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PCR Cloning & Subcloning

PCR cloning and subcloning are two primary methods for amplifying DNA sequences. PCR cloning mimics the natural replication process of DNA, enabling the amplification and purification of DNA fragments in vitro. Subcloning involves selecting a fragment of DNA from cloned products and inserting it into a new vector. These techniques are widely applied in gene cloning, gene expression analysis, and other fields.

Synbio Technologies, leveraging the integration of DNA synthesis tools and cloning technologies, offers comprehensive PCR cloning and subcloning services. Our services are capable of cloning the target gene into any desired location of the vector, fulfilling your specific design requirements. For subcloning the same gene fragment into different vectors, we also provide efficient services to ensure the smooth progress of your downstream experiments.

Highlights

Fast Turnaround Time
100% Sequence Accuracy
Independent of Vector Restriction Sites
Professional Technical Team

Service Details

Service Type	Fragment Length	Turnaround Time (Business Days)	Price
PCR Cloning & Subcloning	<2 kb	5-10	Starting from $75
	2-3 kb	5-10
	>3 kb	Quote

* The turnaround time applies to non-complex sequences. Please contact quote@synbio-tech.com to quote for the complex sequences.

* Note

DNA Sequence

If the sequence has not been verified, we will provide Sanger sequencing verification. (Additional fees apply)

Specific Vectors

1. A Full-length sequence is required.

2. If there is no sequence information, we will provide Sanger sequencing verification. (Additional fees apply)

Rare RE or Special Molecular Biological Reagents

1. Customers will provide these reagents.

2. Synbio Technologies will purchase them for you (Additional fees apply) and return the excess after the delivery.

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Workflow

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Deliverables

Lyophilized plasmid DNA (2~5 µg)

Sequencing results in .ab1 format

Certificate of Analysis (COA)

FAQs

What is PCR subcloning service?

The PCR subcloning service is a molecular biology service based on the Polymerase Chain Reaction (PCR) technology. It is utilized to amplify specific DNA fragments from complex DNA samples and subsequently clone them into vectors, facilitating further genetic engineering or research.

What are the applications of PCR cloning service?

PCR cloning services have extensive applications in the field of molecular biology, including gene cloning, gene expression analysis, gene mutation detection, genetic disease diagnosis, and more. Leveraging the PCR cloning technology, researchers can efficiently amplify and clone target DNA fragments, providing robust support for genetic research and applications.

How do I improve the success rate of PCR subcloning?

• Optimize PCR Conditions: Ensure high purity and concentration of the target DNA fragments obtained through PCR amplification.

• Select an Appropriate Vector: Choose the suitable vector type and cloning sites based on the experimental requirements.

• Strictly Control Experimental Conditions: Rigorously manage factors such as temperature and time during the ligation and transformation processes.

• Conduct Multiple Rounds of Screening and Verification: Obtain positive clones through multiple rounds of screening and identification, followed by sequencing validation.

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