Standard Human CRISPR Libraries
Standard Mouse CRISPR Libraries
Standard Poultry CRISPR Libraries
Standard Animal CRISPR Libraries
Cloning Vectors
Vector Number | Functional Description | Name |
Prokaryotic Screening Resistance / Marker |
Eukaryotic Screening Resistance / Marker |
Order |
---|---|---|---|---|---|
A01 | Conventional Gene Synthesis Vector | MCS | Amp | N/A | Inquiry |
A02 | Conventional Gene Synthesis Vector | MCS | Kan | N/A | Inquiry |
A03 | Conventional Gene Synthesis + Prokaryotic-Induced Expression Vector | pTet:MCS | Amp | N/A | Inquiry |
A04 | Conventional Gene Synthesis + Prokaryotic-Induced Expression Vector | pLac:MCS | Amp | N/A | Inquiry |
A05 | Conventional Gene Synthesis Vector | MCS | CmR | N/A | Inquiry |
A06 | Conventional Gene Synthesis + Prokaryotic-Induced Expression Vector | pLac:MCS | Amp | N/A | Inquiry |
A07 | Conventional Gene Synthesis + Prokaryotic-Induced Expression + dsRNA IVT Vector | pT7:MCS:pS6 | Amp | N/A | Inquiry |
A08 | Conventional Gene Synthesis + Prokaryotic-Induced Expression Vector | pLac:MCS | Amp | N/A | Inquiry |
A09 | Conventional Gene Synthesis + Prokaryotic-Induced Expression Vector | pLac:MCS | Kan | N/A | Inquiry |
A10 | Complex Gene Synthesis Vector | pLac:MCS | Amp | N/A | Inquiry |
A11 | Complex Gene Synthesis Vector | pLac:MCS | Kan | N/A | Inquiry |
Expression Vectors
Vector Number | Species | Functional Description | Name |
Prokaryotic Screening Resistance / Marker |
Eukaryotic Screening Resistance / Marker |
Order |
---|---|---|---|---|---|---|
Bm01 | Mammal | The CMV promoter was initiated and the target gene was fused to the C-terminus of EGFP. It can be instantaneously transformed and stably transformed. Non-lentivirus packaging vector. The target cells were screened by green fluorescence and Neo. | pCMV:EGFP-MCS | Kan | Neo/EGFP | Inquiry |
Bm02 | The CMV promoter was initiated and the target gene was fused to the N-terminus of EGFP. It can be instantaneously transformed and stably transformed. Non-lentivirus packaging vector. The target cells were screened by green fluorescence and Neo. | pCMV:MCS-EGFP | Kan | Neo/EGFP | Inquiry | |
Bm03 | CMV initiates the expression of the target gene, which is located at the N-terminus of EYFP. It can be transiently expressed or stably expressed. Non-lentiviral packaging vector, expressed only in eukaryotic cells. Cells were screened by Neo and EYFP. | pCMV:MCS-EYFP | Kan | Neo/EYFP | Inquiry | |
Bm04 | CMV initiates the expression of the target gene, which is located at the N-terminus of ECFP. It can be transiently expressed or stably expressed, non-lentiviral packaging vector, expressed only in eukaryotic cells. Cells were screened by Neo and ECFP. | pCMV:MCS-ECFP | Kan | Neo/ECFP | Inquiry | |
Bm05 | The CMV promoter was initiated and the target gene was fused to the N-terminus of EGFP. It can be instantaneously transformed and stably transformed. Non-lentivirus packaging vector, target cells were screened by green fluorescence and Neo. | pCMV:MCS-EGFP | Kan | Neo/EGFP | Inquiry | |
Bm06 | The CMV promoter was initiated and the target gene was located at the N-terminus of EGFP. It can be instantaneously transformed or stably transformed. The non-lentiviral packaging vector can be expressed in both prokaryotic cells and eukaryotic cells. The target cells were screened by Kan / Neo. | pCMV-pT7:MCS-EGFP | Amp | Neo | Inquiry | |
Bm07 | CMV initiates the expression of the target gene, which is located at the C-terminus of mCherry. It can be transiently expressed or stably expressed. Non-lentivirus packaging vector, expressed only in eukaryotic cells. Cells were screened by Neo and mCherry. | pCMV:mCherry-MCS | Kan | Neo/mCherry | Inquiry | |
Bm08 | The CMV promoter was initiated, and the target gene was fused to the N-terminus of EGFP through IRES. It can be instantaneously transformed and stably transformed. Non-lentivirus packaging vector, target cells were screened by green fluorescence and Neo. | pCMV:MCS/IRES/EGFP | Kan | Neo/EGFP | Inquiry | |
Bm09 | The CMV promoter was initiated, and the target gene was fused to the N-terminus of ZsGreen1 through IRES. It can be instantaneously transformed and stably transformed. Non-lentivirus packaging vector, target cells were screened by green fluorescence and Neo. | pCMV:MCS/IRES/ZsGreen1 | Kan | Neo/ZsGreen1 | Inquiry | |
Bm10 | CMV initiates target gene expression. The target gene is fused to the N-terminus of DsRed by IRES. It can be transiently expressed and stably expressed. Non-lentivirus packaging vector, screening cells by Neo. | pCMV:MCS/IRES/DsRed | Kan | Neo/DsRed | Inquiry | |
Bm11 | CMV initiates expression, and the C-terminus and C-terminus of DsRed2 contain ER targeting sequences. Non-lentiviral packaging vectors can be used to label the endoplasmic reticulum of living cells. The target protein is located at the C-terminal of DsRed2. | pCMV:DsRed-MCS | Kan | Neo/DsRed2 | Inquiry | |
Bm12 | There are two EF1a / CMV promoters that can initiate the expression of two target genes, respectively. It can be transiently expressed or stably expressed. Non-lentiviral packaging vector, containing Puro and DHFR screening marker genes, can be screened with MTX and Puro at the same time. | pCMV/EF1a:MCS-pCMV/EF1a:MCS | Kan | Puro/DHFR | Inquiry | |
Bm13 | The target gene and copGFP were expressed by CMV and EF1a promoter, respectively. It can be instantaneously transformed and stably transformed. Lentivirus packaging, through green fluorescence detection, screening of target cells. | pCMV:MCS-pEF1:copGFP | Amp | copGFP | Inquiry | |
Bm14 | CMV promoter initiates target gene expression. It can be instantaneously transformed and stably transformed. Lentivirus packaging can be performed. The target cells were screened by Puro resistance. | pCMV:MCS-pEF1:Puro | Amp | Puro | Inquiry | |
Bm15 | The transcription of the target gene and the resistance gene Puro was initiated by the EF1a promoter. There is a T2A peptide between the target gene and the resistance gene, which can be self-cut at the translation level to ensure that the target gene and the resistance gene have the same translation level. The lentiviral packaging plasmid was pPACKH1. It can be transiently expressed or stably expressed. Lentiviral packaging vector, expressed only in eukaryotic cells. Cells were screened by Puro. | pEF1a:MCS/T2A/Puro | Amp | Puro | Inquiry | |
