The General Introduction of Standard sgRNA Library Cell Pool
The sgRNA plasmid library is introduced into target cells using methods like lentiviral infection. The cells are then screened for resistance, and the surviving population is known as the sgRNA library cell pool. This cell pool is a crucial tool for high-throughput genome editing at the cellular level. It supports basic research, such as studying gene and protein functions and signaling pathways, as well as medical applications like identifying therapeutic targets (e.g., for cancer and tumors), screening antibody targets, and conducting drug development and toxicity analysis. Synbio Technologies offers a one-stop service that covers everything from designing the sgRNA library sequences to constructing and screening the library cell pool. This integrated approach ensures optimal solutions and faster service delivery.
Highlights
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- Up to 90% Coverage
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- Supports the Construction of Any Cancer Cell Line as Target Cells
Service Details
• sgRNA library cell pool
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CRISPR sgRNA KO library cell pool with coverage >90%
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Service Programs
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Catalog
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Price
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Deliverables
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CRISPR sgRNA KO Library/HepG2
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SRKO240101
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Quote
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• Freezing cell （5*107）
• NGS resport
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CRISPR sgRNA KO Library/Hela
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SRKO240102
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CRISPR sgRNA KO Library/293T
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SRKO240103
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• Cas9 cell lines
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Cas9 stabilized cell lines used to create chassis cells for constructing sgRNA library cell pools in customer dual vector systems. Cell lines can be stably maintained as monoclonal cells for over a month.
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Service Programs
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Catalog
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Price
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Deliverables
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Cas9-Blast /293T
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SRCas240201
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Quote
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• Freezing cell（1*106）
• Instruction manual
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Cas9-Blast /HepG2
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SRCas240202
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Cas9-Blast /Hela
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SRCas240203
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• sgRNA library cell pool customization service
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The service program can be selected according to customer needs
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Service Programs
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Servicedetails
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TAT
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Deliverables
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sgRNA library cell pool construction(optional)
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Resistance screening detection
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6-9 weeks
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• Freezing cell
• NGS report
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Lentiviral load and cell count evaluation
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Resistance screening
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NGS detection
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Target drug screening(optional)
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IC50 testing
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5-7
weeks
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• NGS report
• Customized data analysis
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Drug screening
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NGS detection
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NGS detection
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Quote
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2-3
weeks
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NGS report
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FAQs
After completion, all sgRNA library cell pool constructs are tested with NGS to ensure they provide complete coverage and maintain high product quality.
The sgRNA coefficient in the sgRNA library cell pool service is 300-500x.
The customer will need to provide the name of the medication, information about your medication's potency test, and ensure that the medication has been sterilized.
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