Oligonucleotide synthesis is the chemical synthesis of short nucleic acid fragments with defined sequence. In this process, 2 ‘- deoxynucleosides (dA, dC, dG and dT), ribonucleosides (A, C, G and U) or chemically modified nucleosides (such as LNA) are synthesized by solid-phase phosphoramide method to obtain sequences with specific chemical structure and function. Oligonucleotide synthesis has the characteristics of stable, efficient and rapid synthesis, usually in the form of primers or probes, widely used in PCR amplification, DNA sequencing, molecular diagnosis and many other fields.

Synbio Technologies’ oligonucleotide synthesis platform equips state-of-the-art synthesizers and leading professional teams experienced in the oligo synthesis as well as modification technologies. This allows us to provide high-quality oligo products to scientific researchers and industrial customers around the world. By leveraging our advanced and innovative manufacturing process, Synbio Technologies offers a wide range of oligonucleotide synthesis options, including custom DNA oligos, diagnostic oligos & probes, NGS oligos, modified oligos, antisense oligos, CpG ODNs, etc. In addition, we also provide high-throughput oligo library and oligo pool synthesis to customers with large-scale oligo projects. Synbio Technologies is always competent to offer tailor-made approached to accomplish every request to oligo synthesis.

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Why us?

Certified with the highest manufacturing standards (ISO9001 & ISO13485), stringent QC & QA standards, large-scale sequencing verifying the average mutation rate to be lower than 1/1000.

Various highly customizable options of purification, specifications and delivery forms, and flexible synthesis scale is available upon request all well.

Qualified and available for multiple applications such as molecular diagnosis, chip sequencing, genome engineering, tumor research, forensic identification, precision medicine, IVD, digital information storage, etc.

Standard Oligo Synthesis Process

Based on customers’ various requests, Synbio Technologies’ scientific teams will performance proprietary DNA sequence analysis and design. CpG-based chemical oligo synthesis will be finished by utilizing industry leading manufacture process. For final sequence verification, MS analysis and other stringent quality control method will be conducted step by step.

Oligo Synthesis Services

Service Options Specifications Deliverables
Custom DNA Oligos Providing standard unmodified DNA oligo synthesis. It includes standard oligos, micro-scale oligos, large-scale oligos, degenerate primers, as well as long oligos.

Standard package:

  1. Lyophilized Dry powder: 2 OD oligo, 1 tube (transparent or dark)
  2. Certificate of Analysis (COA): includes sequence information, OD, Tm, etc.
Diagnostic Oligos & Probes Providing the services of FISH probes, qPCR probes, SNP probes, and other molecular diagnostic materials.
NGS Oligos High quality oligos with enhanced purity and accuracy for Next Generation Sequencing (NGS).
Modified Oligos Comprehensive oligo modification and labeling services, such as special bases, dU/dI, phosphorothioate, phosphorylation, spacers, amino, biotin, FAM, HEX, ROX, BHQ, etc.
Antisense Oligos Strictly comply with QC testing standards and HPLC purity detection is used to ensure the high quality output of all our ASO products.
CpG ODNs Providing three types of CpG ODNs (Class A, Class B, and Class C) of various species.
Oligo Libraries Using high-throughput semiconductor chip synthesis technology to provide customers with the design and synthesis of various library oligos, including oligo pools, trimer oligos, and sgRNA library oligos.
Oligo Pools High throughput oligo synthesis, up to 1 million oligos can be synthesized on a single chip within one synthetic circle.