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Antibody Humanization

Synbio Technologies has developed an integrated antibody humanization and affinity maturation platform based on the advanced phage/yeast display technologies and progressive computer modeling and simulation techniques. Our antibody humanization services include in silico CDR-grafting, computer-aided design and optimization, high-throughput screening of phage-display antibodies, and Biacore surface resonance analysis. With this approach, we are able to provide humanized antibodies with diminished immunogenicity, which have comparable or better specificity, bioactivity, thermos-stability, and productivity than parental antibodies.

Highlights

Extensive Experience

Team with practical experience with humanized antibody design, screening, and production to ensure the success of customers’ projects
Effective in Silico Design

Effective and reliable antibody modeling and simulations from multiple antibody databases
High-Throughput Scale

High throughput Phage/yeast display designations enhances the yield of valid antibodies
Guaranteed Affinity

Affinity of final construct guaranteed to be within three-fold

Service Details

Details	Deliverables	Turnaround Time
• Chimeric antibody construction • Antigen-antibody binding analysis	1. 4-5 humanized antibody VH/VL variants and corresponding Fv-DNA sequences. 2.At least 1 variant with binding affinity comparable to parental antibody. 3.Full report (PDF)	4-5 Months
• Antibody model library construction • Phage/Yeast display antibody library construction
• High-throughput antibody screening
• Humanized antibody characterization and production

QUOTE

Case Study

Through our antibody humanization design platform, 25 humanized antibodies were designed and screened. ELISA testing was used to screen four antibodies (H5L4, H6L4, H5L6 and H7L6) for the next affinity test, and the affinity of the humanized antibodies was comparable to that of the control antibody H1L1.

FAQs

Why should we choose humanize antibodies?

Humanizing antibodies reduces immunogenicity, maintains affinity and specificity, and improves safety and efficacy in clinical applications.

What are the most common methods of antibody humanization?

1.Humanization based on framework region homology CDR transplantation with revertant mutations

2.Germline gene-based CDR transfer

3.Antibody Surface Remodeling

4.SDR transplantation

What are the analysis algorithms for humanizing antibody sequences?

H-score: calculates the average sequence identity of a given antibody sequence compared to a small database of human variable region sequences

Germinality index: identifies the proportion of framework residues that are identical between a given antibody variable region sequence and the closest related human germline sequence

G-score: similar to the H-score; attempts to classify which germline framework sequence the input antibody was originally derived from.

The databases for the H-score and G-score were composed of a small set of ~3,500 antibody sequences

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