Another strategy of RNA synthesis is by in vitro transcription, especially suitable for long RNA synthesis.
In vitro transcription takes linear DNA sequences as a template, using T7, T3, or SP6 RNA polymerase to synthesize RNA from the DNA sequence.
Synbio Technologies provides highly efficient and cost-effective in vitro transcription RNA synthesis services. You can provide us with the DNA templates, such as plasmid and PCR product, or we can help conduct the de novo gene synthesis of the target DNA sequences. In our design, we synthesize the T7 promoter before the target sequence, which is used to initiate the transcription reaction during the RNA synthesis process. For mRNA transcription, Synbio Technologies provides optional mRNA modifications, such as using mRNA capping enzymes from the vaccinia virus to add cap structures or using E. coli poly(A) polymerase to add poly(A) tails of the transcript RNA. Moreover, our RNA specialty service also includes sgRNA synthesis, you can use the synthesized sgRNA to transform the cell along with Cas9 proteins or Cas9 mRNA to perform downstream targeting.
The Synbio-Tech Difference
- Abundant RNA Types: We offer transcription for a variety of RNA types, including sgRNA, lncRNA, dsRNA, and mRNA, giving you the flexibility to pursue your specific research goals.
- Sequence Optimization: Our NGCodon optimization technology improves mRNA translation efficiency, making your experiments more reliable and reproducible.
- Customization: Our RNA synthesis services are fully customizable, allowing you to tailor your experiments to your specific needs. Choose from a wide range of options, including 40-9000nt RNA synthesis at microgram-to-gram-scale quantity.
- Stable Modification: Our modifications, including 5′ caps, 3′ polyA tails, and nucleoside modifications, enhance mRNA stability and translation efficiency, while also reducing immunogenicity.
- One-Stop Service: We provide a complete set of services from DNA template synthesis to RNA transcription in vitro, streamlining your research process and saving you time and resources. Trust us to be your one-stop-shop for in vitro transcription.
In Vitro Transcription Process
Templates for In Vitro Transcription
dsDNA templates often increase transcription yield of the RNA. Synbio Technologies provides multiple types of DNA templates for in vitro transcription, including the linearized DNA plasmids, PCR products, synthetic oligos, and cDNA.
Service Specifications
Expert Synthesis with Comprehensive Quality Control
| QC Items* | Method | Specifications | Default QC | QC Price |
|---|---|---|---|---|
| Appearance | Visual inspection | Clear, colorless, free from visible particulates |
√ |
Free |
| A 260/280 Ratio | UV Absorbance | 1.70 ~ 2.30 |
√ |
Free |
| Quantity | UV Absorbance | Quantity is ± 5% |
√ |
Free |
| RNA size & purity* | Denaturing agarose gel electrophoresis | Detect expected size band |
√ |
Free |
| Endotoxin | TAL assay | < 10 EU/mg |
x |
$50 per reaction |
| DNA template residue test | PCR & Gel electrophoresis | PCR product from the RNA products is <5% compared to positive control |
X |
$125 per reaction |
| Bioburden | Streak inoculation | No growth after 48 hours. |
X |
$50 per reaction |
| Mycoplasma | Mycoplasma PCR Detection | No mycoplasma is detected in the RNA products | X | $125 per reaction |
| RNA Sequence confirm | RT-PCR & Sanger sequencing | Sequencing results are consistent with the confirmed RNA sequence |
X |
Starting at $250 |
| Functional test | Transfection & Western Blot | Detect expected size protein |
X |
Contact us |
* Please see a denaturing agarose gel example of ssRNA (1010 nt).
For more QC options, please contact us at quote@synbio-tech.com.
RNA (1010nt) on denaturing agarose gel. The RNA was synthesized by IVT and purified via LiCl purification method.
