A: Conventionally, it is 5’-Chol, and partially 2’-Ome modification.

A: The quotation is based on the required amount of synthesis, the base length, and the type of modification.

A: siRNA is chemically synthesized RNA (without strain), with a "three guarantees one" policy. shRNA is gene synthesis (with strain), and it is divided into "three guarantees one" and "four guarantees one" types.

A: It is desalination/HPLC purification.

1. Ordinary negative control
The general negative control is an ordinary negative control that has no homology with the sequence of the target gene;
The negative control needs to ensure that it has very low homology with other genes in the target cells.

2. Fluorescently labeled negative control

The RNAi negative control has no homology with mammalian genes;

By labeling with fluorescence, the transfection status can be conveniently observed under a fluorescence microscope;

It can be used to optimize transfection conditions and evaluate transfection efficiency;

It has good pH tolerance and is more stable in living cells.

siRNA positive control

3. Positive controls can be used to confirm that the methods for transfection, RNA extraction, and gene expression detection in RNAi experiments are reliable.

Commonly used siRNA positive controls are as follows:

LaminA/C, GFP22, Luciferase GL2, MAPK1, Beta-Actin, Vimentin, P53, GAPDH, Cyclophilin B

A: Yes, plasmid synthesis can also have "three guarantees one" or "four guarantees one".

A: Quotations are made by category, which are ready-to-use sgRNA chemical synthesis, in vitro transcription, and only plasmid construction.

Yes, Synbio Technologies offers a wide variety of fluorophores to be included as modifications for the delivered RNA oligos.

Synbio Technologies offers the synthesis of siRNA, miRNA, sgRNA, short and long stranded RNA, and RNAi services.

Yes, Synbio Technologies offers in vitro RNA synthesis services. This allows us to deliver long RNA sequences, both single and double stranded, to our customers.

Synbio Technologies offers the synthesis scale and yield from small scale, 2 OD yield, up to and exceeding 100 OD. Our lab offers custom services to fit our customer’s yield requirements.

The turnaround time will vary depending on the type of RNA synthesis, overall length of the RNA and yield. The typical turnaround time starts at 5 – 7 business days.