Plasmid DNA preparation is a basic but very important step in scientific research. For years, Synbio Technologies has provided comprehensive plasmid DNA preparation services to our customers from research institutes to both biotechnology and biopharmaceutical industries.
Synbio Technologies conducts comprehensive quality control of our plasmid products to provide you with aseptic plasmids with no RNA pollution or genome pollution. All plasmids we manufacture are free of animal-derived materials and can contain low levels of endotoxin (< 100EU/mg, <30EU/mg, <5EU/mg, on request). According to our customers’ application needs, standard plasmid DNA preparation services are divided into two levels, the research level and the transfection level. The plasmid DNA preparation process is designed to meet diverse downstream applications such as transfection, antibody preparation, vaccine, and gene-therapy research.
Competitive Advantages
- Express and Guaranteed Plasmid Preparation Services: USA based manufacturing allows high quality products and fast delivery.
- Highly Customized: Microgram-to-gram-scale quantity can meet various customers’ needs.
- Low Endotoxin Level: Animal-derived materials free, <100EU/mg, on request.
- Strict Quality Control: ISO9001 & ISO13485 quality management, complete and detailed manufacturing documentation.
- Intellectual Property (IP) Protection: All our customers’ intellectual project related rights are fully respected and protected.
Service Process
Size | TAT | Endotoxin Level | Price | |
Research Grade | 100-200 μg | 5-8 BD | < 0.1EU/μg <0.05EU/μg |
Quote |
200-500 μg | 5-8 BD | |||
500-1000 μg | 5-8 BD | |||
HT Grade | 15 μg | 5-8 BD | ||
GMP-Like Grade | 1-10 mg | 6-9 BD | ||
10-50 mg | 9-13 BD | |||
50 mg-1 g | Quote | |||
GMP Grade | 100 mg-10 g | Quote | < 0.01EU/μg |
Synbio Technologies plasmid preparation service focuses on quality. We have strict quality control which can increase quality stability and reduce batch difference to satisfy customers’ high-quality needs.
QC Items* | Method | Specifications | Default QC Research Grade |
Default QC Transfection Grade |
QC Price |
Appearance | Visual inspection | Clear, colorless, free from visible particulates. | √ | √ | Free |
A 260/280 Ratio | UV Absorbance | 1.80 ~ 2.00. | √ | √ | Free |
Quantity | UV Absorbance | Quantity is ± 5%. | √ | √ | Free |
Residual RNA | Agarose gel electrophoresis | Not visible | √ | √ | Free |
Genomic DNA | Agarose gel electrophoresis | Not visible | √ | √ | Free |
Fragment Size | Restriction Digestion | The size of plasmids fragment is right and free of any contaminated bands. | √ | √ | Free |
Endotoxin control | TAL assay | < 100EU/mg | x | √ | Free |
Supercoil content | Agarose gel electrophoresis | Supercoil content >90% | x | √ | Free |
Bioburden | Streak inoculation | No growth after 48 hours. | x | √ | Free |
Mycoplasma | Streak inoculation | No mycoplasma is detected in the plasmid products. | x | x | Contact us. |
Sequencing | Whole plasmids sequencing | Sequencing results are consistent with the confirmed plasmid sequence. | x | x | Contact us. |
Contact us for more QC items.