Bm16 | EF1a initiates target gene expression. The copGFP / T2A / Puro expression was initiated by the CMV promoter. It can be transiently transformed and stably expressed. Lentivirus packaging vector, screening cells by copGFP and Puro. | pEF1a:MCS-pCMV:copGFP-T2A-Puro | Amp | Puro/copGFP | Inquiry | |
Bm17 | It is initiated by the CMV promoter. The target gene was fused to the N-terminus of Hygro by IRES. It can be instantaneously transformed and stably transformed. Lentiviral packaging vector, target cells were screened by Hygro. | pCMV:MCS/IRES/Hygro | Amp | Hygro | Inquiry | |
Bm18 | CMV initiates the expression of the target gene, which is located at the C-terminus of mCherry. It can be transiently expressed or stably expressed. Lentiviral packaging vector, expressed only in eukaryotic cells. Cells were screened by Puro and mCherry. | pCMV:mCherry-MCS | Amp | Puro/mCherry | Inquiry | |
Bm19 | CMV initiates the expression of the target gene, which is fused to the N-terminus of AcGFP1 through IRES. It can be transiently expressed or stably expressed. Lentivirus packaging vector, screening cells by AcGFP1 and Puro. | pCMV:MCS/IRES/AcGFP1 | Amp | Puro/AcGFP1 | Inquiry | |
Bm20 | CMV initiates target gene expression. It can be instantaneously transformed or stably transformed. Lentiviral packaging vector, the target cells were screened by Puro. | pCMV:MCS | Amp | Puro | Inquiry | |
Bm21 | Tetracycline promoter (TRE3GS) regulates target gene expression (integrating regulation and response functions). It can be transiently expressed or stably expressed. Lentiviral packaging vector, expressed only in eukaryotic cells. Cells were screened by Puro. | pTRE3Gs:MCS | Amp | Puro | Inquiry | |
Bm22 | The expression of target gene and resistance gene Puro was initiated by CMV promoter. There is an IRES sequence between the target gene sequence and the resistance gene, which can completely separate the two protein sequences, but the expression ability of the latter is lower than that of the former. It can be transiently expressed or stably expressed. Lentiviral packaging vector, expressed only in eukaryotic cells. Cells were screened by Puro. | pCMV:MCS/IRES/Puro | Amp | Puro | Inquiry | |
Bm23 | The target gene expression was initiated by the EF1a promoter, and the C-terminus of the target gene was fused with GFP. Lentivirus packaging can be performed. | pEF1a:MCS/EGFP | Amp | GFP | Inquiry | |
Bm24 | Two CMV promoters were used to initiate the simultaneous expression of the target gene and GFP. Low copy expression vector can be used for adenovirus packaging. | pCMV:MCS-pCMV:GFP | Kan | EGFP | Inquiry | |
Bp01 | Plant | Two 35S promoters were used to initiate the expression of target gene and GUS gene, respectively. It can be transiently expressed, and can also be transfected into plant cells by Agrobacterium for stable expression. | p35S:MCS-p35S:GUS | Kan | Hygro | Inquiry |
Bp02 | The 35S promoter initiates the expression of the target gene. The C-terminal of the target gene was fused with EGFP protein. It can be transiently expressed, and can also be transfected into plant cells by Agrobacterium for stable expression. | p35S:MCS/EGFP | Kan | Hygro/EGFP | Inquiry | |
Bp03 | The UBi promoter initiates target gene expression. The 35S promoter initiates EGFP expression. It can be transiently expressed, and can also be transfected into plant cells by Agrobacterium for stable expression. | pUbi:MCS-p35S:EGFP | Kan | EGFP | Inquiry | |
Bp04 | The 35S promoter initiates the expression of mCherry fluorescence located in the Golgi apparatus. It can be transiently expressed, and can also be transfected into plant cells by Agrobacterium for stable expression. | p35S:mCherry/Golgi | Kan | Hygro/mCherry | Inquiry | |
Bp05 | The 35S promoter initiates ER / CFP gene expression. CFP fluorescence was localized in the endoplasmic reticulum. It can be transiently expressed or stably expressed in plant cells by Agrobacterium transfection. | p35S:ER/CFP | Kan | Hygro/CFP | Inquiry | |
Bp06 | The 35S promoter initiates NES / mCherry gene expression. mCherry is located in the cytoplasm. It can be transiently expressed or stably expressed in plant cells by Agrobacterium transfection. | p35S:NES/mCherry | Kan | Hygro/mCherry | Inquiry | |
Bp07 | 35S initiates target gene expression. The N-terminus of the target gene contains a mitochondrial guidance signal, and the C-terminus is fused with mCherry. It can be transiently expressed, and can also be transfected into plant cells by Agrobacterium for stable expression. | p35S:Mito/MCS/mCherry-p35S:Hygro | Kan | Hygro/mCherry | Inquiry | |
Bp08 | 35S initiates the expression of CFP in the nucleus. The N-terminal of CFP contains NLS signal. It can be transiently expressed, and can also be transfected into plant cells by Agrobacterium for stable expression. | p35S:NLS/CFP | Kan | Neo+/CFP | Inquiry | |
Bp09 | The 35S promoter initiates the expression of the target gene. The N-terminal of the target gene was fused with EGFP protein. It can be transiently expressed, and can also be transfected into plant cells by Agrobacterium for stable expression. | p35S:EGFP/MCS-p35S:Neo | Kan | Neo+ | Inquiry | |
Bp10 | The 35S promoter initiates the GFP signal to localize on the cell membrane. It can be transiently transformed or transfected into plant cells by Agrobacterium for stable expression. | p35S:EGFP/PM-signal | Kan | Neo+/EGFP | Inquiry | |
Bp11 | The 35S promoter initiates the expression of the target gene. The C-terminus of the target gene was fused with YFP. It can be transiently expressed, and can also be transfected into plant cells by Agrobacterium for stable expression. | p35S:MCS/YFP | Kan | Neo+/YFP | Inquiry | |
Bp12 | The 35S promoter initiates the expression of the target gene. The C-terminus of the target gene was fused with mCherry. It can be transiently expressed, and can also be transfected into plant cells by Agrobacterium for stable expression. | p35S:MCS/mCherry | Kan | Neo+/mCherry | Inquiry | |
Bp13 | The 35S promoter initiates the expression of the target gene. The C-terminus of the target gene was fused with CFP. It can be transiently expressed, and can also be transfected into plant cells by Agrobacterium for stable expression. | p36S:MCS/CFP | Kan | Neo+/CFP | Inquiry | |
Bl01 | Lactobacillus / Lactococcus lactis | The p32 promoter initiates target gene expression, constitutive expression, and low-copy vector. | p32:MCS | Ery+/Chloramphenicol | NA | Inquiry |
