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sgRNA Customized Design
No Species Limitations
Quote
Home > Gene Editing > sgRNA Customized Design
The General Introduction of sgRNA Customized Design

Synbio Technologies provides single sequence sgRNA design and whole-genome sgRNA library design, downstream verification, and stable cell line construction services. We offer a one-stop solution for CRISPR projects to achieve high genome editing efficiency.

Highlights
  • Well-designed Sequences
  • More Than 20 Designed Species
  • Customized sgRNAs
  • Single sgRNA, Multiple sgRNA and sgRNA Library Design Services
Service Details
Service Program Service Details TAT
sgRNA design The gRNA sequences are designed for specific gene classifications, with 3-6 sgRNAs designed for each gene 1-2 weeks
QUOTE
FAQs
When designing sgRNAs, how is the contamination of primer binding sites avoided?

    A unique primer binding site can be inserted into the screening library plasmid.

What online tools are available to help design sgRNAs?
What factors need to be considered for sgRNA design in mammalian cell studies?

    1. The GC content must be between 40% and 80% to ensure stable binding between the sgRNA and the target DNA.

    2. Removal of polyA sites, which disrupt viral packaging if viruses are used for delivery.

    3. Ensure that sgRNA designs have fewer secondary structures, such as hairpin structures and polymerase termination sequences; these structures can affect cloning efficiency and guide transcription.

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