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The General Introduction of Diagnostic Probes & Oligos

Molecular diagnosis involves using nucleic acids or proteins as biomarkers to help diagnose and treat diseases more effectively.
Synbio Technologies offers a variety of diagnostic materials such as FISH probes, qPCR probes, SNP probes, chemical modification groups, fluorescence, and quenchers. We also provide products focused for use in molecular hybridization and PCR such as TaqMan/MGB qPCR probes, Molecular Beacons, Scorpions Probes, LNA Probes, Dual Quencher Probes, RAA/RPA Probes, etc. No matter how complex the synthetic sequences are, we always meet the requests of our customers.

Highlights

High Purity Guarantee

Probes have high purity and low background noise thanks to refined purification technology
Quality Assurance

Efficient removal of small molecule fluorescent dye residues; 100% LC-MS quality verification for each probe
Excellent Stability

ISO 9001 and ISO 13485 quality control management systems ensure complete control, traceability, and batch-to-batch consistency during the entire process
Highly Customized

Various synthetic specifications from nmol to umol, HPLC, PAGE, and other purification methods with high standards and purity are provided

Service Details

Synbio Technologies provides high quality nucleic acid probes with batch-to-batch consistency for qPCR/SNP detection. Our probes can also be labeled at the 5′ and 3′ ends of a designated position according to the specific needs of our customers. Depending on your project, we can modify these probes in order to improve the efficiency, accuracy, and diversity of qPCR assays.

Modifications		Purification
TAMRA	5’FAM-3’TAMRA, 5’HEX-3’TAMRA, 5’TET-3’TAMRA, 5’JOE-3’TAMRA	HPLC/PAGE
BHQ1	5’FAM-3’BHQ1, 5’HEX-3’BHQ1, 5’VIC-3′BHQ1, 5’NED-3′BHQ1, 5’TAMRA-3′BHQ1, 5’JOE-3’BHQ1
BHQ2	5’Cy5-3’BHQ2, 5’Cy3-3’BHQ2, 5’ROX-3’BHQ2
MGB	5’NED-3′ MGB, 5’TAMRA-3′ MGB, 5’ROX-3’MGB, 5’JOE-3’TAMRA, 5’Cy5-3’MGB, 5’Cy3-3’MGB, 5’VIC-3′MGB, 5’HEX-3’MGB, 5’FAM-3’MGB
DABCYL	5’FAM-3′ DABCYL, 5’HEX-3′DABCYL, 5’JOE-3′DABCYL, 5’ROX-3′DABCYL, 5’TAMRA-3′DABCYL, 5’CY5-3′DABCYL

* The above are our suggested purification methods. If you need other purification methods, please contact us for a quote at quote@synbio-tech.com.

* The estimated turnaround time for research use is 3-5 days and 1-2 weeks for industrial use.

Service Standards for Research or Industrial Use

Application Level	Research Application	Industrial Application
Synthetic Specification	nmol	umol
Turnaround Time	3-5 days	1-2 weeks
Price	Start from $125	Start from $185
Standard Delivery Project	Samples, COA file, MS or HPLC report.	Sample, COA file, MS or HPLC report, Fluorescence full wavelength scan, etc.
Purification	RP-HPLC/PAGE, etc.	RP-HPLC/IE-HPLC/Dual HPLC, etc.
QC	RP-HPLC detection ≥90%, LC-MS detection molecular weight deviation <0.1%, Full wavelength scanning (optional)	RP-HPLC detection ≥95%, IE-HPLC detection ≥95%, LC-MS detection molecular weight deviation <0.1%, full wavelength scanning (optional), others
Cross-Contamination	Conventional platform anti-pollution process	Special anti-pollution process
Delivery Form	Dry powder	Dry powder

Synbio Technologies offers standard oligos, fluorescent probes, special modifiers, skeleton modifiers, oligo pools, and other service lines to provide customers with a variety of customized FISH probe raw materials.

1.Multiple Fluorescence Types

Please select the appropriate fluorescence combination according to the fluorescence spectrum. The following are the commonly used fluorescence. If you need other fluorescence, please contact us for a quote at quote@synbio-tech.com.

FAM	Texas Red	AMCA
HEX	ROX	ATTO/Alexa488
VIC	Cy3	ATTO/Alexa 532
NED	Cy5	ATTO/Alexa 647

2.Duplex Stabilization

Several special base and skeleton modifications can avoid nucleic acid degradation and improve binding stability. Synbio Technologies can provide the following modifications.

Modifications	Improve the Tm Value	Prevent Nuclease Degradation
Phosphorothioate		√
2’-OMethyl	√	√
2’-Fluoro	√	√
LNA	√	√
5-Methyl-C	√

3.Syno Vision Fluorescence in Situ Hybridization Probe

Synbio Technologies utilizes the industry-leading high-throughput oligo pool synthesis platform to design and produce target-specific FISH probes. The probes produced by this platform have stronger specificity, an easier binding to target genes, and stronger fluorescence and stability.

Provided by Customers	Turnaround Time	Deliverable
● The physical location of the target area ● The target sequence ● Designed probe sequence	4-8 Weeks	Fluorescently labeled DNA probe solution

QUOTE

FAQs

What are the types of nucleic acid probes？

Depending on the source and nature, they are categorized as either genomic probes, cDNA probes, RNA probes or oligonucleotide probes.

How do you store the probes?

The probes should be stored at -20°C, away from light, and dispensed in brown tubes. It is recommended to use TE Buffer to dilute and store the probes. This will prevent repeated freezing and thawing, which reduces the service life of the probes.

What is the difference between qPCR probe and FISH probe?

qPCR is commonly used in the detection of infectious disease pathogens, the research of drug resistance genes, the evaluation of drug efficacy, and the diagnosis of genetic diseases.

FISH probeare use to detect gene amplifications, deletions and rearrangements for chromosomal localization of known genes or sequences, uncloned genes or genetic markers, and chromosomal aberrations.

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