Bl02 | The PnisA promoter initiates target gene expression. The expression host was NZ9000 Lactococcus lactis. Medium copy expression vector, the vector must use MC1601 competent state in amplification and propagation. | pnis:MCS | Chl+ | NA | Inquiry | |
Bl03 | The p59 promoter initiates the expression of the target gene. It has a secretory signal peptide and nucA, which is conducive to the anchoring of the target protein on the cell surface after secretion, and belongs to the secretory expression vector. | p59:PSusp/nucA-MCS | Amp+ | Ery+ | Inquiry | |
Bi01 | Insect | Polyhedrin promoter initiates target gene expression. The N-terminus of the target gene is connected with the GUS gene. | pPH:GUS-MCS | Amp+ | GUS | Inquiry |
Bi02 | Polyhedrin promoter initiates target gene expression. The N-terminus of the target gene contains a His tag and a TEV site, and the N-terminus is fused with GFP protein. | pPH:6*His-TEV site-MCS/EGFP | Amp+ | EGFP/GmR | Inquiry | |
Bsa01 | Saccharomyces Cerevisiae | The GAL1 promoter induced by galactose initiates the expression of the target gene. The C-terminal of the target gene was fused with GFP protein. | pGAL1:MCS/EGFP | Amp+ | URA3 | Inquiry |
Bst01 | staphylococcus aureus | The Rpsl promoter initiates mCherry expression. | pRpsl:RBS/mCherry | Amp+/Chl+/mCherry | NA | Inquiry |
Vector Number | Species | Functional Description | Name | Prokaryotic Screening Resistance / Marker | Eukaryotic Screening Resistance / Marker | Order |
---|---|---|---|---|---|---|
Cm01 | Mammal | The CMV promoter initiates the expression of the target gene in mammalian cells. There is a T7 promoter sequence at the front end of the target sequence. Non-viral vectors can also be used as in vitro transcription vectors. | pCMV-pT7:MCS | Amp | Hygro | Inquiry |
Cm02 | The CMV promoter initiates the expression of the target gene in mammalian cells. There is a T7 promoter sequence at the front end of the target sequence. Non-viral vectors can also be used as in vitro transcription vectors. | pCMV-pT7:MCS | Amp | NeoR/KanR | Inquiry | |
Cm03 | The CMV promoter initiates the expression of the target gene in mammalian cells. There is a T7 promoter sequence at the front end of the target sequence. Non-viral vectors can also be used as in vitro transcription vectors. | pCMV-pT7:MCS | Amp | NeoR/KanR | Inquiry | |
Cm04 | The CMV promoter initiates the expression of the target gene in mammalian cells. The target sequence has a T7 promoter sequence at the front end, and the C-terminal of the target protein is fused with Myc and 6 * His tags. Non-viral vector. | pCMV-pT7:MCS/Myc/6×His | Amp | NeoR/KanR | Inquiry | |
Cm05 | The CMV promoter initiates the expression of the target gene in mammalian cells. The C-terminal of the target gene is connected with WPRE, and the C-terminal region of WPRE contains a Factor Xa site. Non-viral vector. | pCMV:MCS-WPRE(Factor Xa site) | Amp | NeoR/KanR | Inquiry | |
Cm06 | The CMV promoter initiates the expression of the target gene in mammalian cells, and the C-terminal of the target gene is connected with WPRE. The target sequence can be connected to the carrier by Topo connection method. | pCMV:Topo site-WPRE | Amp | Neo | Inquiry | |
Cm07 | CMV-IE initiates target gene expression. Mammalian high-efficiency expression vector, no eukaryotic screening resistance, no fluorescent gene. Transient expression vector and non-viral vector. | pCMV-IE:MCS | Amp | N/A | Inquiry | |
Cm08 | The CMV promoter initiates the expression of the target gene in mammalian cells. There is a T7 promoter sequence at the front end of the target sequence. Non-viral vectors can also be used as in vitro transcription vectors. | pCMV-pT7:MCS | Amp | Hygro | Inquiry | |
Cm09 | The CMV-IE promoter initiates the expression of the target gene, which is regulated by tetracycline. Non-viral vector. | pCMV-IE:MCS/Tet-On 3G | Amp/Kan | NeoR/KanR | Inquiry | |
Cm10 | The CMV promoter initiates the expression of the target gene in mammalian cells. No eukaryotic screening resistance, no fluorescent tags. Transient expression vector, containing NotI restriction sites. Through this site, the expression cassette can be cut off and subcloned into the ITR-containing vector. | pCMV:MCS | Amp | N/A | Inquiry | |
Cm11 | The CMV promoter initiates the expression of the target gene in mammalian cells. The target sequence has a T7 promoter sequence at the front end, and the C-terminal of the target gene is fused with Myc and 6 * His tags. Non-viral vectors can also be used as in vitro transcription vectors. | pCMV-pT7:MCS/Myc/6×His | Amp/BSD | BSD | Inquiry | |
Cm12 | The CMV promoter initiates the expression of the target gene in mammalian cells. In vitro transcription vector, the sense strand RNA or antisense strand RNA of the target gene was obtained by in vitro transcription under the action of T7 polymerase or SP6 polymerase. Non-viral vector. | pCMV-pT7:MCS-pSP6 | Amp | NeoR/G418 | Inquiry | |
Cm13 | The CMV promoter initiates the expression of the target gene in mammalian cells. There is a T7 promoter sequence at the front end of the target sequence. The C-terminus of the target gene was fused with Myc and Flag tags. It can also be used as an in vitro transcription vector. | pCMV-pT7:MCS/Myc/Flag | Kan | NeoR/G418 | Inquiry | |
Cm14 | The simultaneous expression of two target genes was initiated by two CMV promoters, respectively. Transient expression vector. | pCMV1:MCS1-pCMV2:MCS2 | Amp | N/A | Inquiry | |
Cm15 | Cell surface display carrier. CMV initiates target protein expression. The N-terminus of the target protein contains IgK leader sequence to guide extracellular secretion, and the C-terminus has a membrane localization signal PDGFP, which is located on the cell membrane. | pCMV:IgK leader/MCS/PDGFR | Amp | NeoR | Inquiry | |
Ce01 | E.coli | T7 promoter initiates target gene expression. The T7 tag was fused at the N-terminus of the target gene. Transient expression and constitutive expression. | pT7:T7 tag-MCS | Kan | N/A | Inquiry |
Ce02 | T7 promoter initiates target gene expression. The T7 tag was fused at the N-terminus of the target gene. 6 * His fused to the C-terminus of the target gene. Transient expression and constitutive expression. | pT7:T7 tag/MCS/6*His | Amp | N/A | Inquiry | |
