****	Inquiry

DNA Editing Vectors
Vector Details	Customization		Standard
Vector Details	Species	Online Editing	Name	Recommend Index	Order
The sgRNA and saCas9 were expressed on the same plasmid vector, and there were two promoters respectively. Selection Marker is optional. CRISPR Cas9 can be optimized according to Species and belongs to the CRISPR SaCas9 knockout vector This is an adenovirus packaging vector. It can be transiently or stably expressed.	Mammal		pCMV:SaCas9-T2A-mCherry-pU6: sgRNA；Amp	****	Inquiry
	Mammal		pCMV:SaCas9-pU6:sgRNA；Amp	****	Inquiry

QUOTE

Intellectual Property Rights StatementView More

Synbio Technologies designs and manufactures nucleic acid vectors based on customer’s requirements. While we participate in the design and manufacturing of these vectors, it does not imply that we possess any intellectual property rights over them or the right to transfer such intellectual property rights, unless otherwise agreed upon by both parties.

The customer understands and agrees that the use of the vectors designed and ordered by the customer, as well as the related vector components, is solely at the discretion and responsibility of the customer. When purchasing products and services from Synbio Technologies, the customer should be fully aware that they may need to obtain licenses or authorizations from relevant third-party intellectual property owners. Any disputes or claims arising from the customer's purchase of products or use of services that may be related to third-party intellectual property rights shall be solely the responsibility of the customer, and Synbio Technologies shall not bear any responsibility for them.

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