CRISPR technology has become one of the most effective method for gene/genome editing. CRISPR library/sgRNA library can simultaneously edit multiple genes in a signal pathway or an entire genome, which is a powerful research tool in CRISPR screening. Through functional screening and enrichment, PCR amplification, and deep sequencing analysis, the functional gene can be identified and the related signal pathway and molecular mechanism can be recognized. This technology has been widely used in cancer diagnosis, drug discovery, etc.
Synbio Technologies has accumulated more than ten years of CRISPR library experience. We can provide global customers with one-stop, precise, efficient, and customized solutions, including sgRNA design, oligo pool synthesis, CRISPR library/sgRNA library construction, NGS validation, high-content screening, etc.
CRISPR Library/sgRNA Library Process
CRISPR Library/sgRNA Library Construction and Screening Workflow
CRISPR Library/sgRNA Library Screening Case Study
Screening resistance targets for a drug with identified function