The General Introduction of Lentivirus Packaging
Synbio Technologies has enhanced the safety of HIV-1-derived lentiviral vectors with a third-generation packaging system. These optimized lentiviruses deliver efficient, stable, and reproducible gene expression across all cell types, making them ideal for both dividing and non-dividing cells. We offer comprehensive virus packaging services, including overexpression, RNA interference, sgRNA, and library packaging.
Highlights
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- Long-lasting & Stable Expression of Target Genes
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- Enhanced Safety, Resulting in No Pathogenicity
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- Highly Customizable
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- Free Control Virus & Polybrene
Service Details
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Lentivirus Packaging
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Service Types
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Titer
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Volume
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Turnaround Time
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RNAi Lentivirus
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1*108 TU/ml or1*109 TU/ml
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1ml
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20-25 Business Days
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sgRNA Lentivirus
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1*108 TU/ml or1*109 TU/ml
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Overexpression Lentivirus
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1*108 TU/ml or1*109 TU/ml
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Library Series Lentivirus
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1*108 TU/ml or1*109 TU/ml
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Deliverables
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Free polybrene
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Free control virus
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Virus liquid, ≥108 TU/mL, 200 μL/tube
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QC report
FAQs
Human embryonic kidney cells (HEK293T cells) are commonly used due to their high transfection efficiency and ability to produce high titers of lentivirus. Other cell lines such as 293FT or 293H are also used.
For short-term storage, lentiviral particles can be kept at 4°C for up to a week. For long-term storage, aliquot and freeze at -80°C. Avoid repeated freeze-thaw cycles.
Second-generation lentiviral packaging systems use three plasmids, with some viral genes still present. This raises safety concerns due to potential recombination. In contrast, third-generation systems use four plasmids, separating the genes for structural and regulatory proteins into different plasmids. This enhances safety and minimizes the risk of replication-competent viruses. The separation makes third-generation systems safer for research and clinical use while maintaining high efficiency.
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