Another strategy of RNA synthesis is by in vitro transcription, especially suitable for long RNA synthesis.
In vitro transcription takes linear DNA sequences as a template, using T7, T3, or SP6 RNA polymerase to synthesize RNA from the DNA sequence.
Synbio Technologies provides highly efficient and cost-effective in vitro transcription RNA synthesis services. You can provide us with the DNA templates, such as plasmid and PCR product, or we can help conduct the de novo gene synthesis of the target DNA sequences. In our design, we synthesize the T7 promoter before the target sequence, which is used to initiate the transcription reaction during the RNA synthesis process. For mRNA transcription, Synbio Technologies provides optional mRNA modifications, such as using mRNA capping enzymes from the vaccinia virus to add cap structures or using E. coli poly(A) polymerase to add poly(A) tails of the transcript RNA. Moreover, our RNA specialty service also includes sgRNA synthesis, you can use the synthesized sgRNA to transform the cell along with Cas9 proteins or Cas9 mRNA to perform downstream targeting.
The Synbio-Tech Difference
In Vitro Transcription Process
Templates for In Vitro Transcription
dsDNA templates often increase transcription yield of the RNA. Synbio Technologies provides multiple types of DNA templates for in vitro transcription, including the linearized DNA plasmids, PCR products, synthetic oligos, and cDNA.
Expert Synthesis with Comprehensive Quality Control
* Please see a denaturing agarose gel example of ssRNA (1010 nt).
For more QC options, please contact us at firstname.lastname@example.org.
RNA (1010nt) on denaturing agarose gel. The RNA was synthesized by IVT and purified via LiCl purification method.