It is helpful to boost the efficiency of crop oriented genetic programming by using gene editing techniques to delete unfavorable genes and improve the target characters accurately. As a benefit from its convenient, accurate, and multi-targets gene editing function, CRISPR-Cas9 has successfully been applied in plant genes to improve the phenotypic characteristics against insect pests and drought. It is expected to be widely used in precise genetic improvement of crops. Precise plant gene editing has also been listed as one of the ten major scientific and technological breakthroughs in 2016. The targeted gene engineering based on CRISPR-Cas9 has gradually replaced traditional plant breeding and genetically modified organism (GMO) methods to improve crop characteristics to ensure the sustainable production of crops.

Application of CRISPR-Cas9 in Plant Genome Editing

Research Strategy

sgRNA and Cas9 design

  • Gene transfer and sgRNA design within genome or panel
  • Codon optimization for Cas9 to enhance translation in plant cells

sgRNA and Cas9 construction

  • Syno® 2.0 gene synthesis and vectors construction platform
  • Syno® 3.0 sgRNA library construction platform
  • Vector design for multi-mode species


Verification and analysis of
functions in vitro

  • SSA activity Detection
  • Cleavage in vitro Activity detection
  • Endogenous active cleavage


  • One-stop solution
    Synbio Technologies provides complete solutions from sgRNA design, Cas9 codon optimization, sgRNA and Cas9 synthesis and constructionto activity detection.
  • Patented technology platform
    Synbio Technologies’ patented sgRNA design software provides efficient and accurate designs for multiple species.
  • Patented NG® Codon optimization
    Optimize Cas9 protein to ensure its efficient translation in plant cells.