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Gene Synthesis

Gene synthesis refers to the technology of artificially synthesizing double-stranded DNA molecules in vitro, serving as one of the methods to obtain target genes. It is characterized by short synthesis cycle and is not limited by the source of the template. Gene synthesis is widely applied in various fields, including genetic engineering, synthetic biology, and biomedical research, enabling scientists to study, modify, and even create new genes and organisms.

Synbio Technologies is dedicated to providing customers with rapid, precise, and reliable customized gene synthesis solutions. With ten billion bases of synthesis experience and an advanced vector design platform, we expertly overcome complex gene synthesis challenges, including high/low GC content, repetitive sequences, hairpin structures, etc. By purchasing, we also offer free codon optimization, vector build, and storage. Our products are of the highest quality with competitive prices. We guarantee 100% accuracy in DNA synthesis and cloning to your preferred vector!

Highlights

Optional

Free codon optimization, vector build, and storage
AI-powered

Perfect solution to complex gene synthesis
Cost-effective

Lower prices and faster TAT
Accuracy

Deliver 100% accurate DNA sequences

Service Details

Service Types	Gene Length	Price	Turnaround Time (Business Days)
Standard Gene Synthesis	<251 bp	$50	5-10
	251-1,500 bp	$0.20/bp	5-10
	1,501-3,000 bp	$0.21/bp	10-15
	3,001-4,500 bp	Quote	15-20
	4,501-6,000 bp	Quote	20-25
	6,001-8,000 bp	Quote	Quote
	>8,000 bp	Quote	Quote

* Turnaround time applies to gene synthesis of non-complex sequences. For gene synthesis of complex sequences, please consult: quote@synbio-tech.com.

* Optional protein validation with ProXpress.

Default Vector Information

Vector Vame	Resistant	MCS	Vector Map and Sequence
pUC57-Amp	Amp	Yes	Download
pUC57-Kan	Kan	Yes	Download

* The genes will be cloned into our default pUC57-Amp or pUC57-Kan vectors with multiple cloning sites. We can also clone the genes into your supplied vectors, upon request.

How to Order

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How Gene Synthesis Ordering System Makes Everything Easier？View More

Intelligent Sequence Complexity Evaluation

Our new system uses advanced algorithms to automatically analyze sequence complexity, providing detailed information about your gene without the need for manual calculations.

Protein Sequence Optimization
With a built-in protein sequence optimization module, the power to significantly improve both the stability and expression efficiency of your gene sequence is in the palm of your hands.

Real-time Quotes

Simply input your gene sequence and our system will automatically calculate the synthesis cost based on length, complexity, etc., providing a valuable project budget reference.

Our new gene synthesis order system is designed to revolutionize the way you synthesize genes. With a user-friendly interface and streamlined quotation process, ordering custom genes has never been easier. Your access to better, smarter genes is finally here!

Deliverables

2~5 µg lyophilized plasmid DNA

Sequencing chromatogram

Certificate of analysis (COA)

Case Study

A case of complex gene synthesis and assembly for a 14kb fragment

Successfully synthesized a 14kb complex gene fragment and assembled the synthesized gene of interest onto pSynoYac0-Cm (approximately 10kb).

Difficulty Analysis of CI System

● The GC content in the target fragment fluctuates significantly, with some regions reaching as high as 100%.

● There are multiple repetitive sequences present within the target fragment.

GC Content Analysis of the Target Fragment

Repeat Sequence Analysis Chart of Target Fragment

FAQs

What are the factors that affect gene synthesis?

The factors that affect gene synthesis are primarily determined by sequence length, DNA structure, (including repetitive sequences, GC content, and other factors), and host cell stability.

What is frameshift mutation?

Frameshift mutation occurs when the amount of nucleotides in a gene increases or decreases by a number that is not a multiple of three. Since three nucleotides encode for one amino acid, this alteration causes a shift in the reading frame of the DNA sequence. This results in the production of a non-functional or altered protein that differs from the intended one.

What is codon bias?

Codon bias refers to the unequal usage frequency of synonymous codons (codons that encode for the same amino acid) among different organisms. Understanding codon bias is crucial for optimizing gene expression in specific hosts.

More FAQs>>

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