(1) Dry products are very stable and can be stored at room temperature for several months without any issues. However, to ensure absolute stability, it is better to store them at -20℃.

(2) For dissolved DNA solutions, they can be stored at 4℃ for short-term use (1-2 weeks). For long-term storage, it is recommended to store them at -20℃. Avoid repeated freeze-thaw cycles. It is advisable to divide the liquid into multiple aliquots for storage. (When dissolving DNA, please note to use sterile, nuclease-free water or TE Buffer).

(3) Fluorescently labeled primers should be stored in the dark.

A: Quantification can be done using a UV spectrophotometer to measure the absorbance of the solution at a wavelength of 260nm. The absorbance of the solution should ideally be diluted to between 0.2 and 0.8 (too high or too low absorbance can result in significant errors). For the lyophilized DNA, dissolve it thoroughly in a certain volume of water by shaking, then take a portion of the solution and dilute it to 1ml. Measure the absorbance in a 1ml standard cuvette, which will give you the OD value for the measured volume, and from there, you can calculate the OD value of the mother solution.
Oligo products shipped by Synbio Technoligies come with quantification information on the shipping tube label and in the COA report, and suggested dilution volumes are provided for the convenience of customers' experiments. You can directly refer to the values in the COA to calculate parameters such as concentration.

A: Conventional primers under 150 nt; modified primers up to 140 nt; ssDNA up to 4K; RNA synthesis up to 110 nt. For gene synthesis services, the longest completed project is 24K, and we are currently constructing several 30K genes.

(1) Check if the oligo and template are complementary, and ensure that the 15bp at the 3' end of the oligo are completely matched with the template sequence;

(2) Check if the oligo itself has any hairpin structures or high GC, poly structures;

(3) Verify if the PCR reagents and PCR machine are functioning properly;

(4) Ensure that the PCR reaction conditions are appropriate;

(5) Check if the control amplifies normally.

A: This depends on the experimental purpose. For example, for a primer around 20 bases: for conventional PCR amplification, you can choose 5 nmol (approximately 1 OD), which is sufficient for 200 times 50 μl standard PCR reactions; for gene splicing or annealing followed by ligation, you can also choose 5 nmol (approximately 1 OD).

(1) For general PCR amplification: 60base PAGE plus/60base PAGE

(2) For diagnostic PCR amplification: 40base PAGE plus

(3) For DNA sequencing: around 20base PAGE plus

(4) For subcloning, point mutation, etc., choose PAGE plus, PAGE, HPLC based on experimental requirements

(5) For gene construction (full gene synthesis): choose PAGE plus, PAGE based on experimental requirements

(6) For antisense nucleic acids: choose PAGE-plus, PAGE based on experimental requirements

(7) For modified primers: choose PAGE, HPLC based on experimental requirements

Additionally, SIMA has the same color as HEX, and FITC has the same color as FAM.

A: FAM, VIC, biotin, CY5, TXR, etc..

A: Generally, a mass spectrometry report is provided for primers with modifications at both ends. Other services do not include a mass spectrometry report.

A: The minimum delivery quantity is 1OD. The middle can have any degenerate bases, including NNK.