The primary goal of modern drug research and development is to screen, determine, and prepare effective drug targets. CRISPR-Cas9 can efficiently and precisely edit genes in multiple types of cells and tissues. According to the most recent literature, gene editing technology based on the CRISPR-Cas9 presents great potential and prospect for the application in a series of drug targets screening.

By constructing a sgRNA library, Synbio Technologies can use lentivirus-transfected cells to screen the large-scale protein and its domain, all of which can play an important role in the process of disease occurrence and maintaining the cancer cell growth, survival, and development. Moreover, we can then combine this with NGS and data analysis, which is especially important for finding the right drug targets and developing new drugs, especially cancer treatments.

Research Design

Design of screening library

  • Gene retrieval & sgRNA design of the genome or scope panel

Construction of screening library

  • Syno® 2.0 gene synthesis and vector construction
  • sgRNA library construction
  • Preparation of Lentivirus library preparation

High-throughput High-content screening

  • Detection of cell viability, proliferation and apoptosis, etc.
  • Cells screening through immunoassay and Fluorescence Activated Cell Sorting (FACS), etc.

Target screening and confirmation

  • Analyzing and obtaining the genomic information before / after screening by NGS and bioinformatics
  • Obtaining the candidate genes corresponding to sgRNA
  • Construction of subsequent cell line or animal model

Advantages of Screening Services for Research & Development of New Drugs

  • One-stop Service
    Synbio Technologies provides an integrated solution for sgRNA design, sgRNA library construction, lentivirus packaging, target screening and subsequent verification.
  • Patented Next Generation Gene Synthesis Platform
    High-throughput and low-cost sgRNA library construction based on the chip- synthesis technology, the coverage of constructed library is more than 95%.
  • High Throughput Screening Platform
    Synbio Technologies can offer a whole genome library screening and parallel analysis of gene functions of a large number of cells, including cell viability, proliferation, apoptosis and other phenotype detection. It can also identify relevant candidate genes.
  • Biological Information Analysis
    Experienced bioinformatics analysis team, robust technical support, and in-depth analysis of NGS data to precisely select target candidate genes.

[1] Parnas, O., et al., A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks. Cell, 2015. 162(3): p. 675-86.
[2] Sanjana, N.E., Genome-scale CRISPR pooled screens. Anal Biochem, 2016.
[3] Varshney, G.K., et al., A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish. Nat Protoc,2016. 11(12): p. 2357-2375.