1. Trimer primer sequence; 

2. The types and proportions of amino acids in each primer; 

3. Codon table (optional)

Q: Can 1mg of CpG be divided into 100ug per tube?

A: The shipping form is by default 1mg per tube, without division. After receiving the samples, customers can dissolve and divide them into frozen aliquots, using one tube at a time.

A: ddH2O and physiological saline

(1) Fluorescent modification, which can carry fluorescent groups (such as FAM, Cy3, FITC, etc.)

(2) Functional modifications, such as NH2 modification, THS modification, N3 modification, DBCO modification, etc., mainly for connection purposes, and GalNac mainly for liver targeting functions.

A: The CpG is shipped at room temperature, and the dry powder can be stored at room temperature for several months. After receiving the samples, customers are advised to store them long-term at -20℃. After dissolution, it is recommended to divide it into small aliquots according to the customer’s needs, and the unused ones can be stored at -20℃ to avoid repeated freezing and thawing cycles.

The following modifications are commonly used. The most commonly used types of modifications are five 2 '- MOE modifications before and after each end of the sequence, and other full thio modifications. The length is generally 20nt. We can help designing and synthesizing the sequence, with the design only ensuring sequence specificity.

A: Yes.

A: In addition to conventional gene synthesis, we also provide gene synthesis and molecular biology related services such as long fragment gene synthesis, PCR cloning, subcloning, site-directed mutagenesis, plasmid construction, vector modification, etc. The cycle and charge vary according to the length of synthesis and the difficulty of the sequence.

A: The pUC57 vector containing Amp (ampicillin) is provided for free by default; meanwhile, there are more than 100 optional vectors in GenScript's vector library, which are available for you to use free of charge. You can also bring your own vector, but you only need to provide the vector sequence and vector sample. It is recommended to mail your own vector in advance.

A: Yes, it is available for free. The same amino acid corresponds to codons that show different degrees of preference in different hosts. Codon optimization refers to using preferred codons and avoiding rare codons to optimize the expression of the sequence in the specified host. The main optimization parameters include: codon preference, GC content, avoidance of specific restriction enzyme sites, poly structures, sequence repeats, etc.

A: For genes used for protein expression, it is necessary in most cases. For example, when eukaryotic genes need to be expressed in prokaryotes. Since the codon preferences of eukaryotes and prokaryotes are quite different, codon optimization of genes will significantly improve the expression efficiency.

 (1) The default shipped sample is 2-5 µg of high-purity lyophilized plasmid DNA containing the target fragment;

 (2) The shipping documents mainly include the following contents: the standard sequence of the target gene, the full-length sequence of the plasmid containing the target gene, the original file of the sequencing results, the sequence alignment file, the plasmid map, and the shipped sample report (COA).