Ce03 | T7 promoter initiates target gene expression. The target gene contains a pelB signal peptide at the N-terminus and a 6 * His at the C-terminus. The target protein is located in periplasm. | pT7:pelB-MCS/6*His | Amp | N/A | Inquiry | |
Ce04 | T7 promoter initiates target gene expression. There is a T7 tag at the N-terminus of the target gene. | pT7:T7 tag/MCS | Amp | N/A | Inquiry | |
Ce05 | T7 promoter initiates target gene expression. The N-terminus of the target gene contains a T7 tag. | pT7:T7 tag/MCS | Amp | N/A | Inquiry | |
Ce06 | T7 promoter initiates target gene expression. The N-terminal of the target gene contains 6 * His tag, T7 tag, Xpress antigen recognition site and EK site in turn. | pT7:6*His/T7 tag/Xpress Epitope/EK/MCS | Amp | N/A | Inquiry | |
Ce07 | Two T7lac promoters were induced by IPTG to initiate the simultaneous expression of the two target genes. One of the target genes was fused with the 8 * His tag at the N-terminus, and the other target gene was fused with the S-Tag tag at the C-terminus. Two target gene co-expression vectors, transient expression and non-viral vectors. |
pT7lac:8*His/MCS1-pT7lac:MCS2/S-Tag; 含有placI:lacI |
CmR | N/A | Inquiry | |
Ce08 | In the cold environment, IPTG was applied to induce the expression of the target gene. The N-terminus of the target gene was fused with a 6 × His tag. Transient expression, non-viral vector. | pcspAlac:6×His/MCS | Amp | N/A | Inquiry | |
Ce09 | IPTG was applied to induce T7 promoter to initiate target gene expression. The N-terminus of the target gene has a 6 * His Tag, and there is a Thrombin cleavage site between the 6 * His tag and the target gene. High expression vector, transient expression and constitutive expression. | pT7lac:6*His-a thrombin site-MCS | Amp | N/A | Inquiry | |
Ce10 | IPTG was applied to induce T7 promoter to initiate target gene expression. The N-terminal of the target gene has a 10 * His Tag, and there is an enterokinase cleavage site between the 10 * His tag and the target gene. High expression vector, transient expression and constitutive expression. | pT7lac:10*His-a enterokinase site-MCS | Amp | N/A | Inquiry | |
Ce11 | IPTG was applied to induce T7 promoter to initiate target gene expression. The target gene contains a T7 tag at the N-terminus and a 6 * His tag at the C-terminus. | pT7lac:T7 tag/MCS/6*His | Amp | N/A | Inquiry | |
Ce12 | IPTG was applied to induce T7 promoter to initiate target gene expression. The target gene contains a T7 tag at the N-terminus and a 6 * His tag at the C-terminus. | pT7lac:T7 tag/MCS/6*His | Amp | N/A | Inquiry | |
Ce13 | IPTG was applied to induce T7 promoter to initiate target gene expression. The N-terminus of the target gene contains a pelB signal peptide, which makes the target protein localized to the periplasm. The C-terminal of the target protein contains a 6 * His tag. | pT7lac:pelB/MCS/6*His | Amp | N/A | Inquiry | |
Ce14 | IPTG was applied to induce T7 promoter to initiate target gene expression. The target gene contains a T7 tag at the N-terminus and a 6 * His tag at the C-terminus. | pT7lac:T7 tag/MCS/6*His | Kan | N/A | Inquiry | |
Ce15 | T7 promoter initiates target gene expression. The target gene contains a pelB signal peptide at the N-terminus and a 6 * His at the C-terminus. The target protein is located in periplasm. | pT7lac:pelB/MCS/6*His | Kan | N/A | Inquiry | |
Ce16 | IPTG was applied to induce T7 promoter to initiate target gene expression. The N-terminal of the target gene contains 6 * His, T7 tag, and the C-terminal contains 6 * His tag. There is a thrombin cleavage site between the N-terminal 6 * His and the T7 tag. | pT7lac:6*His/a thrombin site/T7 tag/MCS/6*His | Kan | N/A | Inquiry | |
Ce17 | IPTG was applied to induce T7 promoter to initiate target gene expression. The N-terminal of the target gene contains 6 * His, T7 tag, and the C-terminal contains 6 * His tag. There is a thrombin cleavage site between the N-terminal 6 * His and the T7 tag. | pT7lac:6*His/a thrombin site/T7 tag/MCS/6*His | Kan | N/A | Inquiry | |
Ce18 | IPTG was applied to induce T7 promoter to initiate target gene expression. The target gene contains an S tag at the N-terminus and a 6 * His tag at the C-terminus. There is a thrombin cleavage site between the S tag and the target gene. | T7lac:S Tag/a thrombin site/MCS/6*His | Kan | N/A | Inquiry | |
Ce19 | IPTG was applied to induce T7 promoter to initiate target gene expression. The N-terminal of the target gene contains a 6 * His tag, the S-tag, and the C-terminal contains a 6 * His tag. There is a thrombin site between 6 * His and S-tag. There is an enterokinase cleavage site between the S-tag and the target gene. | pT7lac:6*His/a thrombin site/S tag/enterokinase site/MCS/6*His | Kan | N/A | Inquiry | |
Ce20 | IPTG was applied to induce T7 promoter to initiate target gene expression. The N-terminal of the target gene contains a 6 * His tag, the S-tag, and the C-terminal contains a 6 * His tag. There is a thrombin site between 6 * His and S-tag. There is an enterokinase cleavage site between the S-tag and the target gene. | pT7lac:6*His/a thrombin site/S tag/enterokinase site/MCS/6*His | Kan | N/A | Inquiry | |
Ce21 | IPTG was applied to induce T7 promoter to initiate target gene expression. The N-terminus of the target gene contains TrxA ( thioredoxin A ) to promote soluble protein expression, 6 * His tag and S-tag. The C-terminus contains a 6 * His tag. There is a thrombin site between the N-terminal 6 * His tag and the S tag. There is an enterokinase site between the S-tag and the target gene. | pT7lac:TrxA-6*His/a thrombin site/S-Tag/enterokinase site/MCS/6*His | Amp | N/A | Inquiry | |
Ce22 | IPTG was applied to induce T7 promoter to initiate target gene expression. The N-terminus of the target gene contains DsbC ( disulfide isomerase ) to promote soluble protein expression, 6 * His tag and S-tag. The C-terminus contains an 8 * His tag. There is a thrombin site between the N-terminal 6 * His tag and the S tag. There is an enterokinase site between the S-tag and the target gene. | pT7lac:DsbC/6*His/a thrombin site/S-Tag/enterokinase site/MCS/8*His | Kan | N/A | Inquiry | |
Ce23 | IPTG was applied to induce T7 promoter to initiate target gene expression. The N-terminus of the target gene contains GST tag, 6 * His and S-Tag. The C-terminus contains an 8 * His tag. There is a thrombin site between the N-terminal 6 * His and S-Tag. There is an enterokinase site between the S-tag and the target gene. | pT7lac:GST/6*His/a thrombin site/S-Tag/enterokinase site/MCS/8*His | Kan | N/A | Inquiry | |
