At present, most of the products on the market use human genome sgRNA libraries. The screening results obtained from genome sgRNA libraries are more broad-spectrum, but the experimental engineering is large. And Syno human pathway library has the following characteristics:
1、The total number of genes is small, and the storage capacity of the sgRNA library is small, which reduces the amount and production time of experimental engineering.
2、The use of Syno human derived sgRNA libraries has strong specificity, usually through target screening within a known gene range.
3、Multiple pathways can be selected for simultaneous research.
Therefore, before constructing using a Syno human source sgRNA library, it is important to understand the research objectives and pathway genes, and select a single pathway or a combination of multiple pathway sgRNA libraries according to the needs.