Antisense Oligonucleotides|Synbio Technologies

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The General Introduction of Antisense Oligonucleotides

Synbio Technology offers antisense oligonucleotides (ASOs) that are specifically designed to target complementary RNA and prevent its translation into proteins. Our antisense oligos are ableto recognize specific mRNA sequences, thus blocking the translation of corresponding proteins, ultimately leading to the inhibition of gene expression.

Our synthesis process is optimized to produce high-quality antisense oligonucleotides that are engineered to provide maximum binding affinity and stability. By using state-of-the-art methods and cutting-edge technologies, such as HPLC purification and electrospray ionization mass spectrometry (ESI-MS), we ensure the highest levels of purity and accuracy. We are able to design and synthesize custom oligos for a variety of applications, including gene targeting, aptamer synthesis, and gene expression analysis.

Highlights

AI Design & Diverse Modifications

Uses AI-optimized design with various modifications (e.g., MOE, 2'-F, 2'-O-Me)
Excellent Quality Stability

Product purity >90%, batch-to-batch variation <0.01%
ISO 9001 and ISO 13485

Certified quality control ensures reliability
Manufacturing Capability

Microgram-to-gram-scale quantity
Delivery Speed

Guaranteed fast delivery times without compromising quality

Service Details

Service	Yield	Purification	Modifications	Deliverables	Turnaround Time
ASO Synthesis	R & D level: ug-mg	HPLC	PTO, 2′-F, 2′-OME, 2′-MOE, LNA, Cholesterol, GalNAc, etc.	● Dry powder delivery, according to the customer needs of the separate packing. ● COA files.	1 Week
ASO Synthesis	Manufacturing level: g	HPLC	PTO, 2′-F, 2′-OME, 2′-MOE, LNA, Cholesterol, GalNAc, etc.	● Dry powder delivery, according to the customer needs of the separate packing. ● COA files.	Inquire

* If you need other special modifications or pricing, please contact us for a quote at quote@synbio-tech.com.

QUOTE

Chemical Modification	Common Name	Class of Modification	Activates RNase H1	Binding Affinity Effects	Action
Phosphorothioate	PS	Backbone	Yes	Decreases	Binds non-specifically to proteins, exists as two stereoisomers
2'-O-Methyl	2'-OMe	Sugar	No	Increases	Natural RNA modification; decreases toxicity
2'-O-Methoxyethyl	2'-MOE	Sugar	No	Increases	Reduces protein binding, decreased toxicity
Locked nucleic acids	LNA	Sugar	No	Increases	High binding affinity at the cost of increased toxicity
5-Methylcytosine	5-MedC	Base	Yes	Increases	Natural RNA modification; prevents activation of TLR9 in CpG motifs
N-acetyl-galactosamine	GalNAc	Base	No	Decreases	Targets hepatocytes via receptor-mediated endocytosis
2'-Fluoro	2'-F	Sugar	No	Increases	Increases stability of RNA by preventing hydrolysis
Cholesterol	Cholesterol	Base (or non-nucleotide)	No	Increases	Improves cellular uptake and stability of oligonucleotides

QUOTE

FAQs

Can you guarantee sequence specificity?

Our tools allow us to guarantee sequence specificity with the utmost confidence.

Can you do ASO design? How many targets can be designed for one gene?

Synbio Technology can do ASO design. Depending on the customer's desire, a single gene can be designed with one or three targets.

How do you store the probes?

The probes should be stored at -20°C, away from light, and dispensed in brown tubes. It is recommended to use TE Buffer to dilute and store the probes. This will prevent repeated freezing and thawing, which reduces the service life of the probes.

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