A: In vitro transcribed RNA using nucleotide chemical modification strategy can reduce immunogenicity without affecting its translation properties. Conventional RNA modification replaces natural adenosine with N1-methyladenosine (m1A) or N6-methyladenosine (m6A). Replace natural cytidine with 5-methylcytidine (m5C); Replace natural uridine with 5-methoxyuridine(5moU),N1-methyl-pseudouridine(m1ψ),pseudouridine(ψ),etc.

A: At present, we can successfully deliver RNA ranging from 100bp to 8500bp. Among them, transcription RNA above 5000bp in vitro may have complex secondary structures and low integrity. The sequences provided by the customer require additional evaluation and sequence optimization.

A：Ready to ship, it can be expressed in mammalian cells.

A: Sp6 can be used, but the expression effect cannot be guaranteed. We default to using the T7 promoter.

A: Yes，we have firefly luciferase mRNA.

A: We have the following purification methods to choose from based on customer needs: silica gel membrane centrifugal column purification; Purification of lithium chloride; Oligo (dT) purification; Reverse chromatography purification; HPLC-SEC purification.

A: We offer two shipping options: liquid shipping (sterile enzyme free water) and dry powder shipping (non freeze-dried).