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Gene Shuttle Vector

Synbio Technologies is excited to introduce a new service: Gene Shuttle Vector. Our modified shuttle vector enables protein expression detection without requiring transformation operations.

Compared to existing expression vector in the market,our shuttle expression vectors offer enhanced efficiency and reliability. These vectors streamline experimental procedures, significantly reducing complexity. Additionally, their high-efficiency dual-system compatibility enhances expression capabilities. When used in combination with our ProXpress rapid protein detection kit, you can preview the expression of synthesized plasmids before receiving them, speeding up the progress of scientific research!

Highlights

Highly efficient and stable protein expression in eukaryotic and prokaryotic systems.
No need to be transformed into BL21 ( DE3 ) cells, shortening the verification cycle.
Instant protein expression detection with ProXpress.
Customized vector transformations available to meet specific research needs.

Service Details

Service Types	Gene Length	Price	Turnaround Time (Business Days)
GeneShuttleVector	<251 bp	$50	5-10
	251-1,500 bp	$0.20/bp	5-10
	1,501-3,000 bp	$0.21/bp	10-15
	3,001-4,500 bp	Quote	15-20
	4,501-6,000 bp	Quote	20-25
	6,001-8,000 bp	Quote	Quote
	>8,000 bp	Quote	Quote

* Turnaround time applies to gene synthesis of non-complex sequences. For gene synthesis of complex sequences, please consult: quote@synbio-tech.com.

* Optional protein validation with ProXpress for $35 each.

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Workflow

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Deliverables

2~5 µg lyophilized plasmid DNA

Sequencing chromatogram

Certificate of analysis (COA)

ProXpress validation reports

Case Study

1. Background：

The shuttle vector expressing green fluorescent protein (GFP) wastransferred into prokaryotic cells ( Mach1-T1 ).

The results show that :

The supernatant of the bacterial lysate was observed under ultraviolet (UV) light and green fluorescence was detected.

Using the Flag-tag protein rapid detection card,protein expression was confirmed10 minutes later,showing high levels of proteinexpression.

2. Background：

Aconstructed shuttle expression vector was transfected into HEK293 cells and compared witha conventional expression vector.

The results show that :

Comparedto the conventional vector, the modified shuttle expression vectorshowed significantlyhigherproteinexpression in HEK293 cells.

FAQs

What are the possible reasons for failed gene cloning when inserting a target gene into a shuttle vector?

Possible causes include:

Unreasonable primer design
Poor template quality
Incorrect PCR program settings.

Compared to conventional vectors, what advantages do shuttle vectors offer?

Dual system compatibility: Shuttle vectors contain two or more replicons from different genetic backgrounds,enabling replication in two or more different organisms.
Selective markers: Shuttle vectors usually contain selective markers, such as antibiotic resistance genes, allowing for easy screening of clones containing the target gene via antibiotic selection.
Higher Efficiency: Shuttle vectors are typically more efficient when introduced into the target biological system.

What are the application areas of shuttle vectors?

Shuttle vectors are used in various fields, including:

Gene function research
Protein interaction analysis
Drug target screening
Vaccine development

The efficiency of shuttle vectors makes them suitable for various application, ranging from academic research to drug development,supporting scientists and researchers in their pursuit of research and innovation.

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