PCR Cloning & Subcloning
PCR cloning and
subcloning, as commonly used recombination techniques, are widely used in
genetic engineering, agricultural production, biopharmaceuticals, etc. PCR
cloning is a rapid method of cloning genes, which does not depend on the
enzymatic sites of the vector, and can directly clone the target gene fragments
into the target sites of any vector. Subcloning refers to
the use of existing enzymatic sites to transfer them from one vector to another.
Synbio Technologies, leveraging the integration of
DNA synthesis tools and cloning technologies, offers comprehensive PCR cloning and subcloning services. Our services are capable of cloning the target gene into any desired location of the vector, fulfilling your specific design requirements. For subcloning the same gene fragment into different vectors, we also provide efficient services to ensure the smooth progress of your downstream experiments.
Highlights
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- Fast Turnaround Time
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- 100% Sequence Accuracy
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- Independent of Vector Restriction Sites
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- Professional Technical Team
Service Details
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Service Type
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Fragment Length
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Turnaround Time
(Business Days)
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Price
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PCR Cloning & Subcloning
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<2 kb
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5-10
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Starting from $75
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2-3 kb
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5-10
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>3 kb
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Quote
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* The turnaround time applies to non-complex sequences. Please contact quote@synbio-tech.com to quote for the complex sequences.
* Note
DNA Sequence
If the sequence has not been verified, we will provide Sanger sequencing verification. (Additional fees apply)
Specific Vectors
1. A Full-length sequence is required.
2. If there is no sequence information, we will provide Sanger sequencing verification. (Additional fees apply)
Rare RE or Special Molecular Biological Reagents
1. Customers will provide these reagents.
2. Synbio Technologies will purchase them for you (Additional fees apply) and return the excess after the delivery.
Workflow
Deliverables
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Lyophilized plasmid DNA (2~5 µg)
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Sequencing results in .ab1 format
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Certificate of Analysis (COA)
FAQs
The PCR subcloning service is a molecular biology service based on the Polymerase Chain Reaction (PCR) technology. It is utilized to amplify specific DNA fragments from complex DNA samples and subsequently clone them into vectors, facilitating further genetic engineering or research.
PCR cloning services have extensive applications in the field of molecular biology, including gene cloning, gene expression analysis, gene mutation detection, genetic disease diagnosis, and more. Leveraging the PCR cloning technology, researchers can efficiently amplify and clone target DNA fragments, providing robust support for genetic research and applications.
• Optimize PCR Conditions: Ensure high purity and concentration of the target DNA fragments obtained through PCR amplification.
• Select an Appropriate Vector: Choose the suitable vector type and cloning sites based on the experimental requirements.
• Strictly Control Experimental Conditions: Rigorously manage factors such as temperature and time during the ligation and transformation processes.
• Conduct Multiple Rounds of Screening and Verification: Obtain positive clones through multiple rounds of screening and identification, followed by sequencing validation.
Resources
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