Synthesis and Assembly Services on Gene Cluster and Small Genome

Currently, Gibson assembly is the most popular way to achieve directed cloning and in vitro multiple-segment assembly. However, Gibson assembly is limited to plasmids around 10Kb or less in length, which largely restricts its applications.

A number of researchers found that assembly in yeast is highly potential as one of the convenient approaches to assemble DNA segments. Yeast can connect multiple DNA segments automatically without DNA polymerases and ligases, making it possible to construct a plasmid over 10Kb with fast turnaround. Yeast homologous recombination technology is therefore hugely beneficial for fast and high-efficient assembly of long DNA fragments and genomes.

DNA Assembly Technologies Gibson Assembly Method Yeast Homologous Recombination Technology
DNA polymerases/Taq ligases Required Not required, yeast uses homologous recombination
Multiple fragment assembly Seamless assembly One-step Assembly
Long DNA fragments and genomes Fragments over 20Kb are hard to achieve Able to assemble fragments up to 1.08Mb

By utilizing yeast homologous recombination technology, Synbio Technologies is able to provide one-stop services for long gene synthesis/assembly and gene cluster/small genome synthesis and assembly. These services are accomplished with our unique Syno®2.0 and 3.0 Synthesis Platforms. To ensure overall quality we guarantee 100% accuracy on all our delivered DNA products.

Figure 1. Diagram of the synthesis and assembly platform of gene clusters and small genomes

Competitive Advantages

Powerful Syno®2.0 and Syno®3.0 Gene Synthesis Platforms provide one-stop solutions for yeast assembly platforms such as gene synthesis, assembly and sequencing.

Synthesis and assembly of long DNA fragments with high fidelity and speed. Synbio Technologies can deliver single gene sequences up to 150 Kb, guaranteeing reliable assembly of long DNA fragments.

Service Specifications

Services Details Turnaround Time Delivered Product Price
Synthesis of gene clusters and small genomes Customized service for metabolic pathway synthesis <10Kb, 4-6 weeks; >10Kb, Inquiry Synthetized gene clusters, plasmids with small genomes and glycerol bacteria Inquire