High-throughput Gene Editing Vectors | Synbio Standard CRISPR Plasmid

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High-throughput Gene Editing Vectors

Our high-throughput gene editing vectors provide a robust vector skeleton that is essential for sgRNA library construction and delivery. These libraries can then be transfected into target cells through a range of methods, including viral infection and electroporation, making it easier than ever to perform large-scale gene editing experiments.

We use the latest techniques and technologies to ensure that our vectors are of the highest quality, offering unparalleled performance and efficiency in high-throughput gene editing applications. Unlike some other methods of gene editing, which can be time-consuming and labor-intensive, our vectors are optimized for speed and efficiency.

Highlights

Wide Species Coverage

Including plants, animals, and microorganisms
Variety of Editing Functions

Editing functions include activation, knockout, and inhibition
Personalized Customization

Provided alongside rich vectors resources

Technical Service

All Mammal Plant E.coli Yeast

Mammal

CRISPR Vector Name	Catalog	Plasmid Details	Vector Host	Screening Marker
CRISPR KO Vector (All-in-oneSystem)	STCRI24401	U6:sgRNA-EF-1a:Cas9-P2A-Puro	Mammals, Lentivirus	Amp/Puro
CRISPR KO Vector (All-in-one System)	STCRI24402	U6 :sgRNA-EF-1a:Cas9-P2A-EGFP	Mammals, Lentivirus	Amp/EGFP
CRISPR KO Vector (All-in-one System)	STCRI24403	U6:sgRNA-EF1a:Cas9-P2A-mCherry-Puro	Mammals, Lentivirus	Amp/Puro/mCherry
Cas9 Vector (Double-Vector System 1)	STCRI24404	EF-1a:Cas9-P2A-Blast	Mammals, Lentivirus	Amp/Blast
Cas9 Vector (Double-Vector System 1, Inducible expression Vector)	STCRI24405	Tight TRE promoter:Cas9-hPGK promoter:Blast	Mammals, Lentivirus	Amp/Blast
sgRNA Vector (Double-Vector System 2)	STCRI24406	U6:sgRNA-EF-1a:Puro	Mammals, Lentivirus	Amp/Puro
sgRNA Vector (Double-Vector System 2)	STCRI24407	EF1a:DsRed-Monomer-U6:sgRNA	Mammals, Lentivirus	Amp/Puro/DsRed
sgRNA Vector (Double-Vector System 2)	STCRI24408	U6:sgRNA-EF-1a:Puro-P2A-EGFP	Mammals, Lentivirus	Amp/Puro/EGFP
sgRNA Vector (Double-Vector System 2, Single cell screening)	STCRI24409	U6:sgRNA-EF-1a:Puro	Mammals, Lentivirus	Amp/Puro
CRISPR KO Vector (All-in-one System)	STCRI24410	CMV:SaCas9-U6:Sa-gRNA scaffold	Mammals, AAV	Amp
CRISPR KO Vector (All-in-one System)	STCRI24411	CMV:SaCas9-T2A-mCherry-U6:Sa-gRNA scaffold	Mammals, AAV	Amp/mCherry
Dual-sgRNA CRISPR KO Vector (All-in-one System)	STCRI24412	U6:sgRNA-U6:sgRNA-CBh:Cas9	Mammals	Amp
CRISPR KO Vector (All-in-one System)	STCRI24413	chicken β-actin promoter:Cas9-2A-Puro-U6:sgRNA	Mammals	Amp/Puro
Cas9 Vector	STCRI24414	chicken β-actin promoter:Cas9-2A-EGFP	Mammals	Amp/GFP
sgRNA Vector	STCRI24415	U6:sgRNA-PGK:Puro	Mammals	Amp/Puro/Fluc
CRISPRa Vector (Double-Vector System 1)	STCRI24416	EF-1a:dCas9-VP64-RelA(p65)AD-Rta AD-T2A-EGFP	Mammals, Lentivirus	Amp/EGFP
CRISPRa Vector (Three-Vector System, SAM System 1)	STCRI24417	EF-1a:dCas9-VP64-T2A-Blast	Mammals, Lentivirus	Amp/Blast
CRISPRa Vector (Three-Vector System, SAM System 1)	STCRI24418	EF-1a:dCas9-VP64-T2A-EGFP	Mammals, Lentivirus	Amp/EGFP
CRISPRa Vector (Three-Vector System, SAM System 2)	STCRI24419	EF-1a:MS2-N55K-p65-HSF1-T2A-HygR	Mammals, Lentivirus	Amp/HygR
CRISPRa Vector (Three-Vector System, SAM System 3)	STCRI24420	U6:sgRNA-MS2 stem loop-PGK:puro-T2A-BFP	Mammals, Lentivirus	Amp/Puro/BFP
CRISPRi Vector (All-in-one System)	STCRI24421	U6:sgRNA-hUbC promoter:dCas9-KRAB-T2A-Puro	Mammals, Lentivirus	Amp/Puro

Plant
CRISPR Vector Name	Catalog	Plasmid Details	Vector Host	Screening Marker
CRISPR KO Vector (All-in-one System)	STCRI24422	35s:Cas9-AtU6-26:sgRNA	Arabidopsis Thaliana	Kan/Hygro
CRISPR KO Vector (All-in-one System)	STCRI24423	35s:Cas9-AtU6-26:sgRNA	Arabidopsis Thaliana	Kan and Spec/Hygro
CRISPR KO Vector (All-in-one System)	STCRI24424	rice snoRNA U3 promoter :sgRNA- rice Ubi:Cas9	Rice	Kan/Hygro

E.coli
CRISPR Vector Name	Catalog	Plasmid Details	Vector Host	Screening Marker
CRISPRi Vector (All-in-one System)	STCRI24425	T5-lac operator:dCas9-J23119:sgRNA	E.coli	SmR
sgRNA Vector (Double-Vector System)	STCRI24426	J23119 (SpeI) :sgRNA	E.coli	Amp
sgRNA Vector (Double-Vector System)	STCRI24427	J23119 (SpeI) :sgRNA	E.coli	Spec
Cas9 Vector (Double-Vector System)	STCRI24428	tetR/tetA:SpCas9	E.coli	Amp

Yeast
CRISPR Vector Name	Catalog	Plasmid Details	Vector Host	Screening Marker
CRISPRa Vector (All-in-one System)	STCRI24429	EC2_10_dCas9_VPR_HC_sgRNA	Yeast	Kan/Neomycin (select with G418)
CRISPRi Vector (All-in-one System)	STCRI24430	EC2_3_dCas9_Mxi_sgRNA	Yeast	Kan/Neomycin (select with G418)

FAQs

How do high-throughput editing vectors support gene editing across different species?

High-throughput editing vectors are designed with broad species compatibility and features a specially optimized vector backbone that enables gene editing in a diverse range of species, including animals, plants, and microorganisms.

Apart from gene knockout, what other editing capabilities do high-throughput editing vectors possess?

High-throughput editing vectors offer more than just gene knockout capabilities; they also possess versatile editing capabilities such as gene activation and gene repression.

How is the quality and editing efficiency of high-throughput editing vectors ensured?

We recommend and customize the optimal vector based on the specific species and target sequence of interest. Furthermore, we adhere to rigorous quality control procedures, encompassing assessments of vectors' purity, concentration, structural integrity, and validation of editing efficiency.

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