Using CRISPR/Cas9 for genome editing, allowing to precisely edit the genome of cells by inducing double-stranded DNA (dsDNA) breaks at specific loci, is both an efficient and cost-effective technological tool. sgRNA serves as a guide to recognize and combine target with DNAs, allowing activated Cas9 nuclease to attach, cleave, and modify the PAM downstream target DNA. CRISPR-based genome editing technology has made it easier and faster than ever to alter specific DNA sequence in the genome or to perform genome-wide functional screening tests to identify genes involved in a particular phenotype. CRISPR-Cas9 is considered as the most promising tool in gene modification.
PAM: Protospacer Adjacent Motif
DSB: Double Strand Break
NHEJ: Non-Homologous End Joining
HDR: Homology Directed Repair
GFP: Green Fluorescent Protein
The sgRNA design center of Synbio Technologies provides services such as single sequence sgRNA design or whole-genome sgRNA library design, downstream verification, and stable cell line construction. We offer a one-stop solution for CRISPR-Cas9 projects to achieve high genome editing efficiency.
CRISPR-Cas9 sgRNA Design Services