Another strategy of RNA synthesis is by in vitro transcription, especially suitable for long RNA synthesis.

In vitro transcription takes linear DNA sequences as a template, using T7, T3, or SP6 RNA polymerase to synthesize RNA from the DNA sequence.

Synbio Technologies provides highly efficient and cost-effective in vitro transcription RNA synthesis services. You can provide us with the DNA templates, such as plasmid and PCR product, or we can help conduct the de novo gene synthesis of the target DNA sequences. In our design, we synthesize the T7 promoter before the target sequence, which is used to initiate the transcription reaction during the RNA synthesis process. For mRNA transcription, Synbio Technologies provides optional mRNA modifications, such as using mRNA capping enzymes from the vaccinia virus to add cap structures or using E. coli poly(A) polymerase to add poly(A) tails of the transcript RNA. Moreover, our RNA specialty service also includes sgRNA synthesis, you can use the synthesized sgRNA to transform the cell along with Cas9 proteins or Cas9 mRNA to perform downstream targeting.

The Synbio-Tech Difference

  • Abundant RNA Types: We offer transcription for a variety of RNA types, including sgRNA, lncRNA, dsRNA, and mRNA, giving you the flexibility to pursue your specific research goals.

  • Sequence Optimization: Our NGCodon optimization technology improves mRNA translation efficiency, making your experiments more reliable and reproducible.

  • Customization: Our RNA synthesis services are fully customizable, allowing you to tailor your experiments to your specific needs. Choose from a wide range of options, including 40-9000nt RNA synthesis at microgram-to-gram-scale quantity.

  • Stable Modification: Our modifications, including 5′ caps, 3′ polyA tails, and nucleoside modifications, enhance mRNA stability and translation efficiency, while also reducing immunogenicity.

  • One-Stop Service: We provide a complete set of services from DNA template synthesis to RNA transcription in vitro, streamlining your research process and saving you time and resources. Trust us to be your one-stop-shop for in vitro transcription.

In Vitro Transcription Process

Templates for In Vitro Transcription

dsDNA templates often increase transcription yield of the RNA. Synbio Technologies provides multiple types of DNA templates for in vitro transcription, including the linearized DNA plasmids, PCR products, synthetic oligos, and cDNA.

Service Specifications

  • 40 – 9000 nt
  • 2 µg – g scale
  • 2 µg start from $200
  • 1 mg start from $1,000

* The price only refers to the transcription, gene synthesis price and modification will be charged separately.

  • IVT: 5~10 BD.
  • Gene Synthesis: 10~15 BD.
  • Liquid RNA
  • COA file
  • Lyophilized plasmid
  • 5-mUTP
  • 5-moUTP
  • ψ-UTP
  • N1-Me-ψ-UTP
  • Cy3, Cy5, AF488
  • Unmodified
  • One stop capping (Clean cap AG, ARCA cap, m7GpppG Cap)
  • Enzymatic capping (Cap 0, 1)
  • No cap
  • Customized tail
  • 100~150A tail (enzymatic method)
  • 120A tail
  • No tail
  • Silica column purification
  • LiCl purification
  • Phenol/chloroform extraction
  • HPLC


Expert Synthesis with Comprehensive Quality Control




QC Items*



Default QC

QC Price


 Visual inspection

Clear, colorless, free from visible particulates


A 260/280 Ratio

UV Absorbance

1.70 ~ 2.30



UV Absorbance

Quantity is ± 5%


RNA size & purity*

Denaturing agarose gel electrophoresis

Detect expected size band



TAL assay

< 10 EU/mg


$50 per reaction

DNA template residue test

PCR & Gel electrophoresis

PCR product from the RNA products is <5% compared to positive control


$125 per reaction


Streak inoculation

No growth after 48 hours.


$50 per reaction


Mycoplasma PCR Detection

No mycoplasma is detected in the RNA products

X $125 per reaction

RNA Sequence confirm

RT-PCR & Sanger sequencing

Sequencing results are consistent with the confirmed RNA sequence


Starting at $250

Functional test

Transfection & Western Blot

Detect expected size protein


Contact us

* Please see a denaturing agarose gel example of ssRNA (1010 nt).
For more QC options, please contact us at

RNA gel

RNA (1010nt) on denaturing agarose gel. The RNA was synthesized by IVT and purified via LiCl purification method.

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