Quality problems: low purity and sequence errors; these may lead to inaccurate sequencing results.
Design problems: If the adapter’s primers are not well designed, it may lead to poor binding with DNA samples or sequencing platforms, affecting sequencing efficiency.
Operational problems: improper handling of the adapter’s primers when preparing libraries or performing sequencing may lead to problems such as inaccurate concentration, uneven mixing, and so on.
Adapters are primarily used in two major processes: NGS library construction, which requires end repair of fragmented DNA, and ligation of sequence-specific adapters.
It is recommended to store adapters at -20℃.