Ce24 | IPTG was applied to induce T7 promoter to initiate target gene expression. The N-terminus of the target gene contains a 6 * His tag and the C-terminus contains an S-tag tag. There is an enterokinase site between the target gene and 6 * His. | pT7lac:6*His/enterokinase site/MCS/S-Tag | Amp | N/A | Inquiry | |
Ce25 | IPTG was applied to induce T7 promoter to initiate target gene expression. The C-terminus of the target gene contains a 6 * His tag. | pT7lac:MCS/6*His | Amp | N/A | Inquiry | |
Ce26 | IPTG was applied to induce the simultaneous expression of two target genes by two T7 promoters. The N-terminal of one target gene contains a 6 * His tag, and the C-terminal of the other target gene contains a S Tag tag. | pT7lac:6*His/MCS-pT7lac:MCS/S-Tag | Amp | N/A | Inquiry | |
Ce27 | IPTG was applied to induce T7 promoter to initiate target gene expression. The N-terminal and C-terminal of the target gene have a 6 * His tag, respectively. | pT7lac:6*His/MCS/6*His | Kan | N/A | Inquiry | |
Ce28 | IPTG was applied to induce T7 promoter to initiate target gene expression. The C-terminus of the target gene was fused with Mxe intein and CBD. The fusion protein could bind to chitin (CBD) magnetic beads. Thiol-induced Mxe intein cleavage activity releases the target protein. | pT7lac:MCS/Mxe intein/CBD | Amp | N/A | Inquiry | |
Ce29 | IPTG was applied to induce T7 promoter to initiate target gene expression. The N-terminal of the target gene contains 6 * His tag, 6 * His tag and S-Tag tag in turn. There is a NusA between the two 6 * His tags to help the target protein soluble. There is a thrombin cleavage site and an enterokinase site between S-Tag and the target gene. The C-terminus of the target gene contains a HSV tag and a 6 * His tag. | pT7lac:6*His/NusA/6*His/S-Tag/a thrombin site/enterokinase site/MCS/HSV tag/6*His | Amp | N/A | Inquiry | |
Ce30 | IPTG was applied to induce T7 promoter to initiate target gene expression. The N-terminus of the target gene contains a 6 * His tag, a thrombin site, and a SUMO tag. SUMO tag can promote the solubility of the target protein. As a chaperone protein, it can promote the correct folding of protein and enhance the tolerance of target protein to heat and protease. It can also shield the toxicity of some toxic proteins to host bacteria. The C-terminus of the target protein contains a 6 * His tag. | pT7lac:6*His/thrombin site/SUMO/MCS/6*His | Kan | N/A | Inquiry | |
Ce31 | IPTG was applied to induce T5 promoter to initiate target gene expression. The C-terminus of the target gene contains a 6 * His tag. | pT5lac:MCS/6*His | Amp | N/A | Inquiry | |
Ce32 | IPTG was applied to induce T5 promoter to initiate target gene expression. The N-terminus of the target gene contains a 6 * His tag. Low copy vector. | pT5lac:6*His/MCS | Amp | N/A | Inquiry | |
Ce33 | IPTG was applied to induce the tac promoter to initiate the target gene expression. Low expression can reduce the probability of forming inclusion bodies, low copy vector. | ptac:MCS | Amp | N/A | Inquiry | |
Ce34 | IPTG was applied to induce the tac promoter to initiate the target gene expression. The N-terminus of the target gene contains MBP to localize the protein in the periplasm. There is a Factor Xa site between the target gene and MBP. | ptac:MBP/Factor Xa site/MCS | Amp | N/A | Inquiry | |
Ce35 | IPTG was applied to induce the tac promoter to initiate the target gene expression. The N-terminus of the target gene contains MBP to localize the protein in the periplasm. There is a Factor Xa site between the target gene and MBP. | ptac:MBP/Factor Xa site/MCS | Amp | N/A | Inquiry | |
Ce36 | IPTG was applied to induce the CSPA promoter to initiate the target gene expression under cold environment treatment. The N-terminus of the target gene contains a TEE tag. | pCSPAlac:TEE/MCS | Amp | N/A | Inquiry | |
Ce37 | Application of arabinose induced araBAD promoter to initiate target gene expression. The N-terminus of the target gene contains a 6 * His tag. In glucose-free medium, arabinose positively regulates the expression of target genes. The soluble expression of the target protein was optimized by adjusting the concentration level of arabinose. | pBAD:MCS/Myc/6*His | Amp | N/A | Inquiry | |
Ce38 | Lac initiates the expression of Trc promoter. The N-terminus of the target protein contains 6 His purification tags for protein purification. There is a rTEV protease recognition site between the His tag and the target protein, which can be digested by rTEV protease to remove the fused His tag. | pLacTrc:6*His-rTEV protease cleavage site-MCS | Amp | N/A | Inquiry | |
Cpy01 | Yeast | Pichia pastoris protein expression vector and integrated vector. GAP promoter initiates target gene expression. The N-terminus of the target gene contains an α-factor secretion signal peptide to secrete the target protein. The C-terminus of the target gene contains a Myc tag and a 6 * His tag. | pGAP:α factor/MCS/Myc/6*His | Zeo | Zeo | Inquiry |
Cpy02 | Pichia pastoris expression vector. The AOX1 promoter initiates target gene expression. The N-terminus of the target gene contains an α-factor secretion signal peptide to secrete the target protein. | pAOX1:α factor/MCS | Amp | Kan/HIS4 | Inquiry | |
Cpy03 | Pichia pastoris expression vector. The AOX1 promoter initiates target gene expression. The N-terminus of the target gene contains an α-factor secretion signal peptide to secrete the target protein. The C-terminus of the target gene contains a Myc tag and a 6 * His tag. | pAOX1:α factor/MCS/Myc/6*His | Zeo | Zeo | Inquiry | |
Csa01 | Saccharomyces cerevisiae expression vector. Galactose induced the GAL1 promoter to initiate the expression of the target gene at a high level, and glucose inhibited the expression. | pGAL1-pT7:MCS | Amp | URA3 | Inquiry | |
Csa02 | Saccharomyces cerevisiae surface display vector. GAL1 initiates the expression of AGA2 expression cassette. The target protein is inserted into the AGA2 expression box and needs to be expressed in Saccharomyces cerevisiae containing Aga1 to achieve surface display. | pGAL1-pT7:AGA2/GS linker Xpress/MCS/V5 epitope 6*His | Amp | TRP1 | Inquiry | |
