Nanobody Customization Services

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General Introduction of Nanobody Customization Services

Nanobodies, or single-domain antibodies, originate from the VHH region of camelid heavy-chain antibodies such as those in alpacas. At only about 15 kDa, roughly one-tenth the size of conventional antibodies, they feature a simple structure, strong stability, and high affinity, making them widely useful in tumor immunotherapy and cell-based therapies.

Synbio Technologies provides a complete workflow from antigen preparation and animal immunization to expression and purification. With expertise in antigen design, alpaca immunization, library construction, and high-throughput screening, we offer efficient and reliable nanobody customization to help turn your target concepts into practical research tools or potential therapeutic candidates.

Highlights

Traceable Source

Own alpaca breeding base with clear traceability; animals are natural and unimmunized.
Technical Expertise

Strict QC at every step, from immunization, library construction to screening, supported by extensive experience.
One-Stop Delivery

Simply provide your target or antigen, and we handle everything from immunization to sequencing verification and protein delivery.
Customized Solutions

Development strategies tailored to your specific applications, selecting the most suitable nanobody for your needs.

Service Details

Stage	Service Content	TAT	Deliverables
Stage 1: Antigen Preparation (Optional)	* Protein antigen purity and endotoxin identification (>5mg/protein antigen) * Protein antigen conjugation to carrier protein followed by immunization (Optional)	2-4 weeks	Protein Antigen Identification Report 200ug of antigen conjugated to carrier protein
Stage 2: Alpaca Immunization & PBMC Isolation	* 1 adult immunized alpaca, immunized with two protein antigens 5+ times, 0.5-1mg each time * PBMC Isolation	8-10 weeks	ELISA report for post-3rd immunization serum
Stage 3: Construction of VHH Phage Display Antibody Library	* RNA extraction and cDNA preparation * Amplification of VHH gene fragments, construction of nanobody immune library * Library quality control: Pick 96 single clones for sequencing, analyze sequence diversity and correctness	4-6 weeks	Intermediate products (e.g., RNA or cDNA); Provide library QC report, VHH library size of 10⁸–10⁹；Phage Display Nanobody Immune Library (library phage particles 10ug)
Stage 4: Phage Display Panning & Clone Identification	3-5 rounds of panning using antigen pressure * Pick at least 288 clones for ELISA validation * Identify and evaluate positive clones * Sequencing of positive clones and analysis of sequencing results	4-6 weeks	Eluate from the last round of panning; ELISA screening report; Provide 3-5 unique sequences per protein antigen
Stage 5: Nanobody Expression, Production & Validation	* Clone 3-5 nanobody genes into expression vector * Recombinant antibody expression and SDS-PAGE validation	2-4 weeks	0.1-0.5mg purified nanobody (SDS-PAGE >90%, endotoxin level < 1EU/mg) and corresponding strain; Nanobody ELISA test report

Note: The requirements of antigen provided by customers: purity >85%, concentration > 1 mg/mL, PBS buffer.

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Workflow

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Case Study

Phage screening-phage display library construction and detection

Phage Screening - Monoclonal Screening

FAQs

What are the core advantages of Nanobodies compared to traditional antibodies?

Nanobodies derive key benefits from their unique single-domain structure:

Superior Tissue Penetration: Their small size (~15 kDa) enables access to difficult epitopes within solid tumors or behind the blood-brain barrier.

High Stability & Solubility: They exhibit exceptional thermal, pH, and chemical stability for room-temperature storage, along with high solubility and low aggregation.

Ease of Engineering: As single genetic units, they can be readily designed into multispecific formats, CAR-T cells, or drug/imaging conjugates, offering great application flexibility.

What are the main application areas for Nanobodies?

Leveraging their unique properties, Nanobodies hold significant promise across multiple advanced fields:

Therapeutics: Function as targeted drugs (e.g., immune checkpoint inhibitors), components of delivery systems (e.g., ADCs), and potent viral neutralization tools (e.g., against SARS-CoV-2, influenza).

Diagnostics: Serve as critical reagents in in vitro assays (e.g., ELISA, lateral flow) for enhanced stability and sensitivity, and as radiolabeled probes for in vivo imaging (e.g., PET/CT) enabling precise tumor detection.

Research Tools: Utilize in super-resolution microscopy for protein localization and act as "crystallization chaperones" to facilitate structural biology studies.

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