JM109(DE3) serves as a versatile “all‑in‑one” testing platform:
It combines rapid, high‑quality plasmid preparation (via anti‑rop modified JM109) with direct small‑scale expression screening in the same strain.
This allows early verification of protein expression (soluble/inclusion body) and condition optimization without switching strains.
Compared to BL21(DE3):
BL21(DE3) is protease‑deficient (lon/ompT) and ideal for large‑scale production.
JM109(DE3) acts as a frontline tester, ensuring plasmid integrity and expression feasibility before scaling up, saving time and reducing risk.
Auto‑induction enables true “set‑and‑forget” expression:
No need to monitor OD or add IPTG manually.
Simply inoculate, incubate overnight, and harvest the next morning.
Ideal for high‑throughput screening, toxic proteins (gentler induction), and fast experimental iteration
DNA Synthesis
Vector Selection
Molecular Biology
Oligo Synthesis
RNA Synthesis
Variant Libraries
Genome KO Library
Oligo Pools
Virus Packaging
Gene Editing
Protein Expression
Antibody Services
Peptide Services
DNA Data Storage
Standard Oligo
Standard Genome KO Libraries
Standard Genome Editing Plasmid
ProXpress
Protein Products