Ci01 | Insect | Baculovirus expression system vector. There are two promoters of P10 and Polyhedrin on the vector, which respectively initiate the expression of the target gene and realize the simultaneous expression of the two genes on a single vector. | pP10:MCS-pPH:MCS | Amp | Gent | Inquiry |
Ci02 | Polyhedrin promoter initiates target gene expression. The N-terminus of the target gene contains a 6 * His tag. There is a TEV cleavage recognition site between the target gene and the 6 * His tag. | pPH:6*His/TEV/MCS | Amp | Gent | Inquiry | |
Ci03 | Polyhedrin promoter initiates target gene expression. The N-terminus of the target gene contains a 6 * His tag. There is a TEV cleavage recognition site between the target gene and the 6 * His tag. | pPH:6*His/TEV/MCS | Amp | Gent | Inquiry | |
Ci04 | Polyhedrin promoter initiates target gene expression. The N-terminus of the target gene contains a 6 * His tag. There is a TEV cleavage recognition site between the target gene and the 6 * His tag. The C-terminus of the target gene contains a Flag tag. | pPH:6*His/TEV/MCS/Flag | Amp | Gent | Inquiry | |
Ci05 | Polyhedrin promoter initiates target gene expression. No fusion protein tag. The target sequence needs to contain the start codon and the stop codon. Insect expression vector and baculovirus expression vector. | pPH:MCS | Amp | Gent | Inquiry | |
Cb01 | bacillus subtilis | Grac induced by IPTG initiates target gene expression. Highly efficient expression of recombinant foreign protein in Bacillus subtilis. | pGracLac:MCS | Amp/Chloramphenicol | N/A | Inquiry |
Cb02 | Grac induced by IPTG initiates the expression of the target gene and can secrete and express the recombinant protein at a high level. The pGrac sequence contains the SD sequence and the lacO operator sequence. | pGracLac:SamyQ/MCS | Amp/Chloramphenicol | N/A | Inquiry | |
Csal01 | salmonella | GFP was induced and mCherry was constitutively expressed. | pBAD:GFP-prpsM:mCherry | Amp/mCherry/GFP | N/A | Inquiry |
Expression Regulatory Vectors
Vector Number | Species | Functional Description | Name |
Prokaryotic Screening Resistance / Marker |
Eukaryotic Screening Resistance / Marker |
Order |
---|---|---|---|---|---|---|
GAm01 | Mammal | The promoter EF-1α initiates dCas9-VP64-2A-Blast expression. Lentivirus packaging and Blast screening resistance can be performed. SAM activation system, three-vector system (vector 1). | pEF-1α:dCas9/VP64/Blast | Amp | Blast | Inquiry |
GAm02 | The promoter EF-1α initiates the expression of dCas9-VP64 / EGFP. Lentivirus packaging and EGFP fluorescence screening can be performed. SAM activation system, three-vector system (vector 1). | pEF-1α:dCas9/VP64/EGFP | Amp | EGFP | Inquiry | |
GAm03 | The promoter EF-1α initiates the expression of the activation element MS2-P65-HSF1, which can be used for lentivirus packaging and Hygro screening resistance. SAM activation system, three-vector system (vector 2). | pEF-1α:MS2-P65-HSF1 | Amp | Hygro | Inquiry | |
GAm04 | The promoter U6 initiates the expression of sgRNA and regulatory element MS2 stem loop. Lentivirus packaging and TagBFP fluorescence screening can be performed. SAM activation system, three-vector system (vector 3). | pU6:sgRNA-MS2 stem loop | Amp | Puro/TagBFP | Inquiry | |
GAm05 | Promoter U6 initiates sgRNA / MS2 stem loop expression. The promoter EF-1α initiates dCas9 / VP64 expression. Puro screening resistance. SAM dual-vector activation system (vector 1). Lentivirus packaging can be performed. | pU6:sgRNA/MS2 stem loop-pEF-1α:dCas9-VP64 | Amp | Puro | Inquiry | |
GAm06 | The promoter EF-1α initiates the expression of the activation element MS2-P65-HSF1. Lentivirus packaging can be performed. Neo / G418 screening resistance. SAM activation system, double vector system (vector 2). | pEF-1α:MS2-P65-HSF1 | Amp | Neo/G418 | Inquiry | |
GAm07 | All-in-one CRISPRa vector (single plasmid system). Lentivirus packaging can be performed. SAM activation system, single vector system, screened by Blast. | pU6:sgRNA(MS2)-pEF-1α:dCas9/VP64-p65-HSF1/Blast | Amp/BleoR | Blast | Inquiry | |
GAm08 | The promoter EF-1α initiates dCas9-VPR / EGFP expression. Lentivirus packaging and EGFP fluorescence screening tags can be performed. VPR dual-vector activation system (vector 1). A complete VPR activation system is formed with a gRNA vector. | pEF-1a:dCas9-VPR/EGFP | Amp | EGFP | Inquiry | |
GAm09 | The promoter EF-1a initiates dCas12a-VPR / Puro expression. Lentivirus packaging and Puro screening tags can be performed. VPR dual-vector activation system (vector 1). A complete VPR activation system is formed with a gRNA vector. | pEF-1a:dCas12a-VPR/Puro | Amp | Puro | Inquiry | |
GAm10 | The promoter EF-1α initiates the expression of SaCas9-VPR, contains gRNAscaffold, and can insert sgRNA promoter in the MCS region. Lentivirus packaging and Puro screening can be performed. VPR single vector activation system. | MCS:sgRNA-pEF-1α:SaCas9-VPR | Amp/BleoR | Puro | Inquiry | |
GIm01 | Mammal | RNAi regulation efficiency detection vector. The T7 promoter initiates the expression of the target gene, and the N-terminal of the target gene contains hRluc protein. The target gene was cloned to the multiple cloning site downstream of the renilla luciferase translation termination codon. RNAi of the target gene, initiated by synthetic siRNA or in vivo expressed shRNA, leads to cleavage and subsequent degradation of the fused mRNA. Detecting the decrease of renilla luciferase activity is a simple method to monitor the effect of RNAi. | pT7:hRluc/MCS-pHS-TK:hluc+ | Amp | hRluc/hluc+ | Inquiry |
GIm02 | N/A | N/A | N/A | N/A | Inquiry | |
GIm03 | CRISPR inhibition vector. All-in-one vector. The UbC promoter initiates dCas9 and the inhibitory element KRAB expression, and the U6 promoter initiates sgRNA expression. Lentivirus packaging can be performed. | pUbC:dCas9/KRAB-pU6:sgRNA | Amp | Puro | Inquiry | |
GIm04 | CRISPR sgRNA vector, which is suitable for CRISPR inhibition or activation of dual vector system, can be used for lentivirus packaging. The vector does not contain Cas9 protein, and needs to complete the activation or inhibition function together with the vector containing Cas9 and activation or inhibition elements. | pmU6:sgRNA-pEF1a:Puro/TagBFP | Amp | Puro/BFP | Inquiry | |
GIm05 | The CRISPR double vector inhibition system, dCas9-KRAB vector, no sgRNA, and sgRNA vector constitute the inhibition system. Lentivirus packaging can be performed. | pSFFV:dCas9-TagBFP-KRAB | Amp | TagBFP | Inquiry | |
