A: In addition to conventional gene synthesis, we also provide gene synthesis and molecular biology related services such as long fragment gene synthesis, PCR cloning, subcloning, site-directed mutagenesis, plasmid construction, vector modification, etc. The cycle and charge vary according to the length of synthesis and the difficulty of the sequence.

A: The pUC57 vector containing Amp (ampicillin) is provided for free by default; meanwhile, there are more than 100 optional vectors in GenScript's vector library, which are available for you to use free of charge. You can also bring your own vector, but you only need to provide the vector sequence and vector sample. It is recommended to mail your own vector in advance.

A: Yes, it is available for free. The same amino acid corresponds to codons that show different degrees of preference in different hosts. Codon optimization refers to using preferred codons and avoiding rare codons to optimize the expression of the sequence in the specified host. The main optimization parameters include: codon preference, GC content, avoidance of specific restriction enzyme sites, poly structures, sequence repeats, etc.

A: For genes used for protein expression, it is necessary in most cases. For example, when eukaryotic genes need to be expressed in prokaryotes. Since the codon preferences of eukaryotes and prokaryotes are quite different, codon optimization of genes will significantly improve the expression efficiency.

 (1) The default shipped sample is 2-5 µg of high-purity lyophilized plasmid DNA containing the target fragment;

 (2) The shipping documents mainly include the following contents: the standard sequence of the target gene, the full-length sequence of the plasmid containing the target gene, the original file of the sequencing results, the sequence alignment file, the plasmid map, and the shipped sample report (COA).

A: Our project management team will send progress emails every week. If you do not receive the email or are in a hurry, you can email service@synbio-tech.com or contact the corresponding sales representative for inquiry.

A: Throughout the entire gene synthesis project, we adopt strict and effective quality control measures to ensure the accuracy of the synthesized gene. Firstly, before the project starts, the project manager will analyze your sequence, write the order according to your requirements, and then ask you to verify whether the sequence information and requirements in the order are correct. For the shipped plasmid, we will ensure 100% correctness through Sanger sequencing, and at the same time perform restriction enzyme digestion verification on the plasmid to ensure that the bands fully meet the expectations. The QC department will carefully review the shipped samples, and the samples will be shipped only after passing the review. Finally, the shipping documents of the order and the courier tracking number of the shipping list will be sent to your email address in the form of an email.

A: Plasmids can be stored for one year, while bacterial cultures will not be stored. If the customer requires storage for more than one year, it can be specified in the contract, and an additional fee will be charged to extend the storage time. The fee is $300. After one year, each time the customer uses them, a fee of $100 will be charged, with a maximum of one charge per year, and no fee will be charged if they are not used.

A: Yes, the pSmart-I plasmid.

A: This service belongs to the Trimer primer synthesis service. Specifically, the following need to be confirmed: 1. Is it to synthesize a library or just to do primer synthesis? 2. Specific sequence information and mutation site information, including the number of sites to be mutated, and whether the mutation is saturation mutation or has specified requirements on the proportion of mutated amino acids? 3. The full-length sequence and vector information need to be confirmed.

A: For gene synthesis, customers need to provide plasmids.

A: Yes, it is gene synthesis. We only need customers to provide the heavy chain and light chain sequences of the antibody.