It is helpful to boost the efficiency of crop oriented genetic programming by using gene editing techniques to delete unfavorable genes and improve the target characters accurately. As a benefit from its convenient, accurate, and multi-targets gene editing function, CRISPR-Cas9 has successfully been applied in plant genes to improve the phenotypic characteristics against insect pests and drought. It is expected to be widely used in precise genetic improvement of crops. Precise plant gene editing has also been listed as one of the ten major scientific and technological breakthroughs in 2016. The targeted gene engineering based on CRISPR-Cas9 has gradually replaced traditional plant breeding and genetically modified organism (GMO) methods to improve crop characteristics to ensure the sustainable production of crops.