GIm06 | CRISPR dual vector inhibition system, KRAB-dCas9 vector, no sgRNA, and sgRNA vector form an inhibition system. Lentivirus packaging can be performed. | pSFFV:KRAB-dCas9/mCherry | Amp | mCherry | Inquiry | |
GIm07 | RNAi expression vector, siRNA expression vector, transient expression vector. H1 promoter initiates the expression of target siRNA. | pH1:MCS | Amp | N/A | Inquiry | |
GIm08 | RNAi expression vector, siRNA expression vector. H1 promoter initiates the expression of target siRNA. | pH1:MCS | Amp | Hygro | Inquiry | |
GIm09 | RNAi expression vector, siRNA expression vector. CMV promoter initiates the expression of target siRNA. | pCMV:MCS | Amp | Neo | Inquiry | |
GIm10 | RNAi vector, siRNA expression vector, low-copy vector. Lentivirus packaging can be performed. | pU6:MCS | Amp | Puro | Inquiry | |
GIm11 | RNAi vector, tetracycline-induced shRNA expression vector. | pTightU6:MCS | Amp | Neo | Inquiry | |
GIm12 | RNAi vector, transient expression vector. H1 promoter initiates RNA expression. | pH1:MCS-pPGK:EGFP/Neo | Amp | Neo/EGFP | Inquiry | |
GIm13 | RNAi vector, shRNA expression vector. Lentivirus packaging can be performed. H1 promoter initiates the expression of target shRNA. | pH1:MCS-pEF1a:EGFP | Amp | EGFP | Inquiry | |
GIm14 | RNAi vector, shRNA expression vector. Lentivirus packaging can be performed. U6 promoter initiates target gene expression. The target sequence expression can be achieved by replacing the stuffer region in the vector with the target sequence. | pU6:Stuffer-phPGK:Puro | Amp | Puro | Inquiry | |
GIm15 | RNAi vector, shRNA expression vector. Lentivirus packaging can be performed. U6 promoter initiates target gene expression. CMV promoter initiates EGFP expression. hPGK initiates Puro expression. | pU6:MCS-pCMV:EGFP-phPGK:Puro | Amp | Puro/EGFP | Inquiry | |
GIp01 | Plant | RNAi vector. 35S initiates RNA expression. It can be transiently expressed or stably expressed. | p35S:MCS1-chsA intron-MCS2 | Kan | Bar+ | Inquiry |
GIp02 | RNAi vector, transient expression vector. 35S initiates RNA expression. | p35S:MCS1-PDK intron-MCS2 | Amp | N/A | Inquiry | |
GIp03 | RNAi vector, transient expression vector. 35S initiates RNA expression. | p35S:MCS1-PDK intron-MCS2 | Kan | N/A | Inquiry | |
GIp04 | RNAi vector. UBi-1 initiates RNA expression. It can be transiently expressed or stably expressed. | pUBi-1:MCS1-rice intron-MCS2 | Kan | Hygro/GUS | Inquiry | |
GInem01 | Nematodes | RNAi vector, transient expression vector. Two T7 promoters bidirectionally initiate RNA expression. | pT7:MCS1-MCS2:pT7 | Amp | N/A | Inquiry |
GIfu01 | Fungi | RNAi vector. TrpC promoter initiates RNA expression. It can be transiently expressed or stably expressed. | pTrpC:MCS1-IT-MCS2 | Amp | Hygro | Inquiry |
In Vitro Transcription Vectors
Vector Number | Species | Functional Description | Name |
Prokaryotic Screening Resistance / Marker |
Eukaryotic Screening Resistance / Marker |
Order |
---|---|---|---|---|---|---|
H01 | N/A | mRNA in vitro transcription vector. The vector contains a polyA sequence. The target sequence is added in front of the polyA sequence (such as the BbsI restriction site). The target sequence should contain T7 promoter, 5'UTR, and 3'UTR, while the BspQI restriction site should be avoided in the target sequence. | MCS:polyA | Kan+ | N/A | Inquiry |
H02 | N/A | mRNA in vitro transcription vector. The vector contains a polyA sequence. The target sequence is added in front of the polyA sequence (such as the BbsI restriction site). The target sequence of the vector should contain T7 promoter, 5'UTR, and 3'UTR, while the MluI restriction site should be avoided in the target sequence. | MCS:polyA | Kan+ | N/A | Inquiry |
H03 | Mammal | mRNA in vitro transcription vector and transient expression vector. It contains T7 promoter, 5'UTR, and 3'UTR sequences suitable for mammalian cells (human, mouse), and contains a polyA sequence. The target gene sequence is inserted at the EcoRI site, and the HA tag sequence is contained behind the EcoRI site, which can be used to label the target protein. | pCMV/pT7:5'UTR-EcoRI-HA-3’UTR-polyA | Kan+ | N/A | Inquiry |
H04 | Mammal | mRNA in vitro transcription vector and transient expression vector. It contains T7 promoter, 5'UTR, and 3'UTR sequences suitable for mammalian cells (human, mouse), and contains a polyA sequence. The target gene sequence is inserted into the EcoRI site, which contains the HA tag sequence and the EGFP sequence after the EcoRI site, which can be used to mark the target protein. | pCMV/pT7:5'UTR-EcoRI-HA/EGFP-3’UTR-polyA | Kan+ | N/A | Inquiry |
Editing Vectors
Vector Number | Species | Functional Description | Name |
Prokaryotic Screening Resistance / Marker |
Eukaryotic Screening Resistance / Marker |
Order |
---|---|---|---|---|---|---|
Em01 | Mammal | All-in-one vector. The EF1α promoter initiates Cas9 expression, the U6 promoter initiates sgRNA expression, and is screened with Puro. Lentivirus packaging can be performed. | pU6:sgRNA-pEF1a:Cas9 | Amp | Puro | Inquiry |
Em02 | All-in-one vector. The EF1α promoter initiates Cas9 expression, the U6 promoter initiates sgRNA expression, and GFP is used for screening. Lentivirus packaging can be performed. | pU6 :sgRNA-pEF1a:Cas9 | Amp | EGFP | Inquiry | |
Em03 | All-in-one vector. EF1αpromoter initiates Cas9 expression and U6 promoter initiates sgRNA expression, which can be screened by mCherry and Puro. Lentivirus packaging can be performed. | pU6:sgRNA-pEF1a:Cas9 | Amp | Puro/mCherry | Inquiry | |
Em04 | Cas9 vector. The EF1α promoter initiates Cas9 expression. Lentivirus packaging can be performed. | pEF1a:Cas9 | Amp | Blast | Inquiry | |
Em05 | Cas9 vector. Tetracycline induced Tight Tre promoter to initiate Cas9 expression. Blast can be used for screening. Lentivirus packaging can be performed. | pTight Tre:Cas9 | Amp | Blast | Inquiry | |
Em06 | sgRNA vector. U6 promoter initiates sgRNA expression. Puro can be used for screening. Lentivirus packaging can be performed. | pU6:sgRNA | Amp | Puro | Inquiry | |
Em07 | sgRNA vector. U6 promoter initiates sgRNA expression. Puro and DsRed can be used for screening. Lentivirus packaging can be performed. | pU6:sgRNA | Amp | Puro/DsRed | Inquiry | |
Em08 | sgRNA vector. U6 promoter initiates sgRNA expression. Puro and GFP can be used for screening. Lentivirus packaging can be performed. | pU6:sgRNA | Amp | Puro/EGFP | Inquiry | |
