Another strategy of RNA synthesis is by in vitro transcription, especially suitable for long RNA synthesis.
In vitro transcription takes linear DNA sequences as a template, using T7, T3, or SP6 RNA polymerase to synthesize RNA from the DNA sequence.
Synbio Technologies provides highly efficient and cost-effective in vitro transcription RNA synthesis services. You can provide us with the DNA templates, such as plasmid and PCR product, or we can help conduct the
de novo gene synthesis of the target DNA sequences. In our design, we synthesize the T7 promoter before the target sequence, which is used to initiate the transcription reaction during the RNA synthesis process. For mRNA transcription, Synbio Technologies provides optional mRNA modifications, such as using mRNA capping enzymes from the vaccinia virus to add cap structures or using E. coli poly(A) polymerase to add poly(A) tails of the transcript RNA. Moreover, our RNA specialty service also includes sgRNA synthesis, you can use the synthesized sgRNA to transform the cell along with Cas9 proteins or Cas9 mRNA to perform downstream targeting.
The Synbio-Tech Difference
- Performance: Our RNA synthesis services are designed to deliver high performance, with a focus on streamlining experimental procedures and providing optimized and tailored solutions. Our professional staff and expertise ensure that we can deliver the best possible results for your research needs, with a commitment to quality and reliability. We understand that every research project is unique, which is why we offer customized solutions that are tailored to your exact specifications, delivering the performance you need to succeed.
- Customization: Our RNA synthesis services are fully customizable to meet your specific research needs, offering a wide range of options from 100-8000nt RNA synthesis at microgram-to-gram-scale quantity. We also provide services for single modification and design-to-detection, ensuring that you have full control over the final product. Our team is committed to delivering customized solutions that meet your exact requirements, with a focus on quality and reliability.
- Quality Control: We maintain a rigorous ISO9001 quality management system to ensure that our RNA synthesis services meet the highest standards of quality and reliability. Additionally, we offer optional custom assay development and validation to ensure that your final product meets your exact specifications. Our commitment to quality control ensures that you can rely on our RNA synthesis services for even the most demanding research applications.
- Turnaround : We understand the importance of fast turnaround time, which is why we offer RNA synthesis services that can be completed in as little as 5 business days, provided that you provide us with DNA templates. Our streamlined process and advanced technology enable us to deliver high-quality RNA synthesis services quickly and efficiently, without compromising on quality or reliability. You can trust us to deliver your final product on time, every time.
In Vitro Transcription Process
Templates for In Vitro Transcription
dsDNA templates often increase transcription yield of the RNA. Synbio Technologies provides multiple types of DNA templates for in vitro transcription, including the linearized DNA plasmids, PCR products, synthetic oligos, and cDNA.
In Vitro Transcription Service Specifications
| Gene Length | Order Size | Price | Turnaround Times (Business Days) |
Deliverables |
|---|---|---|---|---|
| 20-1,500 bp | 10ug-50ug | Starting from $250 | 10-15 |
|
| 1mg-5mg | Starting from $3,500 | |||
| 1g | Inquiry | Inquiry | ||
| 1,501-3,000 bp | 10ug-50ug | Starting from $250 | 15-20 | |
| 1mg-5mg | Starting from $3,500 | |||
| 1g | Inquiry | Inquiry | ||
| 3,001 bp-4,500 | 10ug-50ug | Starting from $250 | 20-25 | |
| 1mg-5mg | Starting from $3,500 | |||
| 1g | Inquiry | Inquiry | ||
| 4,501-27,000 bp | 10ug-1g | Inquiry | Inquiry |
*1 For more information please contact us at quote@synbio-tech.com.
*2 The price only refers to the transcription, gene synthesis price and modification will be charged separately.
*3 The turnaround time contains gene synthesis and IVT; If you provide the DNA template, the IVT can be completed in as fast as 5 business days.
Expert RNA Transcript Synthesis with Comprehensive Quality Control
At Synbio Technologies, we utilize cutting-edge T7 RNA polymerase technology to produce high-quality RNA transcripts. Our RNA synthesis services are complemented by a comprehensive quality control program that includes agarose gel analysis to ensure IVT RNA purity and size. In addition to our standard RNA synthesis services, we offer a range of custom options that can be tailored to meet your specific project requirements. Our team of experts is committed to delivering reliable, high-performance RNA synthesis services for your research or industrial applications.
Quality Control Features
- OD260/280
- Fragment size
- Appearance
- Native agarose gel
- Denature agarose Gel
- Reliable lab report with timely update
- DNA template residue test
- Mycoplasma contamination test
- RNA Sequence confirmation
