Synbio Technologies requires the insert sequence, cloning vector information and cloning sites to generate a formal quotation for gene our gene synthesis services.

Material Instructions 

1. Keep the vial sealed until ready to use. 

2. Centrifuge at 6,000 x g for 1 minute at 4°C. Open the vial and add 20 μl of sterilized water to dissolve the DNA. 

3. Close the lid and vortex the vial for 1 minute. If necessary, heat the solution at 50°C for 15 minutes to dissolve the DNA. 

4. Transformation of the plasmid DNA can be performed directly after completion of the steps above. Verify the plasmid sequence after each subcloning and transformation step.

A gene that has been cloned into a vector, both commercial and custom, have been verified with Sanger Sequencing and confirmed to have 100% sequence identity to the expected sequence. 

A gene delivered as a linear PCR product represents a mixture of correct and incorrect sequences due to errors during polymerase chain reaction. Synbio Technologies utilizes specialized techniques to minimize these

Synbio Technologies offers various other services that can be bundled with gene synthesis such as custom cloning, plasmid preparation, site-directed mutagenesis and protein expression. Our dedicated project management team will analyze your request and apply the most effective service to fit your needs.

Synbio Technologies has a large library of commercial vectors that can be used for cloning for a range of expression systems.

Synbio Technologies' DNA synthesis lengths up to 200Kb.

After DNA synthesis is completed, Synbio Technologies usually delivers DNA synthesis products based on customer requirements and the specific nature of the project.

For most customers, Synbio Technologies will use a trusted delivery service to ship synthetic DNA products. This ensures that the product is delivered to the customer 's designated location quickly and safely. If DNA synthesis products need to be stored at a low temperature, we will use dry ice transport to ensure that the product remains stable during transport.

Synbio Technologies is dedicated to providing customers with rapid, precise, and reliable customized gene synthesis solutions. With ten billion bases of synthesis experience and an advanced vector design platform, we expertly overcome complex gene synthesis challenges, including high/low GC content, repetitive sequences, hairpin structures, etc. We guarantee 100% accuracy in DNA synthesis and cloning to your preferred vector!

• Biomedicine: DNA synthesis productsare used to develop gene drugs targeting genetic diseases or cancer, as well as DNA probes for gene diagnosis and treatment.

• Agriculture: DNA synthesis products aid in cultivating crops that are resistant to insects, drought, and have high yields, improving crop production and quality while reducing pesticide and fertilizer use.

• Industrial Production: DNA synthesis products optimize microbial metabolic pathways, enhancing the yield and quality of fermentation products.

• Basic and Scientific Research: DNA synthesis products facilitate the study of gene function and regulatory mechanisms, simulating signal transmission and regulation processes within organisms.

• Environmental Protection: DNA synthesis products help cultivate microorganisms that degrade pollutants.