*The delivery time of this step may vary due to the length and/or difficulties of the sequence(s). Please contact our project manager for a quote.

What is the difference between mammalian and bacterial expression?

Bacterial expression is fast and inexpensive, but often lacks proper protein folding and post-translational modifications. In contrast, mammalian expression provides more accurate protein folding and complex modifications like glycosylation, making it better for producing proteins with native-like structures and functions, especially for therapeutic proteins.

What Are the Different Methods for Delivering Genes Into Mammalian Cells for Protein Expression?

Introducing genes into mammalian cells is a critical step in protein expression research. Below are the most commonly used methods for this process:

♦ Transfection: A popular method for many cell types, transfection uses chemical reagents to introduce plasmid DNA into cells. It's especially effective for dividing cells, offering a simple and efficient way to deliver the gene of interest.

♦ Electroporation: Ideal for cell types that are hard to transfect or nondividing, electroporation uses an electrical field to create temporary pores in the cell membrane, allowing DNA to enter. While it requires specialized equipment, it provides high efficiency for specific cell types./li>

♦ Viral Transduction: Viral transduction leverages recombinant viruses to introduce genes into cells. This method is suitable for both dividing and nondividing cells and often involves engineered viral systems such as lentiviruses or adenoviruses. These viruses are modified to deliver the gene of interest while minimizing potential harm to the target cells.