Em09 | sgRNA vector, single cell sequencing vector. U6 promoter initiates sgRNA expression. Puro can be used for screening. Lentivirus packaging can be performed. | pU6:sgRNA | Amp | Puro | Inquiry | |
Em10 | All-in-one vector. The CMV promoter initiates SaCas9 expression, and the U6 promoter initiates Sa-gRNA expression. No screening label. Adeno-associated virus packaging can be performed. | pCMV:SaCas9-pU6:Sa-gRNA | Amp | N/A | Inquiry | |
Em11 | All-in-one vector. The CMV promoter initiates SaCas9 expression, and the U6 promoter initiates Sa-gRNA expression. You can use mCherry to filter. Adeno-associated virus packaging can be performed. | pCMV:SaCas9-pU6:Sa-gRNA | Amp | mCherry | Inquiry | |
Em12 | Sa-sgRNA vector. U6 promoter initiates sa-gRNA expression, and CMV initiates EGFP expression. EGFP can be used for screening. AAV packaging can be carried out. | pU6:sa-gRNA-pCMV:EGFP | Amp | EGFP | Inquiry | |
Em13 | Sp-sgRNA vector. U6 promoter initiates sp-gRNA expression, and hSyn initiates EGFP expression. EGFP can be used for screening. AAV packaging can be carried out. | pU6:sp-gRNA-phSyn:EGFP | N/A | N/A | Inquiry | |
Em14 | All-in-one vector, double sgRNA vector. Two U6 promoters are used to initiate the simultaneous expression of two sgRNAs, and the Cbh promoter is used to initiate Cas9 expression, which is a non-viral vector. | pU6:sgRNA-pU6:sgRNA-pCbh:Cas9 | Amp | N/A | Inquiry | |
Em15 | Cas9 vector. The Cbh promoter initiates Cas9 expression. Puro can be used for screening, non-viral vector. | pCbh:Cas9 | Amp | Puro | Inquiry | |
Em16 | Cas9 vector. The Cbh promoter initiates Cas9 expression. GFP can be used for screening, non-viral vector. | pCbh:pCas9 | Amp | GFP | Inquiry | |
Em17 | sgRNA vector. U6 promoter initiates sgRNA expression. Puro can be used for screening, non-viral vector. | pU6:sgRNA | Amp | Puro/Fluc | Inquiry | |
Em18 | CRISPR-AsCas12a All-in-one vector. The EF1a promoter initiates AsCpf1 (AsCas12a) expression, and the U6 promoter initiates crRNA (gScaffold-gRNA) expression. Lentivirus packaging can be performed. | pEF1α:AsCpf1/Puro-pU6:crRNA | Amp | N/A | Inquiry | |
Em19 | The cytosine base editor CBE3, U6 promoter initiates sgRNA expression, and the EF-1α promoter initiates BE3 expression (including APOBEC-1 / nCas9 (D10A) / UGI). The C corresponding to the 4-8 base of the sgRNA sequence is the target mutation base. Lentivirus packaging can be performed. | pU6:sgRNA-pEF-1α:APOBEC-1/nCas9/UGI | Amp | Puro | Inquiry | |
Em20 | The cytosine base editor CBE4, U6 promoter initiates sgRNA expression, and the EF-1α promoter initiates BE4 expression (including APOBEC-1 / nCas9 (D10A) / 2 * UGI). The C corresponding to the 4-8 base of the sgRNA sequence is the target mutation base. Lentivirus packaging can be performed. | pU6:sgRNA-pEF-1α:APOBEC-1/nCas9/2*UGI | Amp | Puro | Inquiry | |
Em21 | Adenine base editor ABE. The CMV promoter initiates the expression of ABE7.10 (TadA-linker-Tad * A (7.10) -linker-nCas9). The editing efficiency in human cells is about 50 % by editing the 4-9th position of the sgRNA sequence. No sgRNA insertion site, non-viral vector. | pCMV:ABE7.10 | Amp | N/A | Inquiry | |
Em22 | Adenine base editor ABE. The U6 promoter initiates sgRNA expression, and the CAG promoter initiates ABE7.10 / St1Cas9 expression. The 4-9 A of sgRNA sequence is edited, and the editing efficiency in human cells is about 50%. Non-viral vector. | pU6:sgRNA-pCAG:ABE7.10/St1Cas9 | Amp | N/A | Inquiry | |
Epara01 | Arabidopsis Thaliana | All-in-one vector. The 35S promoter initiates Cas9 expression, and the At U6-26 promoter initiates sgRNA expression. Hygro screening can be performed. | 35s:Cas9-AtU6-26:sgRNA | Kan | Hygro | Inquiry |
Epri01 | Rice | All-in-one vector. The rice snoRNA U3 promoter initiates sgRNA expression, and the UBI promoter initiates Cas9 expression. Hygro screening can be performed. | rice snoRNA U3 promoter :sgRNA- pUBI:Cas9 | Kan | Hygro | Inquiry |
Ee01 | E. coli | sgRNA vector. It is used for gene knockdown in bacteria, together with the Cas9 vector and donor template DNA. | J23119(SpeI):sgRNA | Amp | N/A | Inquiry |
Ee02 | sgRNA vector. It is used for gene knockdown in bacteria, together with the Cas9 vector and donor template DNA. | J23119(SpeI):sgRNA | Spec | N/A | Inquiry | |
Ee03 | spCas9 vector. Tetracycline-induced spCas9 expression is used for gene knockdown in bacteria. Co-used with Cas9 vector and Donor template DNA. | pLtetO-1:SpCas9 | Amp | N/A | Inquiry | |
Enem01 | Nematodes | sgRNA vector. U6 promoter initiates sgRNA expression. | pU6:sgRNA-DHFR | Amp | DHFR | Inquiry |
Enem02 | sgRNA vector. The T7 promoter initiates in vitro transcription of sgRNA to obtain sgRNA. | pT7:sgRNA | Kan | Neo | Inquiry | |
Enem03 | All-in-one vector. The U6 promoter initiates sgRNA expression, and pTgTUB1 initiates Cas9 expression. | pU6:sgRNA-pTgTUB1:Cas9 | Amp | N/A | Inquiry | |
Enem04 | Cas9 vector. The TgTUB1 promoter initiates Cas9 expression. | pTgTUB1:Cas9 | Amp | CAT | Inquiry | |
Ezeb01 | Zebrafish | Cas9 vector. Non-viral vector. | pCMV-pT7:Cas9/3*Flag | Amp | 3*Flag | Inquiry |
Ezeb02 | sgRNA vector. The gRNA is obtained by in vitro transcription under the T7 promoter. | pT7:sgRNA | Kan | N/A | Inquiry | |
Ezeb03 | All-in-one vector, tissue-specific knockout vector, double sgRNA vector, non-viral vector. | pUAS:Cas9-pU6:sgRNA1;pU6:sgRNA2 | Amp | Cre | Inquiry | |
Edro01 |
Drosophila melanogaster |
All-in-one vector, non-viral vector. dU6-2 initiates sgRNA expression, and Ac5 promoter initiates Cas9 expression. Puro can be used for screening. | pdU6-2:sgRNA-pAc5:Cas9 | Amp | Puro | Inquiry |
Edro02 | sgRNA vector. dU6-2 initiates sgRNA expression, and Ac5 promoter initiates EGFP and Puro expression. EGFP and Puro can be used for screening. | pdU6-2:sgRNA-pAc5:EGFP/Puro | Amp | Puro/EGFP | Inquiry | |
Edro03 | Cas9 vector. MT promoter initiates Cas9 expression. Screening with Hygro. | pMT:Cas9 | Amp | Hygro | Inquiry | |
Esa01 | Saccharomyces cerevisiae | All-in-one vector. The promoter ROX3 initiates spCas9 expression, and the promoter SNR52 initiates sgRNA expression. Neo / G418 can be used for screening. | pROX3:Cas9-pSNR52:sgRNA | Amp | Neo/G418 | Inquiry |
Standard Human CRISPR Libraries
Standard Mouse CRISPR Libraries
Standard Poultry CRISPR Libraries
Standard Animal CRISPR